Expression of Wnt3, β - catenin and MMP - 7 in gastric cancer and precancerous lesions and their correlations with Helicobacter pylori infection.
In: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences, Jg. 46 (2021-06-28), Heft 6, S. 575-582
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Objectives: To compare the expression of the Wnt signaling-associated proteins (Wnt3, β-catenin, MMP-7) in gastric cancer and precancerous lesions with positive and negative Helicobacter pylori ( H.pylori , Hp ) infection, and to further explore the mechanisms underlying the Wnt signaling pathway involving in the formation of gastric cancer and its relationship with Hp infection.
Methods: The complete paraffin samples with pathologically confirmed diagnosis, who came from the First Hospital of Changsha from January 2018 to April 2020, were collected. All samples were randomly divided into a gastric cancer group ( n =57), a precancerous lesion group ( n =84), and a chronic superficial gastritis group ( n =25). Improved Giemsa staining was used to detect Hp infection, and according the results of Hp infection the above groups were divided into a Hp positive subgroup and a negative subgroup. The expressions of Wnt3, β-catenin and MMP-7 were examined with immunohistochemistry.
Results: The Wnt3, β-catenin, and MMP-7 were highly expressed in the gastric cancer group and the gastric precancerous lesion group. The Wnt3 and MMP-7 were highly expressed in cytoplasm, and β-catenin showed a tendency of cell membrane transferring to cytoplasm and nucleus, which was characterized by "nuclear translocation". The positive rates of the Wnt3, β-catenin, and MMP-7 expressions in the gastric cancer group were higher than those in the precancerous lesion group and the chronic superficial gastritis group (all P <0.05), which showed a gradually increasing trend with the deterioration of differentiation degree. In addition, the expressions of Wnt3, β-catenin, and MMP-7 in the Hp positive subgroup in the gastric cancer group and the precancerous lesion group were higher than those in the Hp negative subgroup (all P <0.05).
Conclusions: Aberrant activation of Wnt signaling pathway is involved in the occurrence and development of precancerous lesions and gastric cancer, and which is related with Hp infection. Meanwhile, the Wnt3, β-catenin and MMP-7 may be used as molecular markers for early diagnosis of gastric cancer and indicators to judge the degree of differentiation and malignancy of gastric cancer.
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Expression of Wnt3, β - catenin and MMP - 7 in gastric cancer and precancerous lesions and their correlations with Helicobacter pylori infection.
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Autor/in / Beteiligte Person: | Zhou, Z ; Ye, G ; Peng, J ; He, B ; Xu, S ; Fan, W ; Wang, W |
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Zeitschrift: | Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences, Jg. 46 (2021-06-28), Heft 6, S. 575-582 |
Veröffentlichung: | Changsha Shi : "Zhong nan da xue xue bao (yi xue ban)" bian ji bu, 2004-, 2021 |
Medientyp: | academicJournal |
ISSN: | 1672-7347 (print) |
DOI: | 10.11817/j.issn.1672-7347.2021.200733 |
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