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Enteric pathogens exploit chemical and nutrient signaling to gauge their location within a host and control expression of traits important for infection. Ethanolamine-containing molecules are essential in host physiology and play important roles in intestinal processes. The transcription factor EutR is conserved in the Enterobacteriaceae and is required for ethanolamine sensing and metabolism. In enterohemorrhagic Escherichia coli (EHEC) O157:H7, EutR responds to ethanolamine to activate expression of traits required for host colonization and disease; however, the importance of EutR to EHEC intestinal infection has not been examined. Because EHEC does not naturally colonize or cause disease in mice, we employed the natural murine pathogen Citrobacter rodentium as a model of EHEC virulence to investigate the importance of EutR in vivo EHEC and C. rodentium possess the locus of enterocyte effacement (LEE), which is the canonical virulence trait of attaching and effacing pathogens. Our findings demonstrate that ethanolamine sensing and EutR-dependent regulation of the LEE are conserved in C. rodentium Moreover, during infection, EutR is required for maximal LEE expression, colonization, and transmission efficiency. These findings reveal that EutR not only is important for persistence during the primary host infection cycle but also is required for maintenance in a host population.

Titel:	The Ethanolamine-Sensing Transcription Factor EutR Promotes Virulence and Transmission during Citrobacter rodentium Intestinal Infection.
Autor/in / Beteiligte Person:	Rowley, CA ; Sauder, AB ; Kendall, MM
Link:	Full Text Finder (Volltext)
Zeitschrift:	Infection and immunity, Jg. 88 (2020-08-19), Heft 9
Veröffentlichung:	Washington, DC : American Society For Microbiology ; <i>Original Publication</i>: [Bethesda, Md.] American Society for Microbiology., 2020
Medientyp:	academicJournal
ISSN:	1098-5522 (electronic)
DOI:	10.1128/IAI.00137-20
Schlagwort:	Animals Citrobacter rodentium pathogenicity Colony Count, Microbial Conserved Sequence Enterobacteriaceae Infections genetics Enterobacteriaceae Infections pathology Enterobacteriaceae Infections transmission Enterocytes microbiology Enterocytes pathology Enterohemorrhagic Escherichia coli pathogenicity Escherichia coli Proteins metabolism Female Host Microbial Interactions genetics Mice Mice, Inbred C57BL Mice, Knockout Phosphoproteins metabolism Protein Isoforms deficiency Protein Isoforms genetics Signal Transduction Transcription Factors deficiency Virulence Citrobacter rodentium genetics Enterobacteriaceae Infections microbiology Enterohemorrhagic Escherichia coli genetics Escherichia coli Proteins genetics Ethanolamine metabolism Gene Expression Regulation, Bacterial Phosphoproteins genetics Transcription Factors genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Language: English [Infect Immun] 2020 Aug 19; Vol. 88 (9). <i>Date of Electronic Publication: </i>2020 Aug 19 (<i>Print Publication: </i>2020). MeSH Terms: Gene Expression Regulation, Bacterial* ; Citrobacter rodentium / genetics ; Enterobacteriaceae Infections / microbiology ; Enterohemorrhagic Escherichia coli / genetics ; Escherichia coli Proteins / genetics ; Ethanolamine / metabolism ; Phosphoproteins / genetics ; Transcription Factors / *genetics ; Animals ; Citrobacter rodentium / pathogenicity ; Colony Count, Microbial ; Conserved Sequence ; Enterobacteriaceae Infections / genetics ; Enterobacteriaceae Infections / pathology ; Enterobacteriaceae Infections / transmission ; Enterocytes / microbiology ; Enterocytes / pathology ; Enterohemorrhagic Escherichia coli / pathogenicity ; Escherichia coli Proteins / metabolism ; Female ; Host Microbial Interactions / genetics ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Phosphoproteins / metabolism ; Protein Isoforms / deficiency ; Protein Isoforms / genetics ; Signal Transduction ; Transcription Factors / deficiency ; Virulence References: J Bacteriol. 1988 Sep;170(9):3855-63. (PMID: 3045078) ; Cell. 1997 Nov 14;91(4):511-20. (PMID: 9390560) ; Proc Natl Acad Sci U S A. 2019 Jul 9;116(28):14210-14215. (PMID: 31235565) ; Science. 2016 Sep 16;353(6305):1249-53. (PMID: 27634526) ; Infect Immun. 2014 Jan;82(1):193-201. (PMID: 24126525) ; J Bacteriol. 1999 Sep;181(17):5317-29. (PMID: 10464203) ; Science. 2014 Aug 22;345(6199):940-3. (PMID: 25146292) ; J Bacteriol. 2004 Nov;186(22):7635-44. (PMID: 15516577) ; Nat Rev Microbiol. 2010 Apr;8(4):290-5. (PMID: 20234377) ; Microbes Infect. 2005 Jun;7(7-8):997-1004. (PMID: 15925533) ; Environ Microbiol. 2011 Feb;13(2):365-77. (PMID: 20849446) ; Lancet. 1983 Dec 3;2(8362):1299-1300. (PMID: 6139632) ; mBio. 2018 Oct 2;9(5):. (PMID: 30279284) ; Cell Microbiol. 2000 Dec;2(6):579-90. (PMID: 11207610) ; Virulence. 2013 May 15;4(4):315-23. (PMID: 23552827) ; Mol Microbiol. 1999 Sep;33(6):1176-89. (PMID: 10510232) ; Nat Rev Microbiol. 2014 Sep;12(9):612-23. (PMID: 25088150) ; Stand Genomic Sci. 2017 Oct 10;12:61. (PMID: 29046740) ; Microb Pathog. 2018 Jun;119:200-207. (PMID: 29654901) ; Mol Microbiol. 1999 Jul;33(2):296-306. (PMID: 10411746) ; Infect Immun. 2001 Jun;69(6):4027-33. (PMID: 11349072) ; Infect Immun. 2005 Jul;73(7):4327-37. (PMID: 15972527) ; J Bacteriol. 2001 May;183(9):2823-33. (PMID: 11292802) ; J Bacteriol. 1992 Oct;174(20):6634-43. (PMID: 1328159) ; Cell Microbiol. 2005 Aug;7(8):1163-72. (PMID: 16008583) ; Nat Rev Microbiol. 2004 Feb;2(2):123-40. (PMID: 15040260) ; Mol Microbiol. 1996 Apr;20(2):313-23. (PMID: 8733230) ; J Bacteriol. 2013 Nov;195(21):4947-53. (PMID: 23995630) ; Infect Immun. 2007 May;75(5):2634-7. (PMID: 17307944) ; Infect Immun. 2003 Jan;71(1):384-92. (PMID: 12496188) ; Nature. 2001 Jan 25;409(6819):529-33. (PMID: 11206551) ; Infect Immun. 2018 Apr 23;86(5):. (PMID: 29531136) ; J Bacteriol. 2009 Dec;191(23):7157-64. (PMID: 19783625) ; J Bacteriol. 2010 Jan;192(2):525-38. (PMID: 19897651) ; PLoS One. 2016 Jan 28;11(1):e0148023. (PMID: 26821228) ; Nat Rev Microbiol. 2019 Nov;17(11):701-715. (PMID: 31541196) ; Cell Microbiol. 2005 Dec;7(12):1697-706. (PMID: 16309456) ; Infect Immun. 1993 Jun;61(6):2486-92. (PMID: 8500884) ; Infect Immun. 2001 Oct;69(10):6323-35. (PMID: 11553577) ; Environ Microbiol. 2018 Apr;20(4):1419-1435. (PMID: 29349925) ; PLoS Pathog. 2017 Aug 14;13(8):e1006545. (PMID: 28806780) ; Proc Natl Acad Sci U S A. 1990 Oct;87(20):7839-43. (PMID: 2172966) ; PLoS Pathog. 2015 Nov 13;11(11):e1005278. (PMID: 26565973) ; Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5. (PMID: 10829079) ; Mol Microbiol. 1998 Apr;28(1):1-4. (PMID: 9593291) ; Curr Opin Microbiol. 2016 Feb;29:68-73. (PMID: 26707739) ; EBioMedicine. 2019 May;43:325-332. (PMID: 31036531) ; Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7996-8000. (PMID: 7644527) ; Proc Natl Acad Sci U S A. 2007 Oct 16;104(42):16669-74. (PMID: 17925449) ; Mol Microbiol. 2000 Nov;38(4):781-93. (PMID: 11115113) ; Nucleic Acids Res. 2019 Jul 2;47(W1):W636-W641. (PMID: 30976793) ; J Bacteriol. 1989 Jun;171(6):3316-23. (PMID: 2656649) ; Mol Biotechnol. 2004 Feb;26(2):117-22. (PMID: 14764937) ; Infect Immun. 2015 Apr;83(4):1384-95. (PMID: 25624355) ; FEMS Microbiol Lett. 1998 Sep 1;166(1):71-8. (PMID: 9741085) ; Proc Natl Acad Sci U S A. 2011 Oct 18;108(42):17480-5. (PMID: 21969563) ; Infect Immun. 2018 Feb 20;86(3):. (PMID: 29229730) ; Gut. 1972 Sep;13(9):675-81. (PMID: 4639402) ; Lab Anim Sci. 1976 Dec;26(6 Pt 1):889-94. (PMID: 1018473) ; J Bacteriol. 1995 Mar;177(5):1357-66. (PMID: 7868611) ; mBio. 2012 May 15;3(3):. (PMID: 22589288) ; Methods. 2009 Jan;47(1):63-72. (PMID: 18955146) Grant Information: R01 AI118732 United States AI NIAID NIH HHS; R21 AI130439 United States AI NIAID NIH HHS; T32 AI007046 United States AI NIAID NIH HHS; R03 AI146888 United States AI NIAID NIH HHS; T32 AI055432 United States AI NIAID NIH HHS Contributed Indexing: Keywords: Citrobacter; EHEC; enteric; ethanolamine; virulence Substance Nomenclature: 0 (Escherichia coli Proteins) ; 0 (EutR protein, E coli) ; 0 (LEE protein, E coli) ; 0 (Phosphoproteins) ; 0 (Protein Isoforms) ; 0 (Transcription Factors) ; 5KV86114PT (Ethanolamine) Entry Date(s): Date Created: 20200708 Date Completed: 20210303 Latest Revision: 20210303 Update Code: 20240513 PubMed Central ID: PMC7440760

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The Ethanolamine-Sensing Transcription Factor EutR Promotes Virulence and Transmission during Citrobacter rodentium Intestinal Infection.