线粒体 DNA 含量在结直肠癌中的预后价值.

Objective: To investigate the prognostic correlation between mitochondrial DNA (mtDNA) content and colorectal cancer. Methods: Clinical and pathological data were collected from 117 patients with colorectal cancer. RT⁃qPCR was used to determine the mtDNA content of cancer tissues and paracancerous tissues of patients, and the correlation between mtDNA content and various prognostic indicators was investigated. Receiver operating characteristic (ROC) curves were plotted, and the cut-off value was determined to distinguish patients, and disease free survival (DFS) curves were plotted accordingly. Univariate and multivariate Cox regression analyses were conducted to explore the risk factors related to postoperative DFS. Results: There was no significant difference in mtDNA content between cancer tissues and paracancerous tissues (P=0.432); low mtDNA content was associated with tumor location in the colon, poor differentiation, advanced TNM stage, and lymph node metastasis (P < 0.05). The ROC curve indicated a cut⁃off value of 500.699 for mtDNA content. Univariate and multivariate Cox analyses showed that mtDNA content lower than 500.699 (HR=4.285, 95%CI: 1.938-9.475) and poorly differentiated tumors (HR=2.886, 95%CI: 1.428-5.835) were independent risk factors associated with DFS. Conclusion: In patients with colorectal cancer, tissue mtDNA content is associated with clinical and pathological characteristics, and low mtDNA content is an independent risk factor for patient prognosis. [ABSTRACT FROM AUTHOR]

目的: 探究组织线粒体 DNA (mitochondrial DNA, mtDNA) 含量和结直肠癌预后相关性。方法: 选取 117 例结直肠癌 患者并收集临床病理资料。运用 RT⁃qPCR 检测患者癌组织与癌旁组织 mtDNA 含量, 探究 mtDNA 含量与各项预后指标的相关 性。绘制受试者工作特征 (receiver operating characteristic, ROC) 曲线, 根据截断值区分患者并绘制无病生存期 (disease free survival, DFS) 曲线。单因素和多因素 Cox 回归分析探究术后 DFS 相关的危险因素。结果: 与癌旁组织相比, 癌组织 mtDNA 含量 差异无统计学意义 (P=0.432); 低 mtDNA 含量与肿瘤位于结肠、低分化、TNM 分期差、淋巴结转移相关 (P < 0.05)。ROC 曲线提 示 mtDNA 含量为 500.699 可作为截断值。单因素和多因素分析显示, mtDNA 含量低于 500.699 (HR=4.285, 95%CI: 1.938~ 9.475)、肿瘤低分化 (HR=2.886, 95%CI: 1.428~5.835) 是与 DFS 相关的独立危险因素。结论: 结直肠癌患者中, 组织 mtDNA 含量 与临床病理特征有关, 低 mtDNA 含量是患者预后相关的独立危险因素。 [ABSTRACT FROM AUTHOR]