Molecular and Physiological Effects of 17α-methyltestosterone on Sex Differentiation of Black Rockfish, Sebastes schlegelii.
In: Genes, Jg. 15 (2024-05-01), Heft 5, S. 605-619
Online
academicJournal
Zugriff:
It is widely known that all-female fish production holds economic value for aquaculture. Sebastes schlegelii, a preeminent economic species, exhibits a sex dimorphism, with females surpassing males in growth. In this regard, achieving all-female black rockfish production could significantly enhance breeding profitability. In this study, we utilized the widely used male sex-regulating hormone, 17α-methyltestosterone (MT) at three different concentrations (20, 40, and 60 ppm), to produce pseudomales of S. schlegelii for subsequent all-female offspring breeding. Long-term MT administration severely inhibits the growth of S. schlegelii, while short term had no significant impact. Histological analysis confirmed sex reversal at all MT concentrations; however, both medium and higher MT concentrations impaired testis development. MT also influenced sex steroid hormone levels in pseudomales, suppressing E2 while increasing T and 11-KT levels. In addition, a transcriptome analysis revealed that MT down-regulated ovarian-related genes (cyp19a1a and foxl2) while up-regulating male-related genes (amh) in pseudomales. Furthermore, MT modulated the TGF-β signaling and steroid hormone biosynthesis pathways, indicating its crucial role in S. schlegelii sex differentiation. Therefore, the current study provides a method for achieving sexual reversal using MT in S. schlegelii and offers an initial insight into the underlying mechanism of sexual reversal in this species. [ABSTRACT FROM AUTHOR]
Titel: |
Molecular and Physiological Effects of 17α-methyltestosterone on Sex Differentiation of Black Rockfish, Sebastes schlegelii.
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Autor/in / Beteiligte Person: | Huang, Haijun ; Liu, Yuyan ; Wang, Qian ; Dong, Caichao ; Dong, Le ; Zhang, Jingjing ; Yang, Yu ; Hao, Xiancai ; Li, Weijing ; Rosa, Ivana F. ; Doretto, Lucas B. ; Cao, Xuebin ; Shao, Changwei |
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Zeitschrift: | Genes, Jg. 15 (2024-05-01), Heft 5, S. 605-619 |
Veröffentlichung: | 2024 |
Medientyp: | academicJournal |
ISSN: | 2073-4425 (print) |
DOI: | 10.3390/genes15050605 |
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