17beta-estradiol up-regulates prostacyclin production in cultured human uterine myometrial cells via augmentation of both cyclooxygenase-1 and prostacyclin synthase expression.
In: Journal of the Society for Gynecologic Investigation, Jg. 11 (2004-10-01), Heft 7, S. 457-64
Online
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Zugriff:
Objective: To investigate whether 17beta-estradiol elevates prostacyclin (PGI(2)) production in human myometrial cells in the middle of gestation.
Methods: The concentration of 6-keto-PGF(1alpha), a stable metabolite of PGI(2), in the culture medium was assessed using enzyme-linked immunosorbent assay (ELISA). Western blot analysis and quantitative reverse-transcriptase polymerase chain reaction (RT-PCR) using TaqMan (Applied Biosystems, Foster City, CA) technology were performed to evaluate the expression of cytosolic phopholipase A(2) (cPLA(2)), cyclooxygenase-1 (COX-1), COX-2, and prostacyclin synthase (PGIS) in cultured human myometrial cells prepared from second trimester pregnant women (n = 3) after stimulation with 17beta-estradiol.
Results: Treatment with 17beta-estradiol (4-400 nM) dose-dependently elevated PGI(2) secretion from cultured human myometrial cells. Western blot analysis detected cPLA(2) and COX-1 and PGIS protein expression in the cultured human myometrial cells; however, COX-2 protein expression was below the detection sensitivity. Stimulation with 40-nM 17beta-estradiol significantly up-regulated protein and mRNA expression of both COX-1 and PGIS.
Conclusion: 17beta-Estradiol from placenta may contribute to the augmentation of PGI(2) production in the human myometrium in the middle of gestation via up-regulation of both COX-1 and PGIS expression.
Titel: |
17beta-estradiol up-regulates prostacyclin production in cultured human uterine myometrial cells via augmentation of both cyclooxygenase-1 and prostacyclin synthase expression.
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Autor/in / Beteiligte Person: | Korita, D ; Itoh, H ; Sagawa, N ; Yura, S ; Yoshida, M ; Kakui, K ; Takemura, M ; Fujii, S |
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Zeitschrift: | Journal of the Society for Gynecologic Investigation, Jg. 11 (2004-10-01), Heft 7, S. 457-64 |
Veröffentlichung: | New York, NY : Elsevier, c1994-, 2004 |
Medientyp: | academicJournal |
ISSN: | 1071-5576 (print) |
DOI: | 10.1016/j.jsgi.2004.04.002 |
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