Production, optimization and characterization of partially purified anti-mycotic compound from marine soil derived streptomycetes originating at unexplored region of Bay of Bengal, India.
In: Environmental Research, Jg. 251 (2024-06-20), S. N.PAG
academicJournal
Zugriff:
Sixty-eight morphologically distinct isolates of marine actinomycetes were derived from seashore, mangrove, and saltpan ecosystems located between the Palk Strait and Gulf of Mannar region, Bay of Bengal, Tamilnadu. Twenty-five (36.8%) isolates exhibited anti-mycotic activity against Candida albicans and Cryptococcus neoformans in preliminary screening , and 4 isolates with prominent activity were identified and designated at the genus level as Streptomyces sp. VPTS3-I, Streptomyces sp. VPTS3-2, Streptomyces sp. VPTSA1-4 and Streptomyces sp. VPTSA1-8. All the potential antagonistic isolates were further characterized with phenotypic and genotypic properties including 16S rRNA gene sequencing and identified species level as Streptomyces afghaniensis VPTS3-1, S. matensis VPTS3-2, S. tuirus VPTSA1-4 and S. griseus VPTSA1-8. In addition, the active fractions from the potential antagonistic streptomycetes were extracted with organic solvents by shake flask culture method and the anti-mycotic efficacies were evaluated. The optimization parameters for the production of the anti-mycotic compound were found to be pH between 7 and 8, the temperature at 30ᵒC, the salinity of 2%, incubation of 9 days, and starch and KNO 3 as the suitable carbon and nitrogen sources respectively in starch casein medium. • Sixty-eight marine actinomycetes isolates with different morphologies were obtained. • This study indicated, that with sufficient screening and investigation, marine actinomycetes. • The phenotypic aspects have been discovered to differ in this study. [ABSTRACT FROM AUTHOR]
Titel: |
Production, optimization and characterization of partially purified anti-mycotic compound from marine soil derived streptomycetes originating at unexplored region of Bay of Bengal, India.
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Autor/in / Beteiligte Person: | Vijayakumar, Ramasamy ; Raja, Suresh S.S. ; Muthukumar, Chinnasamy ; Karuppiah, Ponmurugan ; Panneerselvam, Annamalai ; Rajabathar, Jothi Ramalingam ; Thajuddin, Nooruddin ; Ayyamperumal, Ramamoorthy |
Zeitschrift: | Environmental Research, Jg. 251 (2024-06-20), S. N.PAG |
Veröffentlichung: | 2024 |
Medientyp: | academicJournal |
ISSN: | 0013-9351 (print) |
DOI: | 10.1016/j.envres.2024.118698 |
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