Einzeltreffer — DigiBib

In order to investigate regulation and redundancy within the sloppy paired (slp) locus, we analyzed 30 kilobases of DNA encompassing the tandem, coordinately regulated slp1 and slp2 transcription units. We found a remarkable array of stripe enhancers with overlapping activities surrounding the slp1 transcription unit, and, unexpectedly, glial cell enhancers surrounding slp2. The slp stripe regulatory region generates 7 stripes at blastoderm, and later 14 stripes that persist throughout embryogenesis. Phylogenetic analysis among drosophilids suggests that the multiplicity of stripe enhancers did not evolve through recent duplication. Most of the direct integration among cis-regulatory modules appears to be simply additive, with one notable exception. Despite the apparent redundancy among stripe enhancers, transgenic rescue suggests that most are required for full function, to maintain wingless expression and parasegment boundaries throughout embryogenesis. Transgenic rescue also reveals indirect positive autoregulation by the 7 early stripes, without which alternate stripes within the 14-stripe pattern are lost, leading to embryos with a pair-rule phenotype.

Titel:	Regulation of a duplicated locus: Drosophila sloppy paired is replete with functionally overlapping enhancers.
Autor/in / Beteiligte Person:	Fujioka, M ; Jaynes, JB
Link:	Full Text Finder (Volltext)
Zeitschrift:	Developmental biology, Jg. 362 (2012-02-15), Heft 2, S. 309-19
Veröffentlichung:	San Diego, CA : Elsevier ; <i>Original Publication</i>: New York., 2012
Medientyp:	academicJournal
ISSN:	1095-564X (electronic)
DOI:	10.1016/j.ydbio.2011.12.001
Schlagwort:	Animals Base Sequence Computational Biology Conserved Sequence genetics Enhancer Elements, Genetic physiology In Situ Hybridization Molecular Sequence Data Neuroglia metabolism Phylogeny Sequence Analysis, DNA Drosophila Proteins genetics Drosophila melanogaster genetics Enhancer Elements, Genetic genetics Evolution, Molecular Gene Expression Regulation, Developmental genetics Genes, Duplicate genetics Transcription Factors genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Language: English [Dev Biol] 2012 Feb 15; Vol. 362 (2), pp. 309-19. <i>Date of Electronic Publication: </i>2011 Dec 09. MeSH Terms: Evolution, Molecular* ; Drosophila Proteins / genetics ; Drosophila melanogaster / genetics ; Enhancer Elements, Genetic / genetics ; Gene Expression Regulation, Developmental / genetics ; Genes, Duplicate / genetics ; Transcription Factors / genetics ; Animals ; Base Sequence ; Computational Biology ; Conserved Sequence / genetics ; Enhancer Elements, Genetic / physiology ; In Situ Hybridization ; Molecular Sequence Data ; Neuroglia / metabolism ; Phylogeny ; Sequence Analysis, DNA References: Mol Biol Evol. 1995 May;12(3):391-404. (PMID: 7739381) ; Development. 1987 Sep;101(1):1-22. (PMID: 2896587) ; Dev Biol. 2002 Aug 15;248(2):369-83. (PMID: 12167411) ; Genome Biol. 2002;3(12):RESEARCH0088. (PMID: 12537577) ; Proc Natl Acad Sci U S A. 2005 Oct 11;102(41):14700-5. (PMID: 16203978) ; Genome Biol. 2007;8(5):R75. (PMID: 17490485) ; Development. 1995 Dec;121(12):4371-82. (PMID: 8575337) ; Nature. 2007 Nov 8;450(7167):203-18. (PMID: 17994087) ; Dev Biol. 2010 Jul 1;343(1-2):167-77. (PMID: 20403347) ; Nature. 2010 Jul 22;466(7305):490-3. (PMID: 20512118) ; Dev Biol. 2010 Aug 15;344(2):1048-59. (PMID: 20435028) ; Development. 2003 Feb;130(4):741-51. (PMID: 12506004) ; Development. 2003 Nov;130(22):5385-400. (PMID: 13129849) ; Genetics. 2006 Jun;173(2):769-77. (PMID: 16547094) ; PLoS Biol. 2004 Sep;2(9):E271. (PMID: 15340490) ; Genetics. 2004 Apr;166(4):1775-82. (PMID: 15126397) ; Cell. 1986 Dec 5;47(5):721-34. (PMID: 2877745) ; Curr Top Dev Biol. 2009;88:1-33. (PMID: 19651300) ; Nature. 2011 Mar 24;471(7339):527-31. (PMID: 21430782) ; Curr Biol. 2010 Sep 14;20(17):1562-7. (PMID: 20797865) ; Proc Natl Acad Sci U S A. 2005 Apr 5;102(14):4960-5. (PMID: 15793007) ; Dev Biol. 2004 May 15;269(2):609-22. (PMID: 15110723) ; Mech Dev. 1995 Jun;51(2-3):199-215. (PMID: 7547468) ; Evol Dev. 2005 Nov-Dec;7(6):618-28. (PMID: 16336415) ; Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6324-8. (PMID: 8022780) ; Genes Dev. 1994 Apr 15;8(8):981-94. (PMID: 7926782) ; Science. 2000 Mar 24;287(5461):2271-4. (PMID: 10731150) ; PLoS Biol. 2009 Jan 13;7(1):e13. (PMID: 19143474) ; Genome Biol. 2007;8(7):R145. (PMID: 17645804) ; Science. 1982 Oct 22;218(4570):341-7. (PMID: 6289435) ; Nat Methods. 2009 Jun;6(6):431-4. (PMID: 19465919) ; Nucleic Acids Res. 2008 Jan;36(Database issue):D102-6. (PMID: 18006571) ; Nucleic Acids Res. 2009 Jan;37(Database issue):D555-9. (PMID: 18948289) ; Nature. 1988 Sep 1;335(6185):25-34. (PMID: 2901040) ; Nucleic Acids Res. 2010 Jan;38(Database issue):D105-10. (PMID: 19906716) ; Cell. 1989 May 5;57(3):413-22. (PMID: 2720776) ; Development. 1999 Jun;126(11):2527-38. (PMID: 10226011) ; BMC Genomics. 2008 Aug 01;9:371. (PMID: 18673565) ; Genes Dev. 1994 Apr 15;8(8):899-913. (PMID: 7926775) ; Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3312-7. (PMID: 17360644) ; Development. 2000 Dec;127(24):5497-508. (PMID: 11076769) ; PLoS Biol. 2008 Feb;6(2):e27. (PMID: 18271625) ; EMBO J. 1989 Apr;8(4):1205-12. (PMID: 2743979) ; Development. 2000 Feb;127(3):655-65. (PMID: 10631185) ; Development. 1994 Oct;120(10):2957-66. (PMID: 7607085) ; Development. 2008 Dec;135(24):4131-9. (PMID: 19029043) ; Glia. 2007 Feb;55(3):282-93. (PMID: 17091489) ; Genes Dev. 1992 Jun;6(6):1030-51. (PMID: 1317319) ; Development. 1995 Feb;121(2):317-32. (PMID: 7768175) ; Dev Biol. 2007 Feb 1;302(1):281-94. (PMID: 17054935) Grant Information: R01 GM050231-15 United States GM NIGMS NIH HHS; P30CA56036 United States CA NCI NIH HHS; P30 CA056036 United States CA NCI NIH HHS; 2R01GM050231 United States GM NIGMS NIH HHS; R01 GM050231 United States GM NIGMS NIH HHS Substance Nomenclature: 0 (Drosophila Proteins) ; 0 (Transcription Factors) ; 0 (slp1 protein, Drosophila) ; 147478-66-8 (slp2 protein, Drosophila) Entry Date(s): Date Created: 20111220 Date Completed: 20120309 Latest Revision: 20211021 Update Code: 20240513 PubMed Central ID: PMC3265641

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Regulation of a duplicated locus: Drosophila sloppy paired is replete with functionally overlapping enhancers.