Generation of Helicobacter pylori ghosts by PhiX protein E-mediated inactivation and their evaluation as vaccine candidates.
In: Infection and immunity, Jg. 71 (2003), Heft 1, S. 109-16
Online
academicJournal
Zugriff:
Bacterial ghosts are empty cell envelopes, which may be generated by the controlled expression of the PhiX174 lysis gene E in gram-negative bacteria to obtain vaccine candidates. We describe here the application of this technology to Helicobacter pylori. The lysis gene cassette was cloned into an Escherichia coli-Helicobacter pylori shuttle vector and introduced into an H. pylori recipient strain by bacterial conjugation. Temperature induction of the lysis gene cassette revealed a quantitative killing of the H. pylori culture without induction of lysis-resistant bacteria. Biochemical and transmission electron microscopic studies identified structurally intact H. pylori. Prophylactic oral vaccination experiments using these H. pylori ghosts in the BALB/c mouse model showed a significant reduction of the bacterial load in the ghost group, as measured by a quantitative bacterial reisolation procedure. Ten of 10 and 5 of 10 mice were protected, respectively, without the use of a mucosal adjuvant. Coadministration of ghosts with cholera toxin as mucosal adjuvant resulted in a complete protection of 10 of 10 and 8 of 8 mice against H. pylori challenge, with three animals showing a sterile immunity.
Titel: |
Generation of Helicobacter pylori ghosts by PhiX protein E-mediated inactivation and their evaluation as vaccine candidates.
|
---|---|
Autor/in / Beteiligte Person: | Panthel, K ; Jechlinger, W ; Matis, A ; Rohde, M ; Szostak, M ; Lubitz, W ; Haas, R |
Link: | |
Zeitschrift: | Infection and immunity, Jg. 71 (2003), Heft 1, S. 109-16 |
Veröffentlichung: | Washington, DC : American Society For Microbiology ; <i>Original Publication</i>: [Bethesda, Md.] American Society for Microbiology., 2003 |
Medientyp: | academicJournal |
ISSN: | 0019-9567 (print) |
DOI: | 10.1128/IAI.71.1.109-116.2003 |
Schlagwort: |
|
Sonstiges: |
|