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Background: Shiga toxin-producing Escherichia coli (STEC) O157:H7 is a well-recognized cause of bloody diarrhea and hemolytic-uremic syndrome (HUS). The ability of STEC strains to cause human disease is due to the production of Shiga toxins. The objectives of this study were to determinate the prevalence, serotypes, antibiotic susceptibility patterns and the genetic capability for Shiga toxin production in Escherichia coli (STEC) strains isolated from dairy cattle farms in two rural communities in the Eastern Cape Province of South Africa.

Methods: Fecal samples were collected between March and May 2014, from individual cattle (n=400) in two commercial dairy farms having 800 and 120 cattle each. Three hundred presumptive isolates obtained were subjected to polymerase chain reactions (PCR) for identification of O157 serogroup and Shiga toxin producing genes (stx1, stx2) on genomic DNA extracted by boiling method. Susceptibility of the isolates to 17 antibiotics was carried out in vitro by the standardized agar disc-diffusion method.

Results: Based on direct PCR detection, 95 (31.7%) isolates were identified as O157 serogroup. The genetic repertoire for Shiga toxin production was present in 84 (88.42%) isolates distributed as stx1 (37), stx2 (38) and stx1/2 (9) respectively while 11 of the isolates did not harbor Shiga toxin producing genes. Multiple antibiotic resistances were observed among the isolates and genetic profiling of resistance genes identified bla ampC 90%, blaCMY 70%, blaCTX-M 65%, blaTEM 27% and tetA 70% and strA 80% genes among the antimicrobial resistance determinants examined.

Conclusion: We conclude that dairy cattle farms in the Eastern Cape Province are potential reservoirs of antibiotic resistance determinants in the province.

Titel:	Multiple antibiotic resistances among Shiga toxin producing Escherichia coli O157 in feces of dairy cattle farms in Eastern Cape of South Africa.
Autor/in / Beteiligte Person:	Iweriebor, BC ; Iwu, CJ ; Obi, LC ; Nwodo, UU ; Okoh, AI
Link:	Full Text Finder (Volltext)
Zeitschrift:	BMC microbiology, Jg. 15 (2015-10-16), S. 213
Veröffentlichung:	London : BioMed Central, [2001-, 2015
Medientyp:	academicJournal
ISSN:	1471-2180 (electronic)
DOI:	10.1186/s12866-015-0553-y
Schlagwort:	Agriculture Animals Cattle DNA, Bacterial genetics Disease Reservoirs Disk Diffusion Antimicrobial Tests Escherichia coli Infections epidemiology Escherichia coli Infections microbiology Escherichia coli O157 classification Escherichia coli O157 genetics Polymerase Chain Reaction Prevalence Rural Population Serogroup South Africa epidemiology Anti-Bacterial Agents pharmacology Drug Resistance, Multiple, Bacterial Escherichia coli Infections veterinary Escherichia coli O157 drug effects Escherichia coli O157 isolation & purification Feces microbiology Shiga Toxin genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [BMC Microbiol] 2015 Oct 16; Vol. 15, pp. 213. <i>Date of Electronic Publication: </i>2015 Oct 16. MeSH Terms: Drug Resistance, Multiple, Bacterial* ; Anti-Bacterial Agents / pharmacology ; Escherichia coli Infections / veterinary ; Escherichia coli O157 / drug effects ; Escherichia coli O157 / isolation & purification ; Feces / microbiology ; Shiga Toxin / genetics ; Agriculture ; Animals ; Cattle ; DNA, Bacterial / genetics ; Disease Reservoirs ; Disk Diffusion Antimicrobial Tests ; Escherichia coli Infections / epidemiology ; Escherichia coli Infections / microbiology ; Escherichia coli O157 / classification ; Escherichia coli O157 / genetics ; Polymerase Chain Reaction ; Prevalence ; Rural Population ; Serogroup ; South Africa / epidemiology References: J Food Prot. 2005 Aug;68(8):1556-65. (PMID: 21132960) ; Nat Rev Microbiol. 2004 Feb;2(2):123-40. (PMID: 15040260) ; Antimicrob Agents Chemother. 2005 Mar;49(3):1262-4. (PMID: 15728945) ; Appl Environ Microbiol. 2007 Jan;73(1):156-63. (PMID: 17098918) ; Pediatr Nephrol. 2003 Feb;18(2):92-6. (PMID: 12579394) ; Vet Microbiol. 2008 Dec 10;132(3-4):428-34. (PMID: 18603384) ; FEMS Microbiol Ecol. 2000 Apr 1;32(1):77-85. (PMID: 10779622) ; Int J Antimicrob Agents. 2006 Nov;28(5):402-7. (PMID: 17027237) ; FEMS Microbiol Lett. 1997 Jul 1;152(1):163-7. (PMID: 9228783) ; Foodborne Pathog Dis. 2010 Oct;7(10):1239-45. (PMID: 20590425) ; J Antimicrob Chemother. 2010 Apr;65(4):666-8. (PMID: 20106863) ; J Anim Sci. 2007 Mar;85(13 Suppl):E45-62. (PMID: 17085726) ; J Microbiol Biotechnol. 2010 Jun;20(6):1042-52. (PMID: 20622506) ; Diagn Microbiol Infect Dis. 2001 Sep-Oct;41(1-2):57-63. (PMID: 11687315) ; Appl Environ Microbiol. 2011 Jun;77(11):3715-9. (PMID: 21478314) ; Antimicrob Agents Chemother. 2004 Apr;48(4):1249-55. (PMID: 15047527) ; Foodborne Pathog Dis. 2010 Apr;7(4):449-57. (PMID: 19958100) ; J Clin Microbiol. 2008 Apr;46(4):1566-7. (PMID: 18272707) ; Appl Environ Microbiol. 2006 Jan;72(1):645-52. (PMID: 16391102) ; Vet Microbiol. 2007 Apr 15;121(3-4):197-214. (PMID: 17306475) ; Antimicrob Agents Chemother. 2003 Jun;47(6):2056-8. (PMID: 12760899) ; Int J Antimicrob Agents. 2007 May;29(5):576-81. (PMID: 17314033) ; Vet Microbiol. 2006 Dec 20;118(3-4):299-304. (PMID: 16973308) ; FEMS Microbiol Lett. 2002 Apr 9;209(2):161-8. (PMID: 12007800) ; Epidemiol Infect. 2001 Oct;127(2):195-206. (PMID: 11693496) ; Foodborne Pathog Dis. 2010 Dec;7(12):1575-9. (PMID: 20707724) ; Antimicrob Agents Chemother. 1999 Mar;43(3):573-81. (PMID: 10049269) ; Clin Infect Dis. 2004 Jan 1;38(1):92-8. (PMID: 14679454) ; Int Microbiol. 2004 Dec;7(4):269-76. (PMID: 15666247) ; Antimicrob Agents Chemother. 1983 Dec;24(6):835-40. (PMID: 6660856) ; Microb Drug Resist. 2006 Summer;12(2):145-8. (PMID: 16922633) ; Emerg Infect Dis. 2004 Jan;10(1):69-75. (PMID: 15078599) ; J Clin Microbiol. 2003 Mar;41(3):1109-13. (PMID: 12624037) ; FEMS Microbiol Lett. 2007 Jan;266(2):152-7. (PMID: 17233725) ; Vet Microbiol. 2010 May 19;142(3-4):445-9. (PMID: 19914011) ; Foodborne Pathog Dis. 2013 Oct;10(10):835-41. (PMID: 23909776) ; J Antimicrob Chemother. 2011 Apr;66(4):765-8. (PMID: 21393133) ; Front Microbiol. 2011 Aug 17;2:168. (PMID: 21887152) ; J Clin Microbiol. 1998 Jun;36(6):1795-7. (PMID: 9620426) ; MMWR Morb Mortal Wkly Rep. 2001 Apr 20;50(15):293-7. (PMID: 11330497) Substance Nomenclature: 0 (Anti-Bacterial Agents) ; 0 (DNA, Bacterial) ; 75757-64-1 (Shiga Toxin) Entry Date(s): Date Created: 20151018 Date Completed: 20160628 Latest Revision: 20220310 Update Code: 20231215 PubMed Central ID: PMC4609099

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Multiple antibiotic resistances among Shiga toxin producing Escherichia coli O157 in feces of dairy cattle farms in Eastern Cape of South Africa.