Knockdown of SKN-1 and the Wnt effector TCF/POP-1 reveals differences in endomesoderm specification in C. briggsae as compared with C. elegans.
In: Developmental biology, Jg. 325 (2009), Heft 1, S. 296-306
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Zugriff:
In the nematode, C. elegans, the bZIP/homeodomain transcription factor SKN-1 and the Wnt effector TCF/POP-1 are central to the maternal specification of the endomesoderm prior to gastrulation. The 8-cell stage blastomere MS is primarily a mesodermal precursor, giving rise to cells of the pharynx and body muscle among others, while its sister E clonally generates the entire endoderm (gut). In C. elegans, loss of SKN-1 results in the absence of MS-derived tissues all of the time, and loss of gut most of the time, while loss of POP-1 results in a mis-specification of MS as an E-like cell, resulting in ectopic gut. We show that in C. briggsae, RNAi of skn-1 results in a stronger E defect but no apparent MS defect, while RNAi of pop-1 results in loss of gut and an apparent E to MS transformation, the opposite of the pop-1 knockdown phenotype seen in C. elegans. The difference in pop-1(-) phenotypes correlates with changes in how the endogenous endoderm-specifying end genes are regulated by POP-1 in the two species. Our results suggest that integration of Wnt-dependent and Wnt-independent cell fate specification pathways within the Caenorhabditis genus can occur in different ways.
Titel: |
Knockdown of SKN-1 and the Wnt effector TCF/POP-1 reveals differences in endomesoderm specification in C. briggsae as compared with C. elegans.
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Autor/in / Beteiligte Person: | Lin, KT ; Broitman-Maduro, G ; Hung, WW ; Cervantes, S ; Maduro, MF |
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Zeitschrift: | Developmental biology, Jg. 325 (2009), Heft 1, S. 296-306 |
Veröffentlichung: | San Diego, CA : Elsevier ; <i>Original Publication</i>: New York., 2009 |
Medientyp: | academicJournal |
ISSN: | 1095-564X (electronic) |
DOI: | 10.1016/j.ydbio.2008.10.001 |
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