Einzeltreffer — DigiBib

Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), a known long noncoding RNA, was reported to play a crucial role in follicular growth and ovarian disease. However, the physiological function of MALAT1 in mouse granulosa cells (mGCs) remains largely unclear. The aims of this study were to determine the biological function and molecular mechanism of MALAT1 in mGCs. We knocked down MALAT1 in mGCs by using siRNA against MALAT1. We found that knockdown of MALAT1 promoted apoptosis and caspase-3/9 activities in mGCs. Enzyme-linked immunosorbent assay demonstrated that knockdown of MALAT1 significantly decreased the production of estradiol (E2) and progesterone (P4) in mGCs. Mechanistically, MALAT1 serves as a competing endogenous RNA (ceRNA) to sponge microRNA-205 (miR-205), thereby facilitating its downstream target of cyclic AMP response element- (CRE-) binding protein 1 (CREB1). Furthermore, CREB1 overexpression or miR-205 downregulation partially recovered the effect of MALAT1 depletion in mGCs. In summary, these findings suggested that MALAT1 regulated apoptosis and estradiol synthesis of mGCs through the miR-205/CREB1 axis.

Competing Interests: The authors declare that they have no competing interests.

Titel:	lncRNA MALAT1 Regulates Mouse Granulosa Cell Apoptosis and 17 β -Estradiol Synthesis via Regulating miR-205/CREB1 Axis.
Autor/in / Beteiligte Person:	Sun, L ; Zhang, P ; Lu, W
Link:	Full Text Finder (Volltext)
Zeitschrift:	BioMed research international, Jg. 2021 (2021-02-17), S. 6671814
Veröffentlichung:	New York, NY : Hindawi Pub. Co., 2021
Medientyp:	academicJournal
ISSN:	2314-6141 (electronic)
DOI:	10.1155/2021/6671814
Schlagwort:	Animals Cyclic AMP Response Element-Binding Protein genetics Estradiol genetics Female Mice MicroRNAs genetics RNA, Long Noncoding genetics Apoptosis Cyclic AMP Response Element-Binding Protein metabolism Estradiol biosynthesis Granulosa Cells metabolism MicroRNAs metabolism RNA, Long Noncoding metabolism Signal Transduction
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Biomed Res Int] 2021 Feb 17; Vol. 2021, pp. 6671814. <i>Date of Electronic Publication: </i>2021 Feb 17 (<i>Print Publication: </i>2021). MeSH Terms: Apoptosis* ; Signal Transduction* ; Cyclic AMP Response Element-Binding Protein / metabolism ; Estradiol / biosynthesis ; Granulosa Cells / metabolism ; MicroRNAs / metabolism ; RNA, Long Noncoding / *metabolism ; Animals ; Cyclic AMP Response Element-Binding Protein / genetics ; Estradiol / genetics ; Female ; Mice ; MicroRNAs / genetics ; RNA, Long Noncoding / genetics References: Nucleic Acids Res. 2020 May 7;48(8):4480-4491. (PMID: 32112110) ; Front Biosci (Landmark Ed). 2019 Mar 1;24:750-764. (PMID: 30844710) ; J Cell Biochem. 2020 Apr 10;:. (PMID: 32277510) ; Biol Reprod. 1981 Feb;24(1):105-13. (PMID: 7470536) ; Commun Biol. 2020 Mar 18;3(1):131. (PMID: 32188888) ; Curr Top Dev Biol. 2020;138:73-112. (PMID: 32220299) ; Anticancer Drugs. 2018 Sep;29(8):767-773. (PMID: 29916899) ; J Cell Physiol. 2019 Jul;234(7):10080-10100. (PMID: 30537129) ; Sci Rep. 2018 Feb 2;8(1):2202. (PMID: 29396444) ; Eur Rev Med Pharmacol Sci. 2020 Mar;24(5):2601-2615. (PMID: 32196610) ; Differentiation. 2020 Mar - Apr;112:1-6. (PMID: 31830612) ; BMC Mol Cell Biol. 2020 Mar 30;21(1):23. (PMID: 32228440) ; Mol Cell. 2010 Sep 24;39(6):925-38. (PMID: 20797886) ; J Cell Mol Med. 2020 Jan;24(1):451-464. (PMID: 31733099) ; Mol Reprod Dev. 2019 Aug;86(8):1023-1032. (PMID: 31179605) ; Leukemia. 2018 Oct;32(10):2250-2262. (PMID: 29632340) ; J Cell Mol Med. 2019 Jan;23(1):439-452. (PMID: 30324652) ; J Reprod Dev. 2004 Oct;50(5):493-514. (PMID: 15514456) ; J Anim Sci. 2004;82 E-Suppl:E40-52. (PMID: 15471814) ; Mol Ther Nucleic Acids. 2020 Jun 5;20:205-216. (PMID: 32179451) ; Kaohsiung J Med Sci. 2018 Sep;34(9):503-510. (PMID: 30173780) ; Reprod Biol. 2017 Dec;17(4):295-304. (PMID: 29089199) ; Nature. 2014 Jan 16;505(7483):344-52. (PMID: 24429633) ; Life Sci. 2020 Nov 1;260:118299. (PMID: 32827542) ; Cancers (Basel). 2019 Feb 13;11(2):. (PMID: 30781877) ; PLoS One. 2013;8(3):e59667. (PMID: 23527246) ; Endocrinology. 1991 Nov;129(5):2799-801. (PMID: 1718732) ; Adv Exp Med Biol. 1995;377:103-19. (PMID: 7484418) ; Mol Cell Endocrinol. 2020 Jan 1;499:110589. (PMID: 31557499) ; J Cell Biochem. 2018 Dec 16;:. (PMID: 30556190) ; Mol Cell Endocrinol. 2020 Mar 1;503:110697. (PMID: 31891769) ; FASEB J. 2020 May;34(5):6070-6085. (PMID: 32246794) ; Mol Hum Reprod. 2019 Jan 1;25(1):17-29. (PMID: 30371869) ; Biomed Pharmacother. 2018 Sep;105:1141-1146. (PMID: 30021350) ; Nat Med. 2015 Nov;21(11):1253-61. (PMID: 26540387) ; Hum Reprod. 2014 Sep;29(9):1995-2005. (PMID: 24963167) ; J Reprod Dev. 2012;58(1):44-50. (PMID: 22450284) Substance Nomenclature: 0 (Creb1 protein, mouse) ; 0 (Cyclic AMP Response Element-Binding Protein) ; 0 (MIRN205 microRNA, mouse) ; 0 (Malat1 long non-coding RNA, mouse) ; 0 (MicroRNAs) ; 0 (RNA, Long Noncoding) ; 4TI98Z838E (Estradiol) Entry Date(s): Date Created: 20210308 Date Completed: 20210525 Latest Revision: 20210525 Update Code: 20240513 PubMed Central ID: PMC7904346

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

lncRNA MALAT1 Regulates Mouse Granulosa Cell Apoptosis and 17 β -Estradiol Synthesis via Regulating miR-205/CREB1 Axis.