Einzeltreffer — DigiBib

Typical enteropathogenic Escherichia coli (tEPEC) is a leading cause of diarrhea and associated death in children worldwide. Atypical EPEC (aEPEC) lacks the plasmid encoding bundle-forming pili and is considered less virulent, but the molecular mechanism of virulence is poorly understood. We recently identified kittens as a host for aEPEC where intestinal epithelial colonization was associated with diarrheal disease and death. The purposes of this study were to (i) determine the genomic similarity between kitten aEPEC and human aEPEC isolates and (ii) identify genotypic or phenotypic traits associated with virulence in kitten aEPEC. We observed no differences between kitten and human aEPEC in core genome content or gene cluster sequence identities, and no distinguishing genomic content was observed between aEPEC isolates from kittens with nonclinical colonization (NC) versus those with lethal infection (LI). Variation in adherence patterns and ability to aggregate actin in cultured cells mirrored descriptions of human aEPEC. The aEPEC isolated from kittens with LI were significantly more motile than isolates from kittens with NC. Kittens may serve as a reservoir for aEPEC that is indistinguishable from human aEPEC isolates and may provide a needed comparative animal model for the study of aEPEC pathogenesis. Motility seems to be an important factor in pathogenesis of LI associated with aEPEC in kittens.

Titel:	Comparative Genomics of Atypical Enteropathogenic Escherichia coli from Kittens and Children Identifies Bacterial Factors Associated with Virulence in Kittens.
Autor/in / Beteiligte Person:	Watson, VE ; Hazen, TH ; Rasko, DA ; Jacob, ME ; Elfenbein, JR ; Stauffer, SH ; Gookin, JL
Link:	Full Text Finder (Volltext)
Zeitschrift:	Infection and immunity, Jg. 89 (2021-02-16), Heft 3
Veröffentlichung:	Washington, DC : American Society For Microbiology ; <i>Original Publication</i>: [Bethesda, Md.] American Society for Microbiology., 2021
Medientyp:	academicJournal
ISSN:	1098-5522 (electronic)
DOI:	10.1128/IAI.00619-20
Schlagwort:	Adolescent Animals Child Child, Preschool Escherichia coli Infections microbiology Female Genetic Variation Humans Infant Infant, Newborn Male Serogroup Cats genetics Enteropathogenic Escherichia coli genetics Escherichia coli Infections genetics Escherichia coli Proteins genetics Genomics Serotyping Virulence genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Language: English [Infect Immun] 2021 Feb 16; Vol. 89 (3). <i>Date of Electronic Publication: </i>2021 Feb 16 (<i>Print Publication: </i>2021). MeSH Terms: Genomics* ; Serotyping* ; Cats / genetics ; Enteropathogenic Escherichia coli / genetics ; Escherichia coli Infections / genetics ; Escherichia coli Proteins / genetics ; Virulence / *genetics ; Adolescent ; Animals ; Child ; Child, Preschool ; Escherichia coli Infections / microbiology ; Female ; Genetic Variation ; Humans ; Infant ; Infant, Newborn ; Male ; Serogroup Comments: Comment in: Infect Immun. 2021 Feb 16;89(3):. (PMID: 33361199) References: J Med Microbiol. 2019 Jun;68(6):940-951. (PMID: 31107199) ; J Med Microbiol. 2004 Nov;53(Pt 11):1137-1144. (PMID: 15496393) ; BMC Bioinformatics. 2005 Jan 05;6:2. (PMID: 15634352) ; Biochimie. 2013 Feb;95(2):410-8. (PMID: 23123524) ; J Clin Microbiol. 2015 Aug;53(8):2410-26. (PMID: 25972421) ; Nat Microbiol. 2016 Jan 18;1:15014. (PMID: 27571975) ; Zoonoses Public Health. 2009 Jun;56(5):229-37. (PMID: 19068073) ; Pediatr Infect Dis J. 2006 Jan;25(1):2-7. (PMID: 16395094) ; Anim Health Res Rev. 2001 Dec;2(2):129-40. (PMID: 11831435) ; J Med Microbiol. 2006 Sep;55(Pt 9):1159-1163. (PMID: 16914644) ; J Med Microbiol. 2012 Aug;61(Pt 8):1114-1120. (PMID: 22493278) ; J Clin Microbiol. 2017 Sep;55(9):2719-2735. (PMID: 28659315) ; J Clin Microbiol. 1995 May;33(5):1375-7. (PMID: 7615759) ; J Mol Evol. 1980 Dec;16(2):111-20. (PMID: 7463489) ; Nucleic Acids Res. 2017 Jan 4;45(D1):D566-D573. (PMID: 27789705) ; Infect Immun. 2011 May;79(5):1833-41. (PMID: 21343354) ; Am J Physiol Gastrointest Liver Physiol. 2004 Aug;287(2):G370-8. (PMID: 15075254) ; PLoS Negl Trop Dis. 2015 May 15;9(5):e0003791. (PMID: 25978422) ; Appl Environ Microbiol. 2001 Jan;67(1):484-9. (PMID: 11133487) ; Microbiology (Reading). 2010 Aug;156(Pt 8):2527-2536. (PMID: 20466763) ; Infect Immun. 2005 Mar;73(3):1441-51. (PMID: 15731042) ; mSystems. 2019 Jan 15;4(1):. (PMID: 30944874) ; J Clin Microbiol. 2010 Aug;48(8):2814-20. (PMID: 20519483) ; Microb Pathog. 2003 May;34(5):205-15. (PMID: 12732469) ; Res Microbiol. 2000 Sep;151(7):535-46. (PMID: 11037131) ; Bioinformatics. 2013 Nov 1;29(21):2669-77. (PMID: 23990416) ; J Bacteriol. 1999 May;181(9):2823-33. (PMID: 10217774) ; J Clin Invest. 2007 Feb;117(2):428-37. (PMID: 17256057) ; Int J Med Microbiol. 2016 Jun;306(4):231-6. (PMID: 27236730) ; Microbiol Mol Biol Rev. 2009 Dec;73(4):750-74. (PMID: 19946140) ; Lancet. 1978 May 27;1(8074):1119-22. (PMID: 77415) ; J Clin Microbiol. 2010 Nov;48(11):4107-14. (PMID: 20844211) ; J Clin Microbiol. 2013 Nov;51(11):3567-78. (PMID: 23966487) ; Front Cell Infect Microbiol. 2015 Mar 12;5:23. (PMID: 25815276) ; Proc Natl Acad Sci U S A. 2013 Jul 30;110(31):12810-5. (PMID: 23858472) ; Infect Immun. 2005 Jul;73(7):4385-90. (PMID: 15972534) ; Vet Microbiol. 2019 Apr;231:197-206. (PMID: 30955810) ; Infect Genet Evol. 2003 Jul;3(2):111-7. (PMID: 12809805) ; J Bacteriol. 2004 Nov;186(21):7290-301. (PMID: 15489441) ; Vet Microbiol. 2016 Nov 30;196:72-77. (PMID: 27939159) ; Proc Natl Acad Sci U S A. 1990 Oct;87(20):7839-43. (PMID: 2172966) ; BMC Microbiol. 2009 Jun 03;9:117. (PMID: 19490652) ; Cell Microbiol. 2010 Jan;12(1):31-41. (PMID: 19712078) ; PLoS One. 2015 Dec 03;10(12):e0142927. (PMID: 26632843) ; Infect Immun. 2008 Jan;76(1):361-8. (PMID: 17984209) ; Infect Immun. 1986 Aug;53(2):339-46. (PMID: 3525410) ; J Vis Exp. 2011 Jan 30;(47):. (PMID: 21307833) ; Mol Biol Evol. 2011 Oct;28(10):2731-9. (PMID: 21546353) ; Mol Microbiol. 2004 Nov;54(3):665-75. (PMID: 15491358) ; Lancet. 2013 Jul 20;382(9888):209-22. (PMID: 23680352) ; Emerg Infect Dis. 2006 Apr;12(4):597-603. (PMID: 16704807) ; Lancet. 2016 Sep 24;388(10051):1291-301. (PMID: 27673470) ; Mol Microbiol. 2005 Apr;56(2):447-64. (PMID: 15813736) ; J Microbiol Immunol Infect. 2011 Feb;44(1):27-32. (PMID: 21531349) ; Vet Microbiol. 2005 Mar 20;106(1-2):87-95. (PMID: 15737477) ; Antimicrob Agents Chemother. 2014 Jul;58(7):3895-903. (PMID: 24777092) ; Bacteriol Rev. 1977 Sep;41(3):667-710. (PMID: 334154) ; PeerJ. 2014 Apr 01;2:e332. (PMID: 24749011) ; Proc Natl Acad Sci U S A. 2006 Feb 7;103(6):1876-81. (PMID: 16446436) ; J Clin Microbiol. 1995 Mar;33(3):701-5. (PMID: 7751380) ; Nat Microbiol. 2016 Jan 18;1:15010. (PMID: 27571974) ; Cell Microbiol. 2000 Aug;2(4):305-15. (PMID: 11207587) ; J Clin Microbiol. 2006 Oct;44(10):3703-11. (PMID: 17021100) ; Bioinformatics. 2006 Nov 1;22(21):2688-90. (PMID: 16928733) ; J Clin Invest. 2001 Mar;107(5):621-9. (PMID: 11238563) ; J Clin Microbiol. 2010 Apr;48(4):1452-5. (PMID: 20181917) ; Mol Microbiol. 2006 Jun;60(5):1136-51. (PMID: 16689791) ; J Clin Microbiol. 2008 Jul;46(7):2280-90. (PMID: 18463209) ; Nucleic Acids Res. 1994 Nov 11;22(22):4673-80. (PMID: 7984417) ; PLoS Med. 2015 Dec 03;12(12):e1001921. (PMID: 26633831) ; Lab Invest. 2005 Oct;85(10):1308-24. (PMID: 16127426) ; Res Microbiol. 2000 Dec;151(10):865-71. (PMID: 11191812) Grant Information: K08 AI108794 United States AI NIAID NIH HHS; R01 AI108749 United States AI NIAID NIH HHS; T32 OD011130 United States OD NIH HHS; U19 AI110820 United States AI NIAID NIH HHS Contributed Indexing: Keywords: EPEC; animal model; enteropathogenic E. coli; phylogenetic analysis Substance Nomenclature: 0 (Escherichia coli Proteins) Entry Date(s): Date Created: 20201201 Date Completed: 20210722 Latest Revision: 20210817 Update Code: 20240513 PubMed Central ID: PMC8097272

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

Comparative Genomics of Atypical Enteropathogenic Escherichia coli from Kittens and Children Identifies Bacterial Factors Associated with Virulence in Kittens.