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During quantitative real-time polymerase chain reaction (RT-qPCR) data analysis, the selection of optimal housekeeping gene is necessary to ensure the accuracy of results. It is noteworthy that housekeeping genes commonly used in adult studies may not be applicable for fetus. However, the stability analysis of housekeeping gene in fetal kidney has not been reported. This study intends to screen the applicable compound housekeeping genes in rat fetal kidney. In this study, eight housekeeping genes used in kidney studies based on literature reports (GAPDH, ACTB, 18S, HPRT, YWHAZ, HMBS, PPIA, and TBP) were selected as the research object. Their expression levels in the rat fetal kidney in physiological condition and the intrauterine growth retardation (IUGR) model induced by prenatal dexamethasone exposure (PDE) (0.2 mg/kg·day from gestation Days 9 to 20) was measured. Furthermore, these eight housekeeping genes were used to conduct relative quantitative analysis of nephrin expression in the fetal kidney in PDE-induced IUGR model, to compare the influence of choosing different housekeeping gene on data analysis of nephrin expression and to verify the reliability of selected compound housekeeping genes. In this study, stable housekeeping genes of fetal kidney tissues in PDE-induced IUGR model were identified: ACTB, GAPDH, TBP, and HMBS for males; ACTB, YWHAZ, and GAPDH for females. Besides, our results suggest that ACTB + GAPDH were the best compound housekeeping genes for normalization analysis in male fetal kidney studies, and ACTB + YWHAZ in females. This study will provide an experimental evidence basis for the selection of housekeeping genes in the RT-qPCR experiment in renal development toxicology-related models.

Titel:	The selection and identification of compound housekeeping genes for quantitative real-time polymerase chain reaction analysis in rat fetal kidney.
Autor/in / Beteiligte Person:	Zhao, X ; Chen, H ; Zhu, Y ; Liu, Y ; Gao, L ; Wang, H ; Ao, Y
Link:	Full Text Finder (Volltext)
Zeitschrift:	Journal of applied toxicology : JAT, Jg. 42 (2022-03-01), Heft 3, S. 360-370
Veröffentlichung:	<Apr. 1992- >: Chichester : John Wiley And Sons ; <i>Original Publication</i>: [Philadelphia, Pa. : Heyden & Son, c1981-, 2022
Medientyp:	academicJournal
ISSN:	1099-1263 (electronic)
DOI:	10.1002/jat.4221
Schlagwort:	Animals Fetus Rats Data Accuracy Gene Expression Genes, Essential Genetics instrumentation Kidney metabolism Real-Time Polymerase Chain Reaction instrumentation
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [J Appl Toxicol] 2022 Mar; Vol. 42 (3), pp. 360-370. <i>Date of Electronic Publication: </i>2021 Aug 10. MeSH Terms: Data Accuracy* ; Gene Expression* ; Genes, Essential* ; Genetics / instrumentation ; Kidney / metabolism ; Real-Time Polymerase Chain Reaction / *instrumentation ; Animals ; Fetus ; Rats References: Andersen, C. L., Jensen, J. L., & Ørntoft, T. F. (2004). Normalization of real-time quantitative reverse transcription-pcr data: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Research, 64(15), 5245-5250. https://doi.org/10.1158/0008-5472.Can-04-0496. ; Arya, S. K., Jain, G., Upadhyay, S. K., Sarita, S., Dixit, H. S., & Verma, P. C. (2017). Reference genes validation in Phenacoccus solenopsis under various biotic and abiotic stress conditions. Scientific Reports, 7(1), 13520. https://doi.org/10.1038/s41598-017-13925-9. ; Azuara, V., Perry, P., Sauer, S., Spivakov, M., Jørgensen, H. F., John, R. M., Gouti, M., Casanova, M., Warnes, G., Merkenschlager, M., & Fisher, A. G. (2006). Chromatin signatures of pluripotent cell lines. Nature Cell Biology, 8(5), 532-538. https://doi.org/10.1038/ncb1403. ; Bustin, S. A. (2000). Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. Journal of Molecular Endocrinology, 25(2), 169-193. https://doi.org/10.1677/jme.0.0250169. ; Bustin, S. A., Benes, V., Garson, J. A., Hellemans, J., Huggett, J., Kubista, M., Mueller, R., Nolan, T., Pfaffl, M. W., Shipley, G. L., Vandesompele, J., & Wittwer, C. T. (2009). The MIQE guidelines: Minimum information for publication of quantitative real-time pcr experiments. Clinical Chemistry, 55(4), 611-622. https://doi.org/10.1373/clinchem.2008.112797. ; Cabiati, M., Raucci, S., Caselli, C., Guzzardi, M. A., D'Amico, A., Prescimone, T., … Del Ry, S. (2012). Tissue-specific selection of stable reference genes for real-time PCR normalization in an obese rat model. Journal of Molecular Endocrinology, 48(3), 251-260. https://doi.org/10.1530/jme-12-0024. ; Chen, H., Zhu, Y., Zhao, X., He, H., Luo, J., Ao, Y., & Wang, H. (2020). Prenatal ethanol exposure increased the susceptibility of adult offspring rats to glomerulosclerosis. Toxicology Letters, 321, 44-53. https://doi.org/10.1016/j.toxlet.2019.11.026. ; Cheung, T. T., Weston, M. K., & Wilson, M. J. (2017). Selection and evaluation of reference genes for analysis of mouse (Mus musculus) sex-dimorphic brain development. PeerJ, 5, e2909. https://doi.org/10.7717/peerj.2909. ; Cui, X., Zhou, J., Qiu, J., Johnson, M. R., & Mrug, M. (2009). Validation of endogenous internal real-time PCR controls in renal tissues. American Journal of Nephrology, 30(5), 413-417. https://doi.org/10.1159/000235993. ; Dlugos, C. P., Picciotto, C., Lepa, C., Krakow, M., Stöber, A., Eddy, M. L., Weide, T., Jeibmann, A., P. Krahn, M., van Marck, V., Klingauf, J., Ricker, A., Wedlich-Söldner, R., Pavenstädt, H., Klämbt, C., & George, B. (2019). Nephrin signaling results in integrin β1 activation. Journal of the American Society of Nephrology, 30(6), 1006-1019. https://doi.org/10.1681/asn.2018040362. ; Dorey, E. S., Pantaleon, M., Weir, K. A., & Moritz, K. M. (2014). Adverse prenatal environment and kidney development: Implications for programing of adult disease. Reproduction, 147(6), R189-R198. https://doi.org/10.1530/rep-13-0478. ; Dos Santos, C. P., da Cruz Saraiva, K. D., Batista, M. C., Germano, T. A., & Costa, J. H. (2020). Identification and evaluation of reference genes for reliable normalization of real-time quantitative PCR data in acerola fruit, leaf, and flower. Molecular Biology Reports, 47(2), 953-965. https://doi.org/10.1007/s11033-019-05187-7. ; DuBois, B., Pearson, J., Hastings, B., Mahmood, T., Chan, T., Alnakhli, A., & Cherala, G. (2013). Maternal low-protein diet alters the expression of real-time quantitative polymerase chain reaction reference genes in an age-, sex-, and organ-dependent manner in rat offspring. Nutrition Research, 33(3), 235-241. https://doi.org/10.1016/j.nutres.2013.01.003. ; Fukusumi, Y., Zhang, Y., Yamagishi, R., Oda, K., Watanabe, T., Matsui, K., & Kawachi, H. (2018). Nephrin-binding ephrin-B1 at the slit diaphragm controls podocyte function through the JNK pathway. Journal of the American Society of Nephrology, 29(5), 1462-1474. https://doi.org/10.1681/asn.2017090993. ; Gholami, K., Loh, S. Y., Salleh, N., Lam, S. K., & Hoe, S. Z. (2017). Selection of suitable endogenous reference genes for qPCR in kidney and hypothalamus of rats under testosterone influence. PLoS ONE, 12(6), e0176368. https://doi.org/10.1371/journal.pone.0176368. ; Gross, M. L., Amann, K., & Ritz, E. (2005). Nephron number and renal risk in hypertension and diabetes. Journal of the American Society of Nephrology, 16(Suppl 1), S27-S29. https://doi.org/10.1681/asn.2004110967. ; Herath, S., Dai, H., Erlich, J., Au, A. Y., Taylor, K., Succar, L., & Endre, Z. H. (2020). Selection and validation of reference genes for normalisation of gene expression in ischaemic and toxicological studies in kidney disease. PLoS ONE, 15(5), e0233109. https://doi.org/10.1371/journal.pone.0233109. ; Hoy, W. E., Hughson, M. D., Bertram, J. F., Douglas-Denton, R., & Amann, K. (2005). Nephron number, hypertension, renal disease, and renal failure. Journal of the American Society of Nephrology, 16(9), 2557-2564. https://doi.org/10.1681/asn.2005020172. ; Huggett, J., Dheda, K., Bustin, S., & Zumla, A. (2005). Real-time rt-PCR normalisation; strategies and considerations. Genes and Immunity, 6(4), 279-284. https://doi.org/10.1038/sj.gene.6364190. ; Humphreys, B. D., Czerniak, S., DiRocco, D. P., Hasnain, W., Cheema, R., & Bonventre, J. V. (2011). Repair of injured proximal tubule does not involve specialized progenitors. Proceedings of the National Academy of Sciences of the United States of America, 108(22), 9226-9231. https://doi.org/10.1073/pnas.1100629108. ; Lee, C., Yiau, K. X. S., Lee, L. J., Chong, P. P., Chang, K. M., & Abdullah, M. (2019). Selection of reference genes for quantitative studies in acute myeloid leukaemia. Malaysian Journal of Pathology, 41(3), 313-326. ; Li, B., Zhu, Y., Chen, H., Gao, H., He, H., Zuo, N., Pei, L., Xie, W., Chen, L., Ao, Y., & Wang, H. (2019). Decreased H3K9ac level of AT2R mediates the developmental origin of glomerulosclerosis induced by prenatal dexamethasone exposure in male offspring rats. Toxicology, 411, 32-42. https://doi.org/10.1016/j.tox.2018.10.013. ; Little, M. H., & McMahon, A. P. (2012). Mammalian kidney development: Principles, progress, and projections. Cold Spring Harbor Perspectives in Biology, 4(5), a008300. https://doi.org/10.1101/cshperspect.a008300. ; Liu, K., Wang, G., Li, L., Chen, G., Gong, X., Zhang, Q., & Wang, H. (2020). GR-C/EBPα-IGF1 axis mediated azithromycin-induced liver developmental toxicity in fetal mice. Biochemical Pharmacology, 180, 114130. https://doi.org/10.1016/j.bcp.2020.114130. ; Marcial-Quino, J., Fierro, F., De la Mora-De la Mora, I., Enríquez-Flores, S., Gómez-Manzo, S., Vanoye-Carlo, A., … Reyes-Vivas, H. (2016). Validation of housekeeping genes as an internal control for gene expression studies in giardia lamblia using quantitative real-time PCR. Gene, 581(1), 21-30. https://doi.org/10.1016/j.gene.2016.01.018. ; Miracle, X., Di Renzo, G. C., Stark, A., Fanaroff, A., Carbonell-Estrany, X., & Saling, E. (2008). Guideline for the use of antenatal corticosteroids for fetal maturation. Journal of Perinatal Medicine, 36(3), 191-196. https://doi.org/10.1515/jpm.2008.032. ; Nascimento, C. S., Barbosa, L. T., Brito, C., Fernandes, R. P., Mann, R. S., Pinto, A. P., … Duarte, M. S. (2015). Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus). PLoS ONE, 10(5), e0127935. https://doi.org/10.1371/journal.pone.0127935. ; Oxburgh, L. (2018). Kidney nephron determination. Annual Review of Cell and Developmental Biology, 34, 427-450. https://doi.org/10.1146/annurev-cellbio-100616-060647. ; Pfaffl, M. W., Tichopad, A., Prgomet, C., & Neuvians, T. P. (2004). Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: Bestkeeper-excel-based tool using pair-wise correlations. Biotechnology Letters, 26(6), 509-515. https://doi.org/10.1023/b:bile.0000019559.84305.47. ; Reagan-Shaw, S., Nihal, M., & Ahmad, N. (2008). Dose translation from animal to human studies revisited. FASEB Journal, 22(3), 659-661. https://doi.org/10.1096/fj.07-9574LSF. ; Shankland, S. J. (2006). The podocyte's response to injury: Role in proteinuria and glomerulosclerosis. Kidney International, 69(12), 2131-2147. https://doi.org/10.1038/sj.ki.5000410. ; Sharan, R. N., Vaiphei, S. T., Nongrum, S., Keppen, J., & Ksoo, M. (2015). Consensus reference gene(s) for gene expression studies in human cancers: End of the tunnel visible? Cellular Oncology (Dordrecht), 38(6), 419-431. https://doi.org/10.1007/s13402-015-0244-6. ; Skröder, H., Hawkesworth, S., Moore, S. E., Wagatsuma, Y., Kippler, M., & Vahter, M. (2016). Prenatal lead exposure and childhood blood pressure and kidney function. Environmental Research, 151, 628-634. https://doi.org/10.1016/j.envres.2016.08.028. ; Solano, M. E., Thiele, K., Kowal, M. K., & Arck, P. C. (2016). Identification of suitable reference genes in the mouse placenta. Placenta, 39, 7-15. https://doi.org/10.1016/j.placenta.2015.12.017. ; Svingen, T., Letting, H., Hadrup, N., Hass, U., & Vinggaard, A. M. (2015). Selection of reference genes for quantitative RT-qPCR (RT-qPCR) analysis of rat tissues under physiological and toxicological conditions. PeerJ, 3, e855. https://doi.org/10.7717/peerj.855. ; Vandesompele, J., De Preter, K., Pattyn, F., Poppe, B., Van Roy, N., De Paepe, A., & Speleman, F. (2002). Accurate normalization of real-time quantitative rt-PCR data by geometric averaging of multiple internal control genes. Genome Biology, 3(7). https://doi.org/10.1186/gb-2002-3-7-research0034. ; Wang, X., Peng, F., Dong, G., Sun, Y., Dai, X., Yang, Y., Liu, X., & Bai, Z. (2018). Identification and validation of appropriate reference genes for qrt-PCR analysis in Corynebacterium glutamicum. FEMS Microbiology Letters, 365(8). https://doi.org/10.1093/femsle/fny030. ; Xiao, H., Wu, Z., Li, B., Shangguan, Y., Stoltz, J. F., Magdalou, J., Chen, L., & Wang, H. (2020). The low-expression programming of 11Β-HSD2 mediates osteoporosis susceptibility induced by prenatal caffeine exposure in male offspring rats. British Journal of Pharmacology, 177(20), 4683-4700. https://doi.org/10.1111/bph.15225. ; Zhang, C., Fu, J., Wang, Y., Bao, Z., & Zhao, H. (2015). Identification of suitable reference genes for gene expression normalization in the quantitative real-time PCR analysis of sweet osmanthus (Osmanthus fragrans lour.). PLoS ONE, 10(8), e0136355. https://doi.org/10.1371/journal.pone.0136355. ; Zhang, Q., Pei, L. G., Liu, M., Lv, F., Chen, G., & Wang, H. (2020). Reduced testicular steroidogenesis in rat offspring by prenatal nicotine exposure: Epigenetic programming and heritability via nAChR/HDAC4. Food and Chemical Toxicology, 135, 111057. https://doi.org/10.1016/j.fct.2019.111057. ; Zhu, Y., Chen, H., Zhao, X., Li, B., He, H., Cheng, H., Wang, H., & Ao, Y. (2019). Decreased H3K9ac level of KLF4 mediates podocyte developmental toxicity induced by prenatal caffeine exposure in male offspring rats. Toxicology Letters, 314, 63-74. https://doi.org/10.1016/j.toxlet.2019.07.011. Contributed Indexing: Keywords: RT-qPCR; compound housekeeping gene; fetal kidney; prenatal dexamethasone exposure; sex differences; stability analysis Entry Date(s): Date Created: 20210810 Date Completed: 20220225 Latest Revision: 20220225 Update Code: 20231215

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The selection and identification of compound housekeeping genes for quantitative real-time polymerase chain reaction analysis in rat fetal kidney.