Determination of the heat shock protein 90 inhibitor 17-dimethylaminoethylamino-17-demethoxygeldanamycin in plasma by liquid chromatography–electrospray mass spectrometry
In: Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences, Jg. 830 (2006-01-02), Heft 1, S. 35-40
academicJournal
Zugriff:
Abstract: A rapid method was developed for the quantitative determination of the novel heat shock protein 90 inhibitor, 17-dimethylaminoethylamino-17-demethoxygeldanamycin (17-DMAG; NSC707545), in human plasma. Calibration curves were constructed, and were analyzed using a weight factor proportional to the nominal concentration. Sample pretreatment involved a one-step extraction with ethyl acetate of 0.5-ml samples. The analysis was performed in the range of 1–100ng/ml on a column (75mm×2.1mm internal diameter with 3.5μm C18 particle size), using 55% methanol in water containing formic acid as the mobile phase. The column effluent was monitored by mass spectrometry with positive electrospray ionization. The values for precision and accuracy were always <8% and <10% relative error, respectively. The method was successfully applied to examine the pharmacokinetics of 17-DMAG in a cancer patient. [Copyright &y& Elsevier]
| Titel: |
Determination of the heat shock protein 90 inhibitor 17-dimethylaminoethylamino-17-demethoxygeldanamycin in plasma by liquid chromatography–electrospray mass spectrometry
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| Autor/in / Beteiligte Person: | Hwang, Kyunghwa ; Scripture, Charity D. ; Gutierrez, Martin ; Kummar, Shivaani ; Figg, William D. ; Sparreboom, Alex |
| Zeitschrift: | Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences, Jg. 830 (2006-01-02), Heft 1, S. 35-40 |
| Veröffentlichung: | 2006 |
| Medientyp: | academicJournal |
| ISSN: | 1570-0232 (print) |
| DOI: | 10.1016/j.jchromb.2005.10.019 |
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