Aroylhydrazone Diorganotin Complexes Causes DNA Damage and Apoptotic Cell Death: From Chemical Synthesis to Biochemical Effects.
In: International journal of molecular sciences, Jg. 22 (2021-12-16), Heft 24
Online
academicJournal
Zugriff:
Under microwave irradiation, eighteen new aroylhydrazone diorganotin complexes ( 1a - 9b ) were produced through the reaction of aroylhydrazine, 2-ketobutyric acid, and the corresponding diorganotin. Fourier transform infrared spectroscopy, 1 H, 13 C, and 119 Sn nuclear magnetic resonance spectroscopies, high-resolution mass spectroscopy, X-ray crystallography, and thermogravimetric analysis (TGA) were performed to characterize the complexes. The in vitro anticancer activity for complexes were assessed using a CCK-8 assay on human cancer cells of HepG2, NCI-H460, and MCF-7. Complex 4b revealed more intensive anticancer activity against MCF-7 cells than the other complexes and cisplatin. Flow cytometry analysis and transmission electron microscope observation demonstrated that complex 4b mediated cell apoptosis of MCF-7 cells and arrested cell cycle in S phase. Western blotting analysis showed that 4b induced DNA damage in MCF-7 cells and led to apoptosis by the ATM-CHK2-p53 pathway. The single cell gel electrophoreses assay results showed that 4b induced DNA damage. The DNA binding activity of 4b was studied by UV-Visible absorption spectrometry, fluorescence competitive, viscosity measurements, gel electrophoresis, and molecular docking, and the results show that 4b can be well embedded in the groove and cleave DNA.
Titel: |
Aroylhydrazone Diorganotin Complexes Causes DNA Damage and Apoptotic Cell Death: From Chemical Synthesis to Biochemical Effects.
|
---|---|
Autor/in / Beteiligte Person: | Jiang, W ; Tan, Y ; Peng, Y |
Link: | |
Zeitschrift: | International journal of molecular sciences, Jg. 22 (2021-12-16), Heft 24 |
Veröffentlichung: | Basel, Switzerland : MDPI, [2000-, 2021 |
Medientyp: | academicJournal |
ISSN: | 1422-0067 (electronic) |
DOI: | 10.3390/ijms222413525 |
Schlagwort: |
|
Sonstiges: |
|