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Male infertility is a rising problem around the world. Often the cause of male infertility is unclear, and this hampers diagnosis and treatment. Spermatogenesis is a complex process under sophisticated regulation by many testis-specific genes. Here, we report the testis-specific gene 1700102P08Rik is conserved in both the human and mouse and highly expressed in spermatocytes. To investigate the role of 1700102P08Rik in male fertility, knockout mice were generated by CRISPR-Cas9. 1700102P08Rik knockout male mice were infertile with smaller testis and epididymis, but female knockout mice retained normal fertility. Spermatogenesis in the 1700102P08Rik knockout male mouse was arrested at the spermatocyte stage, and no sperm were found in the epididymis. The deletion of 1700102P08Rik causes apoptosis in the testis but did not affect the serum concentration of testosterone, luteinizing hormone, and follicle-stimulating hormone or the synapsis and recombination of homologous chromosomes. We also found that 1700102P08Rik is downregulated in spermatocyte arrest in men. Together, these results indicate that the 1700102P08Rik gene is essential for spermatogenesis and its dysfunction leads to male infertility.

Titel:	The testis-specific gene 1700102P08Rik is essential for male fertility.
Autor/in / Beteiligte Person:	Wu, XL ; Yun, DM ; Gao, S ; Liang, AJ ; Duan, ZZ ; Wang, HS ; Wang, GS ; Sun, F
Link:	Full Text Finder (Volltext)
Zeitschrift:	Molecular reproduction and development, Jg. 87 (2020-02-01), Heft 2, S. 231-240
Veröffentlichung:	<2005-> : Hoboken, N.J. : Wiley-Liss ; <i>Original Publication</i>: New York, NY : A.R. Liss, 1988-, 2020
Medientyp:	academicJournal
ISSN:	1098-2795 (electronic)
DOI:	10.1002/mrd.23314
Schlagwort:	Animals Apoptosis genetics Cells, Cultured Down-Regulation genetics Female Follicle Stimulating Hormone, Human blood Gene Knockout Techniques Humans Infertility, Male blood Luteinizing Hormone blood Male Mice Mice, Inbred C57BL Mice, Inbred ICR Mice, Knockout Spermatocytes metabolism Spermatogenesis genetics Testis pathology Testosterone blood Fertility genetics Genes, Essential Infertility, Male genetics Intercellular Signaling Peptides and Proteins genetics Proteins genetics Testis physiopathology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Mol Reprod Dev] 2020 Feb; Vol. 87 (2), pp. 231-240. <i>Date of Electronic Publication: </i>2020 Jan 12. MeSH Terms: Genes, Essential* ; Fertility / genetics ; Infertility, Male / genetics ; Intercellular Signaling Peptides and Proteins / genetics ; Proteins / genetics ; Testis / *physiopathology ; Animals ; Apoptosis / genetics ; Cells, Cultured ; Down-Regulation / genetics ; Female ; Follicle Stimulating Hormone, Human / blood ; Gene Knockout Techniques ; Humans ; Infertility, Male / blood ; Luteinizing Hormone / blood ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Inbred ICR ; Mice, Knockout ; Spermatocytes / metabolism ; Spermatogenesis / genetics ; Testis / pathology ; Testosterone / blood References: Anawalt, B. D. (2013). Approach to male infertility and induction of spermatogenesis. 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Journal of Cell Science, 124(Pt 4), 501-513. https://doi.org/10.1242/jcs.074427. Contributed Indexing: Keywords: 1700102P08Rik; male infertility; spermatogenesis Substance Nomenclature: 0 (1700102P08Rik protein, mouse) ; 0 (C3orf62 protein, human) ; 0 (Follicle Stimulating Hormone, Human) ; 0 (Intercellular Signaling Peptides and Proteins) ; 0 (Proteins) ; 3XMK78S47O (Testosterone) ; 9002-67-9 (Luteinizing Hormone) Entry Date(s): Date Created: 20200114 Date Completed: 20210111 Latest Revision: 20210202 Update Code: 20231215

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The testis-specific gene 1700102P08Rik is essential for male fertility.