Induction of gene expression in sheepshead minnows (Cyprinodon variegatus) treated with 17beta-estradiol, diethylstilbestrol, or ethinylestradiol: the use of mRNA fingerprints as an indicator of gene regulation.
In: General and comparative endocrinology, Jg. 121 (2001-03-01), Heft 3, S. 250-60
Online
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Zugriff:
The recent interest in hormonally active environmental contaminants has sparked a drive to find sensitive methods to measure their effects on wildlife. A molecular-based assay has been developed to measure the induction of gene expression in sheepshead minnows (Cyprinodon variegatus) exposed in vivo to the natural and pharmaceutical estrogens 17beta-estradiol, ethinylestradiol, and diethylstilbestrol. This method used differential display reverse transcriptase polymerase chain reaction assays to compare the expression of individual mRNAs from control and estrogen-exposed fish. Forty-eight differentially expressed cDNAs were isolated by this method, including cDNAs for vitelline envelope proteins and vitellogenin. The mRNA expression patterns for fish injected with a pharmacological dose of estradiol (5 mg/kg) were identical to those obtained in fish receiving constant aqueous exposure to 212 ng estradiol/liter. Further, the cDNA "fingerprint" pattern observed in the estradiol-treated fish also matched that obtained in fish receiving continuous-flow aqueous exposures to 192 ng ethinyl estradiol/liter and a nominal concentration of 200 ng diethylstilbestrol/liter. The results demonstrate a characteristic expression pattern for genes upregulated by exposure to a variety of natural and anthropogenic estrogens and suggest this approach may be valuable to examine the potential effects of environmental contaminants on other endocrine-mediated pathways of reproduction, growth, and development.
(Copyright 2001 Academic Press.)
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Induction of gene expression in sheepshead minnows (Cyprinodon variegatus) treated with 17beta-estradiol, diethylstilbestrol, or ethinylestradiol: the use of mRNA fingerprints as an indicator of gene regulation.
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Autor/in / Beteiligte Person: | Denslow, ND ; Bowman, CJ ; Ferguson, RJ ; Lee, HS ; Hemmer, MJ ; Folmar, LC |
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Zeitschrift: | General and comparative endocrinology, Jg. 121 (2001-03-01), Heft 3, S. 250-60 |
Veröffentlichung: | New York, NY : Academic Press ; <i>Original Publication</i>: New York., 2001 |
Medientyp: | academicJournal |
ISSN: | 0016-6480 (print) |
DOI: | 10.1006/gcen.2001.7605 |
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