Spectral unmixing of flavin autofluorescence components in cardiac myocytes.
In: Biophysical journal, Jg. 89 (2005-12-01), Heft 6, S. L55-7
Online
academicJournal
Zugriff:
We applied linear unmixing approach to reveal individual components of intrinsic flavin fluorescence signal recorded in living cardiac cells by spectrally resolved confocal microscopy. Responses of whole-cell autofluorescence to modulators of cell metabolism and respiration were used as a tool of separation of its components; their spectral profiles, estimated by principal component analysis, correspond to free FAD and FAD bound to different enzymes of electron transport chain.
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Spectral unmixing of flavin autofluorescence components in cardiac myocytes.
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Autor/in / Beteiligte Person: | Chorvat D Jr ; Kirchnerova, J ; Cagalinec, M ; Smolka, J ; Mateasik, A ; Chorvatova, A |
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Zeitschrift: | Biophysical journal, Jg. 89 (2005-12-01), Heft 6, S. L55-7 |
Veröffentlichung: | Cambridge, MA : Cell Press ; <i>Original Publication</i>: New York, Published by Rockefeller University Press [etc.] for the Biophysical Society., 2005 |
Medientyp: | academicJournal |
ISSN: | 0006-3495 (print) |
DOI: | 10.1529/biophysj.105.073866 |
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