EFFECTS OF TRANSPORT AND HOLDING STRESS ON PRUSSIAN CARP (Carassius gibelio, Bloch, 1782.) LEUKOGRAM PATTERN.

Stress inevitably occurs during any fish handling and manipulation in culturing, research, or clinical examination situations that require capture and removal of fish from water. Different stress factors can affect the changes in the relative numbers and function of cells of the fish immune system. Catching, transportation and over-crowding caused stress-induced changes in the total number of leukocytes and thrombocytes, as well as changes in the leukocyte formula in Prussian carp (Carassius gibelio, Bloch, 1782) presented here. Cytochemical characterization of leukocyte cell types was performed by applying Myeloperoxidase (MPO), Periodic Acid Schiff (PAS) and Sudan Black B (SBB) staining of Prussian carp blood smears. Cytochemical characterization is a rapid and efficient method for white leukocyte differentiation and insight in their functional status. Comparison and analysis in Prussian carp hematological parameters from fish with and without exposure to stressful conditions such as capture, manipulation, transport and holding, revealed significant differences between stressed and non-stressed fish. Significant reduction in the total number of thrombocytes and lymphocytes and the increase in total neutrophil count were observed in stressed animals. However, differences in total leukocyte number and the number of monocytes were not observed. Deviations from the estimated reference intervals for Prussian carp hematological parameters clearly indicated the presence/absence of a stress reaction and to some extent its intensity. Estimated reference intervals and characterization of morphological and cytochemical appearance of blood cells form a solid basis for further research of the cellular immune function and hematology changes in Prussian carp. [ABSTRACT FROM AUTHOR]

Stres je nerazdvojni deo akvakulture, istraživanja, kliničkog pregleda, kao i svih ostalih manipulacija ribom, pošto svi postupci zahtevaju vađenje ribe iz vode. Različiti faktori stresa imaju uticaj na zastupljenost i funkcije ćelija imunskog sistema. Našim istraživanjima bile su obuhvaćene promene ukupnog broja leukocita i trombocita, kao i promene u leukocitarnoj formuli kod babuški (Carassius gibelio, Bloch, 1782.) koje su izložene uticaju stresa usled izlovljavanja, manipulacije, transporta i prenaseljenosti. Posebna pažnja posvećena je citohemijskoj karakterizaciji ovih tipova ćelija kod babuški primenom mijeloperoksidaza (MPO), Periodic acid Schiff (PAS) i Sudan Black B (SBB) bojenja. Poređenje i analiza ispitivanih parametara kod riba koje su bile izložene faktorima stresa sa onima koje nisu, ukazuje na postojanje značajnih razlika pojedinih parametara. Posle izlaganja stresu došlo je do značajnog smanjenja ukupnog broja trombocita i limfocita a povećanja kod broja neutrofila. Nisu primećene značajne razlike u ukupnom broju leukocita kao ni u broju monocita. Dobijeni referentni intervali i morfološki izgled obezbeđuju osnovu za dalja istraživanja funkcije i promene krvnih ćelija babuški dok brza citohemijska bojenja predstavljaju efikasne metode za diferencijaciju različitih tipova leukocita kod riba. Odstupanje od utvrđenih vrednosti za pomenute hematološke parametre omogućilo je da se utvrdi prisustvo stresne reakcije i delimično njen intenzitet. [ABSTRACT FROM AUTHOR]