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Human endometrial stem cells (hEnSCs) that can be differentiated into various neural cell types have been regarded as a suitable cell population for neural tissue engineering and regenerative medicine. Considering different interactions between hormones, growth factors, and other factors in the neural system, several differentiation protocols have been proposed to direct hEnSCs towards specific neural cells. The 17β-estradiol plays important roles in the processes of development, maturation, and function of nervous system. In the present research, the impact of 17β-estradiol (estrogen, E2) on the neural differentiation of hEnSCs was examined for the first time, based on the expression levels of neural genes and proteins. In this regard, hEnSCs were differentiated into neuron-like cells after exposure to retinoic acid (RA), epidermal growth factor (EGF), and also fibroblast growth factor-2 (FGF2) in the absence or presence of 17β-estradiol. The majority of cells showed a multipolar morphology. In all groups, the expression levels of nestin, Tuj-1 and NF-H (neurofilament heavy polypeptide) (as neural-specific markers) increased during 14 days. According to the outcomes of immunofluorescence (IF) and real-time PCR analyses, the neuron-specific markers were more expressed in the estrogen-treated groups, in comparison with the estrogen-free ones. These findings suggest that 17β-estradiol along with other growth factors can stimulate and upregulate the expression of neural markers during the neuronal differentiation of hEnSCs. Moreover, our findings confirm that hEnSCs can be an appropriate cell source for cell therapy of neurodegenerative diseases and neural tissue engineering.

Titel:	Defining the role of 17β-estradiol in human endometrial stem cells differentiation into neuron-like cells.
Autor/in / Beteiligte Person:	Hasanzadeh, E ; Ebrahimi-Barough, S ; Mahmoodi, N ; Mellati, A ; Nekounam, H ; Basiri, A ; Asadpour, S ; Ghasemi, D ; Ai, J
Link:	Full Text Finder (Volltext)
Zeitschrift:	Cell biology international, Jg. 45 (2021), Heft 1, S. 140-153
Veröffentlichung:	2013- : John Wiley & Sons Chichester : ; <i>Original Publication</i>: London, UK : Published for the International Federation for Cell Biology by Academic Press, c1993-, 2021
Medientyp:	academicJournal
ISSN:	1095-8355 (electronic)
DOI:	10.1002/cbin.11478
Schlagwort:	Biomarkers metabolism Cell Lineage Cell Shape Cells, Cultured Female Humans Cell Differentiation Endometrium cytology Estradiol pharmacology Neurons cytology Stem Cells cytology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Cell Biol Int] 2021 Jan; Vol. 45 (1), pp. 140-153. <i>Date of Electronic Publication: </i>2020 Dec 01. MeSH Terms: Cell Differentiation* ; Endometrium / cytology ; Estradiol / pharmacology ; Neurons / cytology ; Stem Cells / cytology ; Biomarkers / metabolism ; Cell Lineage ; Cell Shape ; Cells, Cultured ; Female ; Humans References: Adegani, F. J., Langroudi, L., Arefian, E., Shafiee, A., Dinarvand, P., & Soleimani, M. (2013). A comparison of pluripotency and differentiation status of four mesenchymal adult stem cells. Molecular Biology Reports, 40, 3693-3703. ; Arevalo, M. A., Santos-Galindo, M., Bellini, M. J., Azcoitia, I., & Garcia-Segura, L. M. (2010). Actions of estrogens on glial cells: Implications for neuroprotection. Biochimica et Biophysica Acta/General Subjects, 1800, 1106-1112. ; Bianchi, F., Malboubi, M., Li, Y., George, J. 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Grant Information: 34276-87-01-96s Tehran University of Medical Sciences and Health Services Contributed Indexing: Keywords: estrogen; human endometrial stem cells; neural differentiation; neural tissue engineering; neurons Substance Nomenclature: 0 (Biomarkers) ; 4TI98Z838E (Estradiol) Entry Date(s): Date Created: 20201013 Date Completed: 20210811 Latest Revision: 20210811 Update Code: 20231215

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Defining the role of 17β-estradiol in human endometrial stem cells differentiation into neuron-like cells.