Einzeltreffer — DigiBib

Proteins encoded by figla, foxl2, scp3 and sox9a play important roles in gonad differentiation and reproduction. In the present study, we aimed to determine the responsiveness of figla, foxl2, scp3 and sox9a to 17α-ethinylestradiol (EE2) in the gonads of adult Gobiocypris rarus. Full-length cDNAs of figla, scp3 and sox9a were cloned and characterized by RT-PCR and RACE methods. Expression patterns in adult tissues were investigated. Results indicated that figla was predominantly expressed in adult ovaries and scp3 was restrictively expressed in the male testes and sox9a was principally expressed in the brains of both genders and the testes of males. Gene expression profiles of figla, foxl2, scp3 and sox9a were analyzed in the gonads of adult G. rarus exposed to EE2 at 1, 5, 25, and 125ng/L for 3 and 6days. Three-day EE2 treatment at 1-125ng/L all caused a significant increase of figla transcript in testes and foxl2 transcript in ovaries. However, six-day EE2 exposure at 1-125ng/L repressed figla and scp3 transcript in testes and foxl2 transcript in ovaries. The present study indicates that the testicular transcripts of figla and scp3 in males and the ovarian foxl2 transcript in females have high responsiveness to EE2 and they can be used as sensitive molecular biomarkers for early warning to monitor the environmental estrogenic chemicals in fresh water environment. The present study also suggests that the effective EE2 dosage for feminization in male G. rarus might be at least 25ng/L.

Titel:	Responsiveness of four gender-specific genes, figla, foxl2, scp3 and sox9a to 17α-ethinylestradiol in adult rare minnow Gobiocypris rarus.
Autor/in / Beteiligte Person:	Yuan, C ; Wu, T ; Zhang, Y ; Gao, J ; Yang, Y ; Qin, F ; Liu, S ; Zheng, Y ; Wang, Z
Zeitschrift:	General and comparative endocrinology, Jg. 200 (2014-05-01), S. 44-53
Veröffentlichung:	New York, NY : Academic Press ; <i>Original Publication</i>: New York., 2014
Medientyp:	academicJournal
ISSN:	1095-6840 (electronic)
DOI:	10.1016/j.ygcen.2014.02.018
Schlagwort:	Amino Acid Sequence Animals Cloning, Molecular Female Fish Proteins chemistry Fish Proteins metabolism Gene Expression Profiling Humans Male Molecular Sequence Data Organ Specificity genetics Phylogeny RNA, Messenger genetics RNA, Messenger metabolism Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Nucleic Acid Transcription Factors chemistry Transcription Factors metabolism Aging genetics Cyprinidae genetics Ethinyl Estradiol pharmacology Fish Proteins genetics Gene Expression Regulation drug effects Sex Characteristics Transcription Factors genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Gen Comp Endocrinol] 2014 May 01; Vol. 200, pp. 44-53. <i>Date of Electronic Publication: </i>2014 Mar 11. MeSH Terms: Sex Characteristics* ; Aging / genetics ; Cyprinidae / genetics ; Ethinyl Estradiol / pharmacology ; Fish Proteins / genetics ; Gene Expression Regulation / drug effects ; Transcription Factors / genetics ; Amino Acid Sequence ; Animals ; Cloning, Molecular ; Female ; Fish Proteins / chemistry ; Fish Proteins / metabolism ; Gene Expression Profiling ; Humans ; Male ; Molecular Sequence Data ; Organ Specificity / genetics ; Phylogeny ; RNA, Messenger / genetics ; RNA, Messenger / metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Homology, Nucleic Acid ; Transcription Factors / chemistry ; Transcription Factors / metabolism Contributed Indexing: Keywords: 17α-Ethinylestradiol; Biomarker; Gender-specific gene; Gobiocypris rarus Substance Nomenclature: 0 (Fish Proteins) ; 0 (RNA, Messenger) ; 0 (Transcription Factors) ; 423D2T571U (Ethinyl Estradiol) Entry Date(s): Date Created: 20140318 Date Completed: 20141030 Latest Revision: 20181202 Update Code: 20240513

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Responsiveness of four gender-specific genes, figla, foxl2, scp3 and sox9a to 17α-ethinylestradiol in adult rare minnow Gobiocypris rarus.