Combination of t(4;14), del(17p13), del(1p32) and 1q21 gain FISH probes identifies clonal heterogeneity and enhances the detection of adverse cytogenetic profiles in 233 newly diagnosed multiple myeloma.
In: Molecular Cytogenetics (17558166), Jg. 10 (2017-07-01), S. 1-6
Online
academicJournal
Zugriff:
Background: Our aim was to set the FISH combination of del(17p13), t(4;14), 1q21 gain and del(1p32), four adverse cytogenetic factors rarely evaluated together, and compare our technical thresholds with those defined in the literature. Methods: Two hundred thirty-three patients with MM at diagnosis were studied using FISH to target 4 unfavorable cytogenetic abnormalities: 17p13 deletion, t(4;14) translocation, 1p32 deletion and 1q21 gain. Technical thresholds were determined for each probe using isolated CD138-expressing PC from patients without MM. Results: The FISH analysis identified abnormalities in 79.0% of patients. Del(17p13) was detected in 15.0% of cases, t(4;14) in 11.5%, 1q21 gain in 37.8% and del(1p32) in 8.7%. Adding 1p32/1q21 FISH probes has enabled us to identify adverse cytogenetic profiles in 39.0% of patients without del(17p13) or t(4;14). Clonal heterogeneity was observed in 51.1% of patients as well as an increase in the number of adverse abnormalities when related clones were greater than or equal to 2 (85.1% against 45.6%). Conclusion: FISH allowed detecting accumulation of adverse abnormalities and clonal heterogeneity in MM with a combination of 4 probes. The impacts of these two parameters need to be evaluated, and could be included in future cytogenetic classifications. [ABSTRACT FROM AUTHOR]
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Combination of t(4;14), del(17p13), del(1p32) and 1q21 gain FISH probes identifies clonal heterogeneity and enhances the detection of adverse cytogenetic profiles in 233 newly diagnosed multiple myeloma.
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Autor/in / Beteiligte Person: | Smol, Thomas ; Dufour, Annika ; Tricot, Sabine ; Wemeau, Mathieu ; Stalnikiewicz, Laure ; Bernardi, Franck ; Terré, Christine ; Ducourneau, Benoît ; Bisiau, Hervé ; Daudignon, Agnès |
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Zeitschrift: | Molecular Cytogenetics (17558166), Jg. 10 (2017-07-01), S. 1-6 |
Veröffentlichung: | 2017 |
Medientyp: | academicJournal |
ISSN: | 1755-8166 (print) |
DOI: | 10.1186/s13039-017-0327-3 |
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