茶摄入量与结直肠癌发病因果关系的 孟德尔随机化研究.

Objective To study the(*ausal ndationship between ten intakes and the incidence of colorectal cane(江 using two 一 sampler Mcndclian i\.mclonnzation. Methods Using the colomctal cdncex genome - wide association analysis data (GW AS ID： icu - b - 4965) and ten intake genome -賀 ide association analysis data (GW AS ID: ukl)一 h -6066) in the IEU OPEN GW AS public databases, we summarizcxl and scmcncd the two data sets for single nucleotide polymorphisms (SNPs) which 胃cm closely r(dat(xl to and independent of ten intakes as instrnm(niti.d variables (IVs), By using MR 一 Egg(江 and irrverse varian(*e weighting in two - sample M(nid(dian randomization, Fiw: methods (IVW), weighted m(xlian method (Weighted Median), simple mod(d (Simple Mode) and w(dglit(xl model (Weighted Mode) w(te used to (lctcnuinc the(*ansal analysis between ten intakes and the incidence of colorectal ennee匚 OR and 95% CI were, iiscxl to (examine the (*ansal relationship between ten intakes and the incidence of colorectal cimcc匚 MR 一 PRESSO was us(xl for outlicr testing. IVW (Co(*hran Q test) and MR 一 Egget (Riick(江 Q test) wcm iiscxl for heterogeneity analysis. Egget -- intercept was ns(xl for pkdotropy test, sensitivity analysis was p(Tfbrm(xl by -- one -- out method, and funned plot was used to analyze the presence of potential bias. Results 39 SNPs were finally in(dnd(xl, and MR 一 PRESSO foiind no outlier SNPs = P = 0. 237 > 0. 05; in terms of (causality determination: IVW (OR = 0. 996, 95% C/：0. 990-1.002, P= 0. 293), MR - E 弊江(OR =0, 992, 95% CI ：0.977 - 1.007, P= 0. 293), Weighted Median (OR =0, 997, 95% CI： 0. 987 - 1,007, P = 0.479), Simple Mode (OR =0, 996, 95% CZ： 0. 976 - 1.017, P = 0. 724), Weighted Mode (OR =0, 996, 95% C/：0. 985 - 1,007, P =0. 473). The nhovc nidts showcxl that there was no (*ausal rdationship het科ccn ten intakes and the incidence of colorectal eaneex. In the heterogeneity test, P = 0. 181 > 0. 05 in Co(*hran Q test and P = 0. 167 > 0. 05 in Ruck(江 Q test. Them was no signili(*ant heterogeneity. The Egget -- intercept test show(xl that P =0, 489 > 0. 05 and was not plciottopic. The leave -- one -- out method was r(mov(xl one by one, and no SNPs significantly affecting the nsidts were fbnncL The funned plot was hnsically symmetrical, indi(*ating that the main nsidts were ndiablct Conclusion There is no cnusal ndationship between t(血 intakes and the incidence of colorectal cancer. [ABSTRACT FROM AUTHOR]

目的 利用两样本孟德尔随机化研究茶摄人量与结宜肠癌发病的因果关系,, 方法 利用IEU OPEN GWAS公共 数据库中的结直肠癌全基因组关联分析数据(GWAS ID：icu-b-496 5)和茶摄入量全基因组关联分析数据(GWAS ID:nkb-b-606 6),汇总两个数据集, 筛选与茶摄入量密切相关且独立的单核昔酸多态性(SNPs)作为工具变量 (IVs), 通过采用两样本孟德尔随机化中的MR - Egger、逆方差加权(LVW)、加权中位数法(Weighted Median)、简单模 型(Simple Mode)和加权模型(Weighted Mode)五种方法进行茶摄入量与结直肠癌发病的因果分析的判断, 采用OR以 及95% CI判断茶摄入量与结宜肠癌发病是否存在因果关系, 利用MR - PRESSO进行离群值检验, 采用IVW(Cochran Q检验)以及MR - Egger(Rik'ker Q检验)进行异质性分析, 采用Egger - intercept进行多效性检验,通过留一法进行敏 感性分析, 采用漏斗图分析是否存在潜在偏倚"结果 最终纳入39个SNPs, MR - PRESS0未发现离群SNPs,P = 0. 237 >0.05;在因果关系的判定上:IVW(OR = 0. 996,95% CZ：O. 990 ~ 1.002, P = 0. 293), MR - Egger (OR =0. 992, 95%Cl：0. 977 -1.007,P = 0. 293) ,Weighted Median(ORO. 997,95%CZ：O. 987 ~ 1.007,P = 0.479) ,Simple Mode(OR = 0. 996,95%CZ：O. 976 ~ 1.017=0. 724), Weighted Mode(07? = 0. 996,95% Q：0. 985 〜！. 007,P = 0. 473),上述结果显 示:茶摄入量与结直肠癌发病无因果关系, 在异质性检测中Cochran Q检验中P=0. 181 > 0. 05, R iickcr Q检验中P = 0. 167 >0. 05,无显著异质性,Egger - intercept进行多效性检验P =0.489 > 0. 05未见多效性, 留一法逐个剔除,未见明 显影响结果的SNPs,漏斗图基本对称, 显示主结果可靠"结论 茶摄入量与结直肠癌发病无因果关系,. [ABSTRACT FROM AUTHOR]