Electrochemical Aptasensor for Endocrine Disrupting 17β-Estradiol Based on a Poly(3,4-ethylenedioxylthiopene)-Gold Nanocomposite Platform.
In: Sensors (14248220), Jg. 10 (2010-11-01), Heft 11, S. 9872-9890
Online
academicJournal
Zugriff:
A simple and highly sensitive electrochemical DNA aptasensor with high affinity for endocrine disrupting 17β-estradiol, was developed. Poly(3,4-ethylenedioxylthiophene) (PEDOT) doped with gold nanoparticles (AuNPs) was electrochemically synthesized and employed for the immobilization of biotinylated aptamer towards the detection of the target. The diffusion coefficient of the nanocomposite was 6.50 x 10-7 cm² s-1, which showed that the nanocomposite was highly conducting. Electrochemical impedance investigation also revealed the catalytic properties of the nanocomposite with an exchange current value of 2.16 x 10-4 A, compared to 2.14 x 10-5 A obtained for the bare electrode. Streptavidin was covalently attached to the platform using carbodiimide chemistry and the aptamer immobilized via streptavidin-biotin interaction. The electrochemical signal generated from the aptamer-target molecule interaction was monitored electrochemically using cyclic voltammetry and square wave voltammetry in the presence of [Fe(CN)6]-3/-4 as a redox probe. The signal observed shows a current decrease due to interference of the bound 17β-estradiol. The current drop was proportional to the concentration of 17β-estradiol. The PEDOT/AuNP platform exhibited high electroactivity, with increased peak current. The platform was found suitable for the immobilization of the DNAaptamer. The aptasensor was able to distinguish 17β-estradiol from structurally similar endocrine disrupting chemicals denoting its specificity to 17β-estradiol. The detectable concentration range of the 17β-estradiol was 0.1 nM-100 nM, with a detection limit of 0.02 nM. [ABSTRACT FROM AUTHOR]
Titel: |
Electrochemical Aptasensor for Endocrine Disrupting 17β-Estradiol Based on a Poly(3,4-ethylenedioxylthiopene)-Gold Nanocomposite Platform.
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Autor/in / Beteiligte Person: | Olowu, Rasaq A. ; Arotiba, Omotayo ; Mailu, Stephen N. |
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Zeitschrift: | Sensors (14248220), Jg. 10 (2010-11-01), Heft 11, S. 9872-9890 |
Veröffentlichung: | 2010 |
Medientyp: | academicJournal |
ISSN: | 1424-8220 (print) |
DOI: | 10.3390/s101109872 |
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