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Objective: To compare levels of middle ear (ME) MUC5AC expression in patients with otitis media (OM) with patients without OM. Mucin gene 5AC has been identified as a major secretory mucin in the ME and is fundamentally important in the development of ME mucoid effusions, hearing loss and also provides ME mucosal protection and bacterial clearance.

Design: Case control.

Setting: Tertiary, academic, pediatric otolaryngology practice.

Patients: Patients 9 months to 7 years old undergoing routine tympanostomy tube (TT) insertion for recurrent otitis media (RecOM) or chronic otitis media with effusion (COME) were compared with control patients without a history of OM undergoing cochlear implantation.

Methods: During routine TT placement or cochlear implantation, a 1-mm biopsy sample of the ME epithelium was obtained. RNA was extracted, and real-time reverse transcriptase-polymerase chain reaction was used to quantify levels of MUC5AC expression.

Results: Twenty-three patients with OM (12 with RecOM and with 11 COME) were evaluated using 5 controls. Mean age was not different between groups. In the RecOM group, mean expression of MUC5AC was 25.92 times greater than in controls. In the COME group, the mean expression was 155.40 times greater than in controls.

Conclusions: Levels of MUC5AC expression in the human ME are significantly increased in patients with RecOM and COME compared with controls. This study demonstrates MUC5AC gene changes in patients with OM and highlights the need for greater understanding of the molecular responses in OM; particularly that of mucin. A thorough exploration of these factors will provide opportunities to develop novel interventions for the extremely common problem of OM.

Titel:	MUC5AC expression in human middle ear epithelium of patients with otitis media.
Autor/in / Beteiligte Person:	Kerschner, JE ; Tripathi, S ; Khampang, P ; Papsin, BC
Zeitschrift:	Archives of otolaryngology--head & neck surgery, Jg. 136 (2010-08-01), Heft 8, S. 819-24
Veröffentlichung:	[Chicago, IL] : American Medical Association, [c1986-, 2010
Medientyp:	academicJournal
ISSN:	1538-361X (electronic)
DOI:	10.1001/archoto.2010.123
Schlagwort:	Child Child, Preschool Chronic Disease Cochlear Implantation Ear, Middle pathology Enzyme-Linked Immunosorbent Assay Epithelium pathology Female Humans Immunoenzyme Techniques Infant Male Middle Ear Ventilation Otitis Media pathology Otitis Media with Effusion pathology Recurrence Reference Values Reverse Transcriptase Polymerase Chain Reaction Ear, Middle metabolism Epithelium metabolism Gene Expression genetics Mucin 5AC genetics Otitis Media genetics Otitis Media with Effusion genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Language: English [Arch Otolaryngol Head Neck Surg] 2010 Aug; Vol. 136 (8), pp. 819-24. MeSH Terms: Ear, Middle / metabolism ; Epithelium / metabolism ; Gene Expression / genetics ; Mucin 5AC / genetics ; Otitis Media / genetics ; Otitis Media with Effusion / genetics ; Child ; Child, Preschool ; Chronic Disease ; Cochlear Implantation ; Ear, Middle / pathology ; Enzyme-Linked Immunosorbent Assay ; Epithelium / pathology ; Female ; Humans ; Immunoenzyme Techniques ; Infant ; Male ; Middle Ear Ventilation ; Otitis Media / pathology ; Otitis Media with Effusion / pathology ; Recurrence ; Reference Values ; Reverse Transcriptase Polymerase Chain Reaction References: J Assoc Res Otolaryngol. 2003 Sep;4(3):384-93. (PMID: 14690056) ; Biochem Biophys Res Commun. 2008 Dec 19;377(3):763-8. (PMID: 18957283) ; Laryngoscope. 2007 Sep;117(9):1666-76. (PMID: 17667140) ; Laryngoscope. 1994 Aug;104(8 Pt 1):989-95. (PMID: 8052085) ; Arch Otolaryngol Head Neck Surg. 1995 Aug;121(8):865-9. (PMID: 7619411) ; Laryngoscope. 2002 Jan;112(1):152-5. (PMID: 11802055) ; Arch Otolaryngol Head Neck Surg. 2008 Jan;134(1):39-44. (PMID: 18209134) ; Biochem Biophys Res Commun. 2008 Jan 25;365(4):795-800. (PMID: 18037371) ; Am J Respir Cell Mol Biol. 2006 Jun;34(6):661-5. (PMID: 16456183) ; Cytokine. 2000 Nov;12(11):1732-6. (PMID: 11052828) ; Ann Otol Rhinol Laryngol. 1998 Jan;107(1):28-33. (PMID: 9439385) ; Cytokine. 2004 Apr 7;26(1):30-6. (PMID: 15016409) ; Laryngoscope. 2006 Mar;116(3):365-9. (PMID: 16540890) ; Am J Physiol Lung Cell Mol Physiol. 2001 Jun;280(6):L1157-67. (PMID: 11350794) ; Eur Cytokine Netw. 2001 Mar;12(1):119-25. (PMID: 11282555) ; Methods. 2001 Dec;25(4):402-8. (PMID: 11846609) ; Arch Otolaryngol Head Neck Surg. 2004 Oct;130(10):1163-7. (PMID: 15492162) Grant Information: R01 DC007903 United States DC NIDCD NIH HHS; R01 DC007903-03 United States DC NIDCD NIH HHS; DC007903 United States DC NIDCD NIH HHS Substance Nomenclature: 0 (MUC5AC protein, human) ; 0 (Mucin 5AC) Entry Date(s): Date Created: 20100818 Date Completed: 20100915 Latest Revision: 20211020 Update Code: 20240513 PubMed Central ID: PMC2924762

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MUC5AC expression in human middle ear epithelium of patients with otitis media.