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Estrogen has known anti-inflammatory effects, but the mechanism whereby 17β-estradiol (E2) protects oviduct sheep epithelial cells from inflammation remains unknown. In this study, we detected the E2 synthetase and E2 nuclear and membrane receptors in sheep oviducts, primarily in epithelial cells. Using lipopolysaccharide (LPS)-stimulated sheep oviduct epithelial cells as an in vitro inflammation model, we demonstrated that E2 attenuates the expression of inflammatory factors in a concentration-response manner. E2 also inhibited the LPS-stimulated phosphorylation of p38 MAPK and NF-κB p65 but did not reduce the phosphorylation of JNK and ERK 1/2. This attenuation was partially antagonized by an intracellular estrogen antagonist that was involved in genomic regulation and enhanced by a G protein-coupled estrogen receptor agonist that was involved in nongenomic cellular modulation. These results suggest that E2 has an inhibitory effect on LPS-induced oviduct epithelial cell inflammation in sheep, which is mediated by the downstream regulatory effects of estrogen receptors.

Titel:	17β-estradiol protects sheep oviduct epithelial cells against lipopolysaccharide-induced inflammation in vitro.
Autor/in / Beteiligte Person:	Ge, W ; Duan, H ; Xiao, L ; Lv, J ; Jiang, Y ; Ding, Z ; Hu, J ; Zhang, Y ; Zhao, X
Zeitschrift:	Molecular immunology, Jg. 127 (2020-11-01), S. 21-30
Veröffentlichung:	Oxford, Elmsford, N. Y., Pergamon Press., 2020
Medientyp:	academicJournal
ISSN:	1872-9142 (electronic)
DOI:	10.1016/j.molimm.2020.08.016
Schlagwort:	Animals Aromatase metabolism Cytokines genetics Cytokines metabolism Epithelial Cells drug effects Epithelial Cells metabolism Female Fulvestrant pharmacology Gene Expression Regulation drug effects Imidazoles pharmacology Inflammation genetics Lipopolysaccharides Mitogen-Activated Protein Kinases metabolism NF-kappa B metabolism Phosphatidylinositol 3-Kinases metabolism Phosphorylation Protein Kinase Inhibitors pharmacology Proto-Oncogene Proteins c-akt metabolism Pyridines pharmacology RNA, Messenger genetics RNA, Messenger metabolism Receptors, Estrogen metabolism Receptors, G-Protein-Coupled metabolism Sheep Signal Transduction drug effects Epithelial Cells pathology Estradiol pharmacology Inflammation pathology Oviducts pathology Protective Agents pharmacology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Mol Immunol] 2020 Nov; Vol. 127, pp. 21-30. <i>Date of Electronic Publication: </i>2020 Sep 06. MeSH Terms: Epithelial Cells / pathology ; Estradiol / pharmacology ; Inflammation / pathology ; Oviducts / pathology ; Protective Agents / *pharmacology ; Animals ; Aromatase / metabolism ; Cytokines / genetics ; Cytokines / metabolism ; Epithelial Cells / drug effects ; Epithelial Cells / metabolism ; Female ; Fulvestrant / pharmacology ; Gene Expression Regulation / drug effects ; Imidazoles / pharmacology ; Inflammation / genetics ; Lipopolysaccharides ; Mitogen-Activated Protein Kinases / metabolism ; NF-kappa B / metabolism ; Phosphatidylinositol 3-Kinases / metabolism ; Phosphorylation ; Protein Kinase Inhibitors / pharmacology ; Proto-Oncogene Proteins c-akt / metabolism ; Pyridines / pharmacology ; RNA, Messenger / genetics ; RNA, Messenger / metabolism ; Receptors, Estrogen / metabolism ; Receptors, G-Protein-Coupled / metabolism ; Sheep ; Signal Transduction / drug effects Contributed Indexing: Keywords: 17β-estradiol; estrogen receptor; inﬂammation; oviduct epithelial cell; sheep Substance Nomenclature: 0 (Cytokines) ; 0 (Imidazoles) ; 0 (Lipopolysaccharides) ; 0 (NF-kappa B) ; 0 (Protective Agents) ; 0 (Protein Kinase Inhibitors) ; 0 (Pyridines) ; 0 (RNA, Messenger) ; 0 (Receptors, Estrogen) ; 0 (Receptors, G-Protein-Coupled) ; 22X328QOC4 (Fulvestrant) ; 4TI98Z838E (Estradiol) ; EC 1.14.14.1 (Aromatase) ; EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) ; EC 2.7.11.24 (Mitogen-Activated Protein Kinases) ; OU13V1EYWQ (SB 203580) Entry Date(s): Date Created: 20200909 Date Completed: 20201130 Latest Revision: 20201130 Update Code: 20231215

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17β-estradiol protects sheep oviduct epithelial cells against lipopolysaccharide-induced inflammation in vitro.