Advances in Polymerase Chain Reaction on Microfluidic Chips.
In: Analytical Chemistry, Jg. 77 (2005-06-15), Heft 12, S. 3887-3894
academicJournal
Zugriff:
The article discusses advances in polymerase chain reation on microfluidic chips. The mapping and sequencing of the human genome represents a monumental accomplishment that will impact medicine and biology in ways that people are only beginning to comprehend. The successful sequencing reported in 2001 ushered in what has been dubbed the "postgenome era". While this implies that the genome era is over, it is, in fact, just getting underway in so many ways. A map of the more than 3 billion bases and their corresponding 30000+ genes will not only be critical in fleshing out the structure and function of these genes but will leverage the discovery of new genes and a newfound understanding of the potential roles the gene products play in health and disease. Genetic analytical methods have been, and will continue to be, critical to such discovery, providing the means to dissect gene structure and gene function. These methods have almost invariably involved two general processes: sample preparation and separation/detection. Capillary electrophoresis (CE), a means for separating DNA fragments in a manner that allows for decoding DNA sequence, has supplanted the tedious, time-consuming methods associated with sequencing slab gels. As a consequence of this, the role of CE in accelerating the sequencing of the genome is well- recognized.
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Advances in Polymerase Chain Reaction on Microfluidic Chips.
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Autor/in / Beteiligte Person: | Roper, Michael G. ; Easley, Christopher J. ; Landers, James P. |
Zeitschrift: | Analytical Chemistry, Jg. 77 (2005-06-15), Heft 12, S. 3887-3894 |
Veröffentlichung: | 2005 |
Medientyp: | academicJournal |
ISSN: | 0003-2700 (print) |
DOI: | 10.1021/ac050756m |
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