Molecular cloning and characterization of an alpha-amylase occurring in the pulp of ripening bananas and its expression in Pichia pastoris.
In: Journal of agricultural and food chemistry, Jg. 54 (2006-10-18), Heft 21, S. 8222-8
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Zugriff:
Alpha-amylases (EC 3.2.1.1) are glycosyl hydrolases with endoglycolytic activity on the alpha-1,4-d-glucosidic linkages in starch. In bananas, the mobilization of starch accounts for sugar accumulation during ripening, and among several hydrolytic enzymes, alpha-amylase is the only enzyme argued to be able to attack the intact granules, indicating a pivotal role for this enzyme. A 1953 bp full-length banana alpha-amylase cDNA (MAmy), encoded for a sequence of 416 amino acids, was cloned and used for heterologous expression in Pichia pastoris. The cloned MAmy presented the highly conserved motifs common to alpha-amylases, and the amylolytic activity of the extracts from yeast transformed with MAmy demonstrated that it encodes for a functional alpha-amylase, suggesting a putative role for this gene in starch degradation during fruit ripening.
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Molecular cloning and characterization of an alpha-amylase occurring in the pulp of ripening bananas and its expression in Pichia pastoris.
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Autor/in / Beteiligte Person: | Junior, AV ; do Nascimento JR ; Lajolo, FM |
Zeitschrift: | Journal of agricultural and food chemistry, Jg. 54 (2006-10-18), Heft 21, S. 8222-8 |
Veröffentlichung: | Washington, American Chemical Society., 2006 |
Medientyp: | academicJournal |
ISSN: | 0021-8561 (print) |
DOI: | 10.1021/jf060805b |
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