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A T-cell-stimulating, membrane-located 17-kDa protein of the live vaccine strain Francisella tularensis LVS has previously been cloned and sequenced. In the present study, it is shown to be a lipoprotein. When F. tularensis was grown in the presence of [3H]palmitate, several proteins of the organism, including a 17-kDa protein, were radiolabeled. The labeled 17-kDa protein was found by Western blot (immunoblot) analysis to be identical to the cloned protein. It was located in the detergent phase after partitioning with the nonionic detergent Triton X-114, thereby behaving like a hydrophobic integral membrane protein. The protein was predominantly hydrophilic and contained no putative transmembrane domain. The presence of fatty acids is therefore the probable explanation of the membrane location of the 17-kDa protein. The amino acid sequence of the 17-kDa protein contains the tetrapeptide Leu-Ala-Ser-Cys, which is a recognition sequence of the lipoprotein signal peptidase. Globomycin, a specific inhibitor of the peptidase, inhibited maturation of the 17-kDa lipoprotein. The protein incorporated [3H]palmitate also when expressed by Escherichia coli. The 17-kDa lipoprotein was recognized not only by T cells but also by serum antibodies of F. tularensis-primed individuals.

Titel:	The T-cell-stimulating 17-kilodalton protein of Francisella tularensis LVS is a lipoprotein.
Autor/in / Beteiligte Person:	Sjöstedt, A ; Tärnvik, A ; Sandström, G
Link:	Full Text Finder (Volltext)
Zeitschrift:	Infection and immunity, Jg. 59 (1991-09-01), Heft 9, S. 3163-8
Veröffentlichung:	Washington, DC : American Society For Microbiology ; <i>Original Publication</i>: [Bethesda, Md.] American Society for Microbiology., 1991
Medientyp:	academicJournal
ISSN:	0019-9567 (print)
DOI:	10.1128/iai.59.9.3163-3168.1991
Schlagwort:	Adolescent Adult Amino Acid Sequence Antibodies, Bacterial immunology Bacterial Proteins genetics Bacterial Proteins immunology Blotting, Western Electrophoresis, Polyacrylamide Gel Fatty Acids metabolism Francisella tularensis drug effects Humans Lipoproteins genetics Lipoproteins immunology Middle Aged Molecular Sequence Data Molecular Weight Palmitates metabolism Plasmids Recombinant Proteins immunology Tularemia immunology Bacterial Proteins isolation & purification Francisella tularensis immunology Lipoproteins isolation & purification Lymphocyte Activation T-Lymphocytes immunology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Infect Immun] 1991 Sep; Vol. 59 (9), pp. 3163-8. MeSH Terms: Lymphocyte Activation* ; Bacterial Proteins / isolation & purification ; Francisella tularensis / immunology ; Lipoproteins / isolation & purification ; T-Lymphocytes / immunology ; Adolescent ; Adult ; Amino Acid Sequence ; Antibodies, Bacterial / immunology ; Bacterial Proteins / genetics ; Bacterial Proteins / immunology ; Blotting, Western ; Electrophoresis, Polyacrylamide Gel ; Fatty Acids / metabolism ; Francisella tularensis / drug effects ; Humans ; Lipoproteins / genetics ; Lipoproteins / immunology ; Middle Aged ; Molecular Sequence Data ; Molecular Weight ; Palmitates / metabolism ; Plasmids ; Recombinant Proteins / immunology ; Tularemia / immunology References: Eur J Biochem. 1973 Apr;34(2):284-96. (PMID: 4575979) ; Nature. 1970 Aug 15;227(5259):680-5. (PMID: 5432063) ; J Bacteriol. 1951 Sep;62(3):293-300. (PMID: 14888646) ; Eur J Biochem. 1987 Feb 16;163(1):73-7. (PMID: 3545827) ; Curr Top Microbiol Immunol. 1986;125:127-57. (PMID: 3527576) ; Microb Pathog. 1989 Jun;6(6):403-14. (PMID: 2475738) ; Immunology. 1986 Jun;58(2):245-50. (PMID: 2423444) ; Infect Immun. 1989 Sep;57(9):2872-7. (PMID: 2668191) ; Microb Pathog. 1989 Dec;7(6):411-9. (PMID: 2622330) ; Nature. 1989 Nov 30;342(6249):561-4. (PMID: 2586628) ; Infect Immun. 1988 Feb;56(2):490-8. (PMID: 3276627) ; Infect Immun. 1988 May;56(5):1194-202. (PMID: 3356465) ; J Immunol. 1986 Oct 15;137(8):2676-81. (PMID: 3531339) ; J Clin Microbiol. 1987 Apr;25(4):641-4. (PMID: 3494744) ; J Immunol. 1990 Jul 1;145(1):311-7. (PMID: 1694206) ; Infect Immun. 1990 Aug;58(8):2664-8. (PMID: 1695207) ; J Clin Microbiol. 1990 Jan;28(1):43-8. (PMID: 1688874) ; Infect Immun. 1990 Apr;58(4):983-91. (PMID: 2318538) ; Proc Natl Acad Sci U S A. 1990 Mar;87(5):1735-9. (PMID: 2308932) ; Proc Natl Acad Sci U S A. 1982 Apr;79(7):2255-9. (PMID: 7048314) ; J Biol Chem. 1981 Mar 25;256(6):3125-9. (PMID: 7009608) ; Infect Immun. 1984 Jul;45(1):101-6. (PMID: 6203835) ; Infect Immun. 1982 Nov;38(2):668-72. (PMID: 6183218) ; J Biol Chem. 1981 Feb 25;256(4):1604-7. (PMID: 6257680) ; Infect Immun. 1983 Feb;39(2):715-20. (PMID: 6832815) ; J Mol Biol. 1982 May 5;157(1):105-32. (PMID: 7108955) ; J Antibiot (Tokyo). 1978 Nov;31(11):1203-5. (PMID: 721715) ; Biochim Biophys Acta. 1975 Oct 31;415(3):335-77. (PMID: 52377) ; Can J Biochem Physiol. 1959 Aug;37(8):911-7. (PMID: 13671378) Substance Nomenclature: 0 (Antibodies, Bacterial) ; 0 (Bacterial Proteins) ; 0 (Fatty Acids) ; 0 (Lipoproteins) ; 0 (Palmitates) ; 0 (Recombinant Proteins) Entry Date(s): Date Created: 19910901 Date Completed: 19911002 Latest Revision: 20210526 Update Code: 20231215 PubMed Central ID: PMC258148

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The T-cell-stimulating 17-kilodalton protein of Francisella tularensis LVS is a lipoprotein.