بـررسی ارتباط بین پـلیمورفیسمهای 6499640rs و 17817449rsدر ژن FTO با بیماری دیابت نوع 2.
In: Jundishapur Scientific Medical Journal, Jg. 22 (2023-11-01), Heft 5, S. 594-606
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Zugriff:
Background and Objectives Diabetes is a growing global health problem caused by the interaction of genetic and environmental factors. One of the most important genes related to type 2 diabetes (T2D) is the FTO gene. The present study aimed to assess the relationship of rs6499640 and rs17817449 polymorphisms in the FTO gene with T2D in Dezful, Khzestan, Iran. Subjects and Methods This research was conducted on 100 healthy people and 100 cases with T2D. DNA extraction was performed after blood sampling. The quantity and quality of extracted DNA were assessed using nanodrop and electrophoresis methods, respectively. Amplification-refractory mutation system and tetra-primer amplification refractory mutation system-polymerase chain reaction (PCR) techniques were used to determine the genotype of samples. Thereafter, we used sequencing to confirm the genotyping results by PCR, and data were analyzed statistically. Results There was no significant relationship in the distribution of genotypes between rs6499640 and rs17817449 polymorphisms of the FTO gene and the increased risk of diabetes in Dezful (P=0.728 and P=0.085, respectively). Nonetheless, in the case of rs17817449 polymorphism, a significant relationship was found between the frequency of GG genotype and the risk of T2D. Conclusion As evidenced by the obtained results, the studied polymorphisms were not correlated with the occurrence of T2D in Dezful. It is recommended that large-scale studies be conducted to confirm these results. [ABSTRACT FROM AUTHOR]
Titel: |
بـررسی ارتباط بین پـلیمورفیسمهای 6499640rs و 17817449rsدر ژن FTO با بیماری دیابت نوع 2.
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Autor/in / Beteiligte Person: | وحیدنیا, شکه ; سیاسی, الهام ; مشایخی, امیر |
Zeitschrift: | Jundishapur Scientific Medical Journal, Jg. 22 (2023-11-01), Heft 5, S. 594-606 |
Veröffentlichung: | 2023 |
Medientyp: | academicJournal |
ISSN: | 2252-052X (print) |
DOI: | 10.22118/jsmj.2023.408821.3190 |
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