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Increased Mitochondrial DNA Copy Number in Occupations Associated with Low-Dose Benzene Exposure.
In: Environmental Health Perspectives, Jg. 120 (2012-02-01), Heft 2, S. 210-215
Online
academicJournal
Zugriff:
BACKGROUND: Benzene is an established leukemogen at high exposure levels. Although low-level benzene exposure is widespread and may induce oxidative damage, no mechanistic biomarkers are available to detect biological dysfunction at low doses. OBJECTIVES: Our goals were to determine in a large multicenter cross-sectional study whether low-level benzene is associated with increased blood mitochondrial DNA copy number (mtDNAcn, a biological oxidative response to mitochondrial DNA damage and dysfunction) and to explore potential links between mtDNAcn and leukemia-related epigenetic markers. METHODS: We measured blood relative mtDNAcn by real-time polymerase chain reaction in 341 individuals selected from various occupational groups with low-level benzene exposures (> 100 times lower than the Occupational Safety and Health Administration/European Union standards) and 178 referents from three Italian cities (Genoa, Milan, Cagliari). RESULTS: In each city, benzene-exposed participants showed higher mtDNAcn than referents: mtDNAcn was 0.90 relative units in Genoa bus drivers and 0.75 in referents (p = 0.019); 0.90 in Milan gas station attendants, 1.10 in police officers, and 0.75 in referents (p-trend = 0.008); 1.63 in Cagliari petrochemical plant workers, 1.25 in referents close to the plant, and 0.90 in referents farther from the plant (p-trend = 0.046). Using covariate-adjusted regression models, we estimated that an interquartile range increase in personal airborne benzene was associated with percent increases in mtDNAcn equal to 10.5% in Genoa (p = 0.014), 8.2% (p = 0.008) in Milan, 7.5% in Cagliari (p = 0.22), and 10.3% in all cities combined (p < 0.001). Using methylation data available for the Milan participants, we found that mtDNAcn was associated with LINE-1 hypomethylation (-2.41%; p = 0.007) and p15 hypermethylation (+15.95%, p = 0.008). CONCLUSIONS: Blood MtDNAcn was increased in persons exposed to low benzene levels, potentially reflecting mitochondrial DNA damage and dysfunction. [ABSTRACT FROM AUTHOR]
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Increased Mitochondrial DNA Copy Number in Occupations Associated with Low-Dose Benzene Exposure.
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Autor/in / Beteiligte Person: | Carugno, Michele ; Pesatori, Angela Cecilia ; Dioni, Laura ; Hoxha, Mirjam ; Bollati, Valentina ; Albetti, Benedetta ; Byun, Hyang-Min ; Bonzini, Matteo ; Fustinoni, Silvia ; Cocco, Pierluigi ; Satta, Giannina ; Zucca, Mariagrazia ; Merlo, Domenico Franco ; Cipolla, Massimo ; Bertazzi, Pier Alberto ; Baccarelli3, Andrea |
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Zeitschrift: | Environmental Health Perspectives, Jg. 120 (2012-02-01), Heft 2, S. 210-215 |
Veröffentlichung: | 2012 |
Medientyp: | academicJournal |
ISSN: | 0091-6765 (print) |
DOI: | 10.1289/ehp.1103979 |
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