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Background: The survival promoting peptide Y-P30 has a variety of neuritogenic and neuroprotective effects in vitro and in vivo. In previous work we reported the expression of Y-P30/dermcidin in maternal peripheral blood mononuclear cells (PBMCs) and the transport of the protein to the fetal brain. In this study we analyzed hormonal regulation of Y-P30 in human immune cells and expression of Y-P30 in the placenta. We further studied the stability and secretion of the Y-P30 peptide.

Results: We found indications that Y-P30 might be produced in human placenta. The Y-P30 mRNA was rarely found in isolated human PBMCs and alpha-feto-protein, human chorionic gonadotropin as well as estradiol combined with progesterone could not induce Y-P30 expression. Y-P30 was found to be extraordinarily stable; therefore, contamination with the peptide and the Y-P30/Dermcidin precursor mRNA is a serious concern in experiments looking at the expression of Y-P30/Dermcidin. In cultured cell lines and primary neurons we found that Y-P30 could be released, but neuronal uptake of Y-P30 was not observed.

Conclusions: Our data suggest that a source of Y-P30 apart from eccrine glands might be the placenta. The peptide can be secreted together with the signaling peptide and it might reach the fetal brain where it can exert its neuritogenic functions by binding to neuronal membranes.

Titel:	Analysis of Y-P30/Dermcidin expression and properties of the Y-P30 peptide.
Autor/in / Beteiligte Person:	Mikhaylova, M ; Schumacher, A ; Borutzki, C ; Neumann, JR ; Macharadze, T ; El-Mousleh, T ; Wahle, P ; Zenclussen, AC ; Kreutz, MR
Link:	Full Text Finder (Volltext)
Zeitschrift:	BMC research notes, Jg. 7 (2014-06-26), S. 400
Veröffentlichung:	London : Biomed Central, 2008., 2014
Medientyp:	academicJournal
ISSN:	1756-0500 (electronic)
DOI:	10.1186/1756-0500-7-400
Schlagwort:	Adult Animals Blotting, Western COS Cells Cells, Cultured Chlorocebus aethiops Estradiol pharmacology Female Gene Expression drug effects Green Fluorescent Proteins genetics Green Fluorescent Proteins metabolism HEK293 Cells Humans Leukocytes, Mononuclear drug effects Microscopy, Confocal Peptides blood Peptides metabolism Pregnancy Pregnancy Trimester, First blood Progesterone pharmacology Reverse Transcriptase Polymerase Chain Reaction Syndecan-3 genetics Syndecan-3 metabolism Syndecan-4 genetics Syndecan-4 metabolism Transfection Young Adult alpha-Fetoproteins pharmacology Gene Expression genetics Leukocytes, Mononuclear metabolism Peptides genetics Placenta metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [BMC Res Notes] 2014 Jun 26; Vol. 7, pp. 400. <i>Date of Electronic Publication: </i>2014 Jun 26. MeSH Terms: Gene Expression / genetics ; Leukocytes, Mononuclear / metabolism ; Peptides / genetics ; Placenta / metabolism ; Adult ; Animals ; Blotting, Western ; COS Cells ; Cells, Cultured ; Chlorocebus aethiops ; Estradiol / pharmacology ; Female ; Gene Expression / drug effects ; Green Fluorescent Proteins / genetics ; Green Fluorescent Proteins / metabolism ; HEK293 Cells ; Humans ; Leukocytes, Mononuclear / drug effects ; Microscopy, Confocal ; Peptides / blood ; Peptides / metabolism ; Pregnancy ; Pregnancy Trimester, First / blood ; Progesterone / pharmacology ; Reverse Transcriptase Polymerase Chain Reaction ; Syndecan-3 / genetics ; Syndecan-3 / metabolism ; Syndecan-4 / genetics ; Syndecan-4 / metabolism ; Transfection ; Young Adult ; alpha-Fetoproteins / pharmacology References: FASEB J. 2005 Feb;19(2):225-7. (PMID: 15583035) ; Thrombosis. 2012;2012:987932. (PMID: 22448321) ; Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10931-6. (PMID: 12953101) ; J Biol Chem. 2006 Mar 3;281(9):5406-15. (PMID: 16354654) ; J Neurosci. 1998 Sep 15;18(18):7047-60. (PMID: 9736629) ; Eur J Appl Physiol. 2008 Dec;104(6):957-64. (PMID: 18688637) ; Clin Exp Allergy. 2011 Mar;41(3):346-56. (PMID: 21105917) ; Nature. 1996 Feb 22;379(6567):739-42. (PMID: 8602222) ; Prenat Diagn. 2012 Feb;32(2):131-7. (PMID: 22418956) ; Forensic Sci Int. 2009 Jan 30;184(1-3):47-53. (PMID: 19147311) ; Brain Struct Funct. 2015 Jul;220(4):1935-50. (PMID: 24728870) ; Eur Respir J. 2010 May;35(5):1182-5. (PMID: 20436176) ; Exp Clin Endocrinol Diabetes. 2012 Mar;120(3):145-51. (PMID: 22231923) ; J Microbiol Biotechnol. 2010 Feb;20(2):350-5. (PMID: 20208440) ; Cell. 2013 Feb 28;152(5):1119-33. (PMID: 23452857) ; Electrophoresis. 2010 Jun;31(11):1853-61. (PMID: 20506419) ; Clin Cancer Res. 2004 Sep 1;10(17):5862-9. (PMID: 15355918) ; Forensic Sci Int. 2010 Jan 30;194(1-3):80-4. (PMID: 19914015) ; Biochem Biophys Res Commun. 2007 Jun 15;357(4):828-33. (PMID: 17448443) ; Placenta. 2009 Jul;30(7):649-53. (PMID: 19481257) ; PLoS One. 2014 Feb 03;9(2):e85924. (PMID: 24498267) ; J Biol Chem. 2011 Oct 21;286(42):36762-76. (PMID: 21878631) ; Br J Cancer. 2008 Jul 8;99(1):126-32. (PMID: 18594538) ; Sci Transl Med. 2014 May 21;6(237):237ra65. (PMID: 24848255) ; Clin Cancer Res. 2007 Sep 1;13(17):4984-92. (PMID: 17785548) ; Mol Cell Neurosci. 2011 Nov;48(3):195-204. (PMID: 21820515) ; J Immunol Methods. 2002 Dec 1;270(1):53-62. (PMID: 12379338) ; Proc Natl Acad Sci U S A. 2013 Mar 19;110(12):4586-91. (PMID: 23426625) ; J Innate Immun. 2012;4(4):349-60. (PMID: 22455996) ; Biochim Biophys Acta. 2011 Jun;1812(6):703-10. (PMID: 21397687) ; Scand J Immunol. 2009 Sep;70(3):264-76. (PMID: 19703016) ; Neuroreport. 2011 Aug 3;22(11):544-7. (PMID: 21666514) ; Med Gas Res. 2012 Feb 20;2(1):4. (PMID: 22348450) ; BMC Cancer. 2008 Jan 28;8:24. (PMID: 18226207) ; Proc Natl Acad Sci U S A. 2009 Jun 2;106(22):9093-8. (PMID: 19458041) ; J Biol Chem. 2008 Sep 5;283(36):25036-45. (PMID: 18599487) ; Nat Immunol. 2001 Dec;2(12):1133-7. (PMID: 11694882) ; Biochem Soc Trans. 2009 Aug;37(Pt 4):778-82. (PMID: 19614593) ; Arch Pharm Res. 2010 Feb;33(2):247-55. (PMID: 20195826) ; Eur J Pharmacol. 2009 Aug 15;616(1-3):81-5. (PMID: 19490911) ; Am J Pathol. 2005 Mar;166(3):811-22. (PMID: 15743793) ; Am J Pathol. 2012 Feb;180(2):693-702. (PMID: 22138583) ; Exp Neurol. 2000 Jun;163(2):457-68. (PMID: 10833321) ; Comp Biochem Physiol Part D Genomics Proteomics. 2007 Dec;2(4):322-31. (PMID: 20483304) ; J Biol Chem. 2011 Apr 8;286(14):12803-11. (PMID: 21216960) Substance Nomenclature: 0 (Peptides) ; 0 (Syndecan-3) ; 0 (Syndecan-4) ; 0 (alpha-Fetoproteins) ; 0 (dermcidin) ; 147336-22-9 (Green Fluorescent Proteins) ; 4G7DS2Q64Y (Progesterone) ; 4TI98Z838E (Estradiol) Entry Date(s): Date Created: 20140628 Date Completed: 20150420 Latest Revision: 20211021 Update Code: 20240513 PubMed Central ID: PMC4082292

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Analysis of Y-P30/Dermcidin expression and properties of the Y-P30 peptide.