The extent to which immunity, apoptosis and detoxification gene expression interact with 17 alpha-methyltestosterone.
In: Fish & Shellfish Immunology, Jg. 60 (2017), S. 289-298
academicJournal
Zugriff:
Innate immunity is the first line of defence against invasion by foreign pathogens. One widely used synthetic androgen for the production of all-male fish, particularly commercially valuable Nile tilapia, Oreochromis niloticus , is 17 alpha-methyltestosterone (MT). The present study investigates the effect of MT on innate immunity, cellular apoptosis and detoxification and the mortality rate, during and after the feeding of fry with 0-, 40-and 60-mg MT/kg. Expression analysis was completed on interleukin 1 beta ( il1β ), interleukin 8 ( il8 ), tumour necrosis factor alpha ( tnfα ), CXC2 - and CC -chemokines, interferon ( ifn ), myxovirus resistance ( mx ), toll-like receptor 7 ( tlr7 ), immunoglobulin M heavy chain ( IgM heavy chain), vitellogenin ( vtg ), cellular apoptosis susceptibility ( cas ) and glutathione S -transferase α1 ( gstα1 ). Expression analysis revealed that MT had a significant impact on these genes, and this impact varied from induction to repression during and after the treatment. Linear regression analysis showed a significant association between the majority of the tested gene transcript levels and mortality rates on the 7 th and 21 st days of hormonal treatment and 2 weeks following hormonal cessation. The results are thoroughly discussed in this article. This is the first report concerning the hazardous effect of MT on a series of genes involved in immunity, apoptosis and detoxification in the Nile tilapia fry. [ABSTRACT FROM AUTHOR]
Titel: |
The extent to which immunity, apoptosis and detoxification gene expression interact with 17 alpha-methyltestosterone.
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Autor/in / Beteiligte Person: | Abo-Al-Ela, Haitham G. ; El-Nahas, Abeer F. ; Mahmoud, Shawky ; Ibrahim, Essam M. |
Zeitschrift: | Fish & Shellfish Immunology, Jg. 60 (2017), S. 289-298 |
Veröffentlichung: | 2017 |
Medientyp: | academicJournal |
ISSN: | 1050-4648 (print) |
DOI: | 10.1016/j.fsi.2016.11.057 |
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