Glucocorticoid receptor is involved in the differential expression of hepatic 3β-hydroxysteroid dehydrogenase between barrows and boars at finishing stage.
In: Animal science journal = Nihon chikusan Gakkaiho, Jg. 89 (2018), Heft 1, S. 158-166
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Zugriff:
The enzyme 3β-hydroxysteroid dehydrogenase (3β-HSD) plays an important role in androstenone metabolism in pig liver, and its defective expression is related to the development of boar taint. Early age castration is a common practice in many countries to avoid boar taint, yet whether and how castration affects porcine hepatic 3β-HSD expression are still poorly understood. In this study, we aimed to compare the expression of 3β-HSD between intact (boars) and castrated (barrows) male pigs, and to explore the potential factors regulating 3β-HSD transcription. Compared to barrows, boars showed worse carcass quality. Boars had significantly higher levels of serum androstenone (P < 0.01), testosterone (P < 0.01) and hepatic cortisol (P < 0.05), which were contrary to significantly lower expression of 3β-HSD messenger RNA (P < 0.01) and protein (P < 0.01) in the liver. Significant differences were detected for the hepatic expression of androgen receptor (AR) and CCAAT/enhancer binding protein β (C/EBPβ). Chromatin immunoprecipitation (ChIP) assay demonstrated reduced histone H3 acetylation (P < 0.05) but increased glucocorticoid receptor (GR) binding to 3β-HSD gene promoter in boars (P < 0.05). These results indicate that GR binding to 3β-HSD promoter is involved in the differential hepatic 3β-HSD expression between boars and barrows.
(© 2017 Japanese Society of Animal Science.)
Titel: |
Glucocorticoid receptor is involved in the differential expression of hepatic 3β-hydroxysteroid dehydrogenase between barrows and boars at finishing stage.
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Autor/in / Beteiligte Person: | Li, X ; Cong, R ; Yao, W ; Jia, Y ; Li, R ; Sun, Z ; Zhao, R |
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Zeitschrift: | Animal science journal = Nihon chikusan Gakkaiho, Jg. 89 (2018), Heft 1, S. 158-166 |
Veröffentlichung: | Richmond, Vic. : Wiley ; <i>Original Publication</i>: Tokyo, Japan : Japanese Society of Zootechnical Science [1999-, 2018 |
Medientyp: | academicJournal |
ISSN: | 1740-0929 (electronic) |
DOI: | 10.1111/asj.12853 |
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