Interleukin-10 repletion suppresses pro-inflammatory cytokines and decreases liver pathology without altering viral replication in murine cytomegalovirus (MCMV)-infected IL-10 knockout mice.
In: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Jg. 60 (2011-03-01), Heft 3, S. 233-43
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Zugriff:
Objective and Design: To determine the role of interleukin-10 (IL-10) in protecting against the deleterious pro-inflammatory cytokine response to murine cytomegalovirus (MCMV), we studied the impact of IL-10 repletion in MCMV-infected IL-10 knockout (KO) mice.
Materials and Methods: IL-10 KO mice were infected with a sub-lethal dose of MCMV and treated daily with 5 μg of mouse recombinant IL-10 (mrIL-10). Cytokine transcription, viral load, cytokine expression and liver histopathology were assessed in IL-10 treated and untreated mice.
Results: mrIL-10 repletion suppressed the exaggerated pro-inflammatory cytokine response observed in IL-10 KO mice (vs. control) both systemically and at the organ level, without affecting viral load. Levels of IFN-γ and TNF-α mRNA in livers of treated mice were ~50-70-fold lower than in untreated mice at day 5 post-infection (p ≤ 0.05). In spleens and sera, levels of IFN-γ and IL-6 were significantly lower in treated mice than in untreated mice at day 5-7 post-infection (p ≤ 0.05). IL-10 blunting of cytokine responses was accompanied by attenuation of inflammation in livers of treated mice.
Conclusions: Repletion of IL-10 modulates the exaggerated pro-inflammatory cytokine responses that characterize IL-10 KO mice and protects against liver damage without altering viral load. IL-10 may be useful to control dysregulated pro-inflammatory cytokines responses during CMV infection.
Titel: |
Interleukin-10 repletion suppresses pro-inflammatory cytokines and decreases liver pathology without altering viral replication in murine cytomegalovirus (MCMV)-infected IL-10 knockout mice.
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Autor/in / Beteiligte Person: | Tang-Feldman, YJ ; Lochhead, GR ; Lochhead, SR ; Yu, C ; Pomeroy, C |
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Zeitschrift: | Inflammation research : official journal of the European Histamine Research Society ... [et al.], Jg. 60 (2011-03-01), Heft 3, S. 233-43 |
Veröffentlichung: | Basel, Switzerland : Birkhäuser, c1995-, 2011 |
Medientyp: | academicJournal |
ISSN: | 1420-908X (electronic) |
DOI: | 10.1007/s00011-010-0259-4 |
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