AMPK and PGC- α following maximal and supramaximal exercise in men and women: a randomized cross-over study.
In: Applied Physiology, Nutrition & Metabolism, Jg. 49 (2024-04-01), Heft 4, S. 526-538
academicJournal
Zugriff:
We tested the hypothesis that AMPK activation and peroxisome proliferator gamma coactivator 1 alpha (PGC-1α) expression are not augmented as exercise intensity (power output) increases from maximal to supramaximal intensities and conducted an exploratory analysis comparing AMPK activation and PGC-1α expression in males and females. Seventeen (n = 9 males; n = 8 females) recreationally active, healthy, young individuals volunteered to participate in the current study. Participants completed work matched interval exercise at 100% (Max) and 133% (Supra) of peak work rate (WRpeak). Intervals were 1 min in duration and participants were prescribed 6 and 8 intervals of Max and Supra, respectively, to equate external work across protocols. PGC-1α mRNA expression and activation of AMPK (p-ACC) were examined in muscle biopsy samples. Interval WR (watts; W), intensity (%WRpeak) and average HR (bpm), blood lactate (mmol/L) and rating of perceived exertion were all higher (all p < 0.05) in Supra. Fatigue was greater (p < 0.05) in Supra. PGC-1α mRNA expression significantly increased after exercise in Max (p < 0.01) and Supra (p < 0.01), but was not significantly different (p = 0.71) between intensities. A main effect of time (Pre – 0 h) (p < 0.01) was observed for p-ACC; however, no effect of intensity (p = 0.08) or interaction (p = 0.97) was observed. No significant effects of time (p = 0.05) intensity (p = 0.42), or interaction (p = 0.97) were observed for p-AMPK (Thr172). Exploratory sex analysis demonstrated a main effect of sex for p-ACC (greater p-ACC in males; p < 0.05) but not for p-AMPK or PGC-1α expression. Our results confirm that AMPK-PGC-1α signalling is not augmented following supramaximal exercise and provide novel data demonstrating a decrease in AMPK activation (p-ACC) in females compared to men. Trial registration: . [ABSTRACT FROM AUTHOR]
Titel: |
AMPK and PGC- α following maximal and supramaximal exercise in men and women: a randomized cross-over study.
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Autor/in / Beteiligte Person: | Arhen, Benjamin B. ; Renwick, J.R.M. ; Zedic, A.K. ; Menezes, E.S. ; Preobrazenski, N. ; Simpson, C.A. ; Stokes, T. ; McGlory, C. ; Gurd, Brendon J. |
Zeitschrift: | Applied Physiology, Nutrition & Metabolism, Jg. 49 (2024-04-01), Heft 4, S. 526-538 |
Veröffentlichung: | 2024 |
Medientyp: | academicJournal |
ISSN: | 1715-5312 (print) |
DOI: | 10.1139/apnm-2023-0256 |
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