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Previous studies indicated that chlorogenic acid, a compound present in many fruits and vegetables, has anti-cancer activities. We report that chlorogenic acid regulates the expression of apoptosis-related genes and self-renewal-related stem cell markers in cancer cells. The lung cancer cell line A549 was cultured with or without chlorogenic acid. The presence of chlorogenic acid decreased cell proliferation as measured by MTT activity. Polymerase chain reaction (PCR) showed that treatment of cells with chlorogenic acid reduced the expression of BCL2 but increased that of both BAX and CASP3. Chlorogenic acid enhanced annexin V expression as measured using fluorescently labeled annexin V. Chlorogenic acid also induced p38 MAPK and JNK gene expression. Meanwhile, several agents, including SB203580 (p38 MAP kinase inhibitor), N-acetylcysteine (antioxidant inhibitor), dipyridamole (phosphodiesterase inhibitor), and apocynin (NADPH-oxidase inhibitor) blocked chlorogenic acid-induced BAX gene expression. Chlorogenic acid reduced gene expression levels of stem cell-associated markers NANOG, POU5F1, and SOX2. Together these results indicate that chlorogenic acid affects the expression of apoptosis-related genes that are part of oxidative stress and p38 MAP-dependent pathways, as well as genes encoding stem cell markers. In conclusion, chlorogenic acid may contribute to the polyphenolic anti-cancer effect associated with consumption of vegetables and fruits.

Titel:	Chlorogenic acid regulates apoptosis and stem cell marker-related gene expression in A549 human lung cancer cells.
Autor/in / Beteiligte Person:	Yamagata, K ; Izawa, Y ; Onodera, D ; Tagami, M
Link:	Full Text Finder (Volltext)
Zeitschrift:	Molecular and cellular biochemistry, Jg. 441 (2018-04-01), Heft 1-2, S. 9-19
Veröffentlichung:	New York : Springer ; <i>Original Publication</i>: The Hague, Dr. W. Junk B. V. Publishers., 2018
Medientyp:	academicJournal
ISSN:	1573-4919 (electronic)
DOI:	10.1007/s11010-017-3171-1
Schlagwort:	A549 Cells Biomarkers, Tumor genetics Humans Lung Neoplasms drug therapy Lung Neoplasms genetics Lung Neoplasms pathology Neoplastic Stem Cells Apoptosis drug effects Biomarkers, Tumor biosynthesis Chlorogenic Acid pharmacology Gene Expression Regulation, Neoplastic drug effects Lung Neoplasms metabolism Neoplasm Proteins biosynthesis
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Mol Cell Biochem] 2018 Apr; Vol. 441 (1-2), pp. 9-19. <i>Date of Electronic Publication: </i>2017 Sep 05. MeSH Terms: Apoptosis / drug effects ; Biomarkers, Tumor / biosynthesis ; Chlorogenic Acid / pharmacology ; Gene Expression Regulation, Neoplastic / drug effects ; Lung Neoplasms / metabolism ; Neoplasm Proteins / biosynthesis ; A549 Cells ; Biomarkers, Tumor / genetics ; Humans ; Lung Neoplasms / drug therapy ; Lung Neoplasms / genetics ; Lung Neoplasms / pathology ; Neoplastic Stem Cells References: Public Health Nutr. 2016 Oct;19(14):2603-17. (PMID: 27000627) ; Gastroenterol Clin North Am. 2002 Jun;31(2):587-623, xi. (PMID: 12134620) ; Int J Mol Med. 2015 Oct;36(4):1118-26. (PMID: 26310655) ; In Vivo. 2012 Nov-Dec;26(6):971-8. (PMID: 23160680) ; Carcinogenesis. 2007 Sep;28(9):1946-53. (PMID: 17675339) ; Ann Ist Super Sanita. 2007;43(4):348-61. (PMID: 18209268) ; Neuroscience. 2014 May 16;267:277-85. (PMID: 24613720) ; PLoS One. 2008;3(10):e3360. (PMID: 18852874) ; World J Clin Oncol. 2014 Aug 10;5(3):520-8. (PMID: 25114865) ; Dev Biol. 2008 May 1;317(1):296-309. (PMID: 18374910) ; Pharm World Sci. 1997 Jun;19(3):119-25. (PMID: 9259027) ; Life Sci. 2016 Oct 1;162:77-86. (PMID: 27569589) ; Anticancer Drugs. 2017 Jan;28(1):59-65. (PMID: 27603595) ; Nature. 2013 Sep 19;501(7467):328-37. (PMID: 24048065) ; Mol Cancer. 2017 Mar 14;16(1):62. (PMID: 28288641) ; J Clin Oncol. 2004 Jan 15;22(2):330-53. (PMID: 14691125) ; J Biol Chem. 2006 May 19;281(20):14026-40. (PMID: 16565089) ; Clin Cancer Res. 2010 May 1;16(9):2580-90. (PMID: 20388854) ; Stem Cells Dev. 2009 Sep;18(7):1093-108. (PMID: 19480567) ; Mol Med Rep. 2015 Aug;12(2):2977-84. (PMID: 25936427) ; Microvasc Res. 2013 Jul;88:25-31. (PMID: 23567873) ; Cancer Lett. 2016 Mar 28;372(2):147-56. (PMID: 26797015) ; Int J Oncol. 2014 May;44(5):1652-60. (PMID: 24573690) ; Leuk Lymphoma. 1998 Jan;28(3-4):355-61. (PMID: 9517506) ; Curr Cancer Drug Targets. 2005 Mar;5(2):117-29. (PMID: 15810876) ; Carcinogenesis. 2007 Jun;28(6):1277-86. (PMID: 17277231) ; Cancer Res. 2004 Jan 15;64(2):696-703. (PMID: 14744787) ; J Cell Biochem. 1996 Jan;60(1):23-32. (PMID: 8825412) ; Genome Res. 1996 Oct;6(10):995-1001. (PMID: 8908519) ; Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7834-8. (PMID: 7644501) ; Phytochemistry. 2012 May;77:10-5. (PMID: 22377690) ; Haematologica. 2016 Jan;101(1):77-85. (PMID: 26565002) ; Curr Top Microbiol Immunol. 1995;200:107-21. (PMID: 7634826) ; Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):973-8. (PMID: 17210912) ; Stem Cells. 2010 May;28(5):885-93. (PMID: 20333750) ; Apoptosis. 2015 Aug;20(8):1109-21. (PMID: 26002438) ; Oncotarget. 2015 May 10;6(13):10697-711. (PMID: 25986923) ; Food Funct. 2012 Sep;3(9):916-22. (PMID: 22627289) ; Food Chem Toxicol. 2000 May;38(5):467-71. (PMID: 10762733) ; Asian Pac J Cancer Prev. 2014;15(11):4519-25. (PMID: 24969879) ; Cancer Res. 1988 Nov 1;48(21):5941-6. (PMID: 3139287) ; Mol Nutr Food Res. 2008 May;52(5):507-26. (PMID: 18435439) ; Food Funct. 2015 Mar;6(3):995-1000. (PMID: 25675448) ; Science. 1999 Feb 19;283(5405):1183-6. (PMID: 10024247) ; Korean J Physiol Pharmacol. 2011 Feb;15(1):1-7. (PMID: 21461234) ; J Biol Chem. 2009 Jan 30;284(5):2657-71. (PMID: 19047049) ; J Natl Cancer Inst. 2017 Mar 1;109 (3):1-3. (PMID: 28376203) ; Cell. 2003 May 30;113(5):631-42. (PMID: 12787504) ; Mol Med Rep. 2013 Oct;8(4):1106-10. (PMID: 23982123) ; World J Clin Oncol. 2014 Oct 10;5(4):588-94. (PMID: 25302163) ; Int J Cancer. 2016 Dec 15;139(12 ):2714-2721. (PMID: 27560973) ; Int J Exp Pathol. 2016 Apr;97(2):97-106. (PMID: 27126918) ; Onco Targets Ther. 2017 Mar 14;10 :1561-1573. (PMID: 28352189) Contributed Indexing: Keywords: Apoptosis; Chlorogenic acid; Stem cell markers Substance Nomenclature: 0 (Biomarkers, Tumor) ; 0 (Neoplasm Proteins) ; 318ADP12RI (Chlorogenic Acid) Entry Date(s): Date Created: 20170907 Date Completed: 20180710 Latest Revision: 20181113 Update Code: 20231215

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Chlorogenic acid regulates apoptosis and stem cell marker-related gene expression in A549 human lung cancer cells.