Correlation between S100A11 and the TGF-β <subscript>1</subscript> /SMAD4 pathway and its effects on the proliferation and apoptosis of pancreatic cancer cell line PANC-1.
In: Molecular and cellular biochemistry, Jg. 450 (2019), Heft 1-2, S. 53-64
Online
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Zugriff:
S100A11 as a S100 protein family member has been documented to play dual-direction regulation over cancer cell proliferation. We explored the role of S100A11 in the proliferation and apoptosis of pancreatic cancer cell line PANC-1 and the potential mechanisms involving the TGF-β 1 /SMAD4/p21 pathway. S100A11 and TGF-β 1 protein expressions in 30 paraffin-embedded specimens were evaluated by immunohistochemistry. S100A11 and TGF-β 1 expression in PANC-1 cell line was suppressed using small interfering RNA (siRNA), respectively. Subsequently, pancreatic cancer cell apoptosis was measured by Cell Counting Kit-8 and flow cytometry, and S100A11 and TGF-β1/SMAD4/p21 pathway proteins and genes were detected with Western blotting and quantitative polymerase chain reaction (qPCR). S100A11 cytoplasmic/nuclear protein translocation was examined using NE-PER® cytoplasm/nuclear protein extraction in cells interfered with TGF-β1 siRNA. Our results showed that S100A11 expression was positively correlated with TGF-β 1 expression in pancreatic cancerous tissue. Silencing TGF-β 1 down-regulated intracellular P21 WAF1 expression by 90%, blocked S100A11 from cytoplasm entering nucleus, and enhanced cell proliferation. Silencing S100A11 down-regulated intracellular P21 expression and promoted cell apoptosis without significantly changing TGF-β 1 and SMAD4 expression. Our findings revealed that S100A11 and TGF-β 1 /SMAD4 signaling pathway were related but mutually independent in regulating PANC-1 cells proliferation and apoptosis. Other independent mechanisms might be involved in S100A11's regulation of pancreatic cell growth. S100A11 could be a potential gene therapy target for pancreatic cancer.
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Correlation between S100A11 and the TGF-β <subscript>1</subscript> /SMAD4 pathway and its effects on the proliferation and apoptosis of pancreatic cancer cell line PANC-1.
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Autor/in / Beteiligte Person: | Ji, YF ; Li, T ; Jiang, F ; Ni, WK ; Guan, CQ ; Liu, ZX ; Lu, CH ; Ni, RZ ; Wu, W ; Xiao, MB |
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Zeitschrift: | Molecular and cellular biochemistry, Jg. 450 (2019), Heft 1-2, S. 53-64 |
Veröffentlichung: | New York : Springer ; <i>Original Publication</i>: The Hague, Dr. W. Junk B. V. Publishers., 2019 |
Medientyp: | academicJournal |
ISSN: | 1573-4919 (electronic) |
DOI: | 10.1007/s11010-018-3372-2 |
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