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Background: An important question in dengue pathogenesis is the identity of immune cells involved in the control of dengue virus infection at the site of the mosquito bite. There is evidence that infection of immature myeloid dendritic cells plays a crucial role in dengue pathogenesis and that the interaction of the viral envelope E glycoprotein with CD209/DC-SIGN is a key element for their productive infection. Dermal macrophages express CD209, yet little is known about their role in dengue virus infection.

Methods and Findings: Here, we showed that dermal macrophages bound recombinant envelope E glycoprotein fused to green fluorescent protein. Because dermal macrophages stain for IL-10 in situ, we generated dermal-type macrophages from monocytes in the presence of IL-10 to study their infection by dengue virus. The macrophages were able to internalize the virus, but progeny virus production was undetectable in the infected cells. In addition, no IFN-alpha was produced in response to the virus. The inability of dengue virus to grow in the macrophages was attributable to accumulation of internalized virus particles into poorly-acidified phagosomes.

Conclusions: Aborting infection by viral sequestration in early phagosomes would present a novel means to curb infection of enveloped virus and may constitute a prime defense system to prevent dengue virus spread shortly after the bite of the infected mosquito.

Titel:	Dermal-type macrophages expressing CD209/DC-SIGN show inherent resistance to dengue virus growth.
Autor/in / Beteiligte Person:	Kwan, WH ; Navarro-Sanchez, E ; Dumortier, H ; Decossas, M ; Vachon, H ; dos Santos FB ; Fridman, HW ; Rey, FA ; Harris, E ; Despres, P ; Mueller, CG
Link:	Full Text Finder (Volltext)
Zeitschrift:	PLoS neglected tropical diseases, Jg. 2 (2008-10-01), Heft 10, S. e311
Veröffentlichung:	San Francisco, CA : Public Library of Science, 2008
Medientyp:	academicJournal
ISSN:	1935-2735 (electronic)
DOI:	10.1371/journal.pntd.0000311
Schlagwort:	Cell Adhesion Molecules genetics Cell Adhesion Molecules metabolism Cell Line Cells, Cultured Dengue genetics Dengue metabolism Dengue virology Dengue Virus immunology Dengue Virus metabolism Humans Lectins, C-Type genetics Lectins, C-Type metabolism Macrophages metabolism Macrophages virology Protein Binding Receptors, Cell Surface genetics Receptors, Cell Surface metabolism Skin metabolism Skin virology Viral Envelope Proteins immunology Viral Envelope Proteins metabolism Cell Adhesion Molecules immunology Dengue immunology Dengue Virus growth & development Gene Expression Lectins, C-Type immunology Macrophages immunology Receptors, Cell Surface immunology Skin immunology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [PLoS Negl Trop Dis] 2008 Oct 01; Vol. 2 (10), pp. e311. <i>Date of Electronic Publication: </i>2008 Oct 01. MeSH Terms: Gene Expression* ; Cell Adhesion Molecules / immunology ; Dengue / immunology ; Dengue Virus / growth & development ; Lectins, C-Type / immunology ; Macrophages / immunology ; Receptors, Cell Surface / immunology ; Skin / *immunology ; Cell Adhesion Molecules / genetics ; Cell Adhesion Molecules / metabolism ; Cell Line ; Cells, Cultured ; Dengue / genetics ; Dengue / metabolism ; Dengue / virology ; Dengue Virus / immunology ; Dengue Virus / metabolism ; Humans ; Lectins, C-Type / genetics ; Lectins, C-Type / metabolism ; Macrophages / metabolism ; Macrophages / virology ; Protein Binding ; Receptors, Cell Surface / genetics ; Receptors, Cell Surface / metabolism ; Skin / metabolism ; Skin / virology ; Viral Envelope Proteins / immunology ; Viral Envelope Proteins / metabolism References: Science. 1988 Jan 29;239(4839):476-81. (PMID: 3277268) ; EMBO Rep. 2003 Jul;4(7):723-8. (PMID: 12783086) ; J Invest Dermatol. 1998 Jul;111(1):31-8. (PMID: 9665383) ; J Virol. 2005 Jun;79(12):7291-9. (PMID: 15919883) ; Nat Med. 2005 Jun;11(6):653-60. (PMID: 15880118) ; J Immunol. 2005 Oct 1;175(7):4265-73. (PMID: 16177066) ; J Immunol. 2005 Dec 15;175(12):7905-15. (PMID: 16339526) ; J Gen Virol. 2006 Oct;87(Pt 10):2755-66. (PMID: 16963734) ; Immunol Cell Biol. 2007 Jul;85(5):363-9. (PMID: 17342064) ; J Clin Invest. 2007 Sep;117(9):2382-5. (PMID: 17786233) ; J Clin Invest. 2007 Sep;117(9):2517-25. (PMID: 17786242) ; Int Immunol. 2007 Nov;19(11):1271-9. (PMID: 17804688) ; PLoS Pathog. 2008 Feb 8;4(2):e17. (PMID: 18266465) ; Nat Med. 2000 Jul;6(7):816-20. (PMID: 10888933) ; J Immunol. 2001 Feb 1;166(3):1499-506. (PMID: 11160189) ; J Immunol. 2002 Mar 15;168(6):2634-43. (PMID: 11884427) ; Immunology. 2002 Aug;106(4):537-48. (PMID: 12153517) ; Nat Immunol. 2002 Oct;3(10):975-83. (PMID: 12352970) ; J Exp Med. 2003 Apr 7;197(7):823-9. (PMID: 12682107) ; J Virol. 1998 Jan;72(1):823-9. (PMID: 9420294) Substance Nomenclature: 0 (Cell Adhesion Molecules) ; 0 (DC-specific ICAM-3 grabbing nonintegrin) ; 0 (Lectins, C-Type) ; 0 (Receptors, Cell Surface) ; 0 (Viral Envelope Proteins) Entry Date(s): Date Created: 20081002 Date Completed: 20100315 Latest Revision: 20181113 Update Code: 20240513 PubMed Central ID: PMC2553280

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Dermal-type macrophages expressing CD209/DC-SIGN show inherent resistance to dengue virus growth.