Effects of a pressure release on virus retention with the Ultipor DV20 membrane.
In: Biotechnology and bioengineering, Jg. 111 (2014-03-01), Heft 3, S. 545-51
Online
academicJournal
Zugriff:
Recent data have shown that a temporary release in the transmembrane pressure can cause a significant increase in virus transmission through a number of commercial virus filters. The objective of this work was to study the effect of a pressure release on retention of the bacteriophage ΦX174 by the Ultipor DV20 membrane, with phage capture within the membrane visualized by confocal microscopy. Phage challenge tests showed a significant transient increase in phage transmission (typically by an order of magnitude) immediately after a pressure release. This transient increase was not due to any damage to the filter or to the presence of a back-flow (from permeate to feed). Confocal images demonstrated that the pressure release caused the migration of previously captured bacteriophage further into the depth of the filter. Data were analyzed using a modified internal polarization model, with the results providing important insights into the factors controlling virus retention during virus filtration.
(© 2013 Wiley Periodicals, Inc.)
| Titel: |
Effects of a pressure release on virus retention with the Ultipor DV20 membrane.
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| Autor/in / Beteiligte Person: | Woods, MA ; Zydney, AL |
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| Zeitschrift: | Biotechnology and bioengineering, Jg. 111 (2014-03-01), Heft 3, S. 545-51 |
| Veröffentlichung: | <2005->: Hoboken, NJ : Wiley ; <i>Original Publication</i>: New York, Wiley., 2014 |
| Medientyp: | academicJournal |
| ISSN: | 1097-0290 (electronic) |
| DOI: | 10.1002/bit.25112 |
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