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Background: Cardiovascular disease (CVD) is the leading cause of death globally, despite the recent advancements in clinical research. Early diagnosis of CVD and prevention of future complications are important for the management of CVD. In the present study, we determined the genotypic linkage of interleukin-6 (IL-6) promoters with the clinical, biochemical, and inflammatory markers of CVD in the Saudi population.

Materials and Methods: The study consisted of 89 patients (male and female) with CVD who were admitted at the King Abdulaziz university hospital, Jeddah, Saudi Arabia. The biochemical parameters were evaluated using an automated chemistry analyzer, and inflammatory markers were measured using specific enzyme-linked immunosorbent assay (ELISA) kits. For genotypic analysis, Sanger sequencing was performed. We observed a statistically significant association (p < 0.05) between GG (66.29%), GC (30.34%), and CC (3.37%) genotypes at the - 174G/C (rs1800795) hotspot and neopterin levels. However, the genotypes at the - 572G/C (rs1800796) hotspot did not show any association with age, gender, obesity, diabetes, hypertension, dyslipidemia, smoking, and coronary artery status. In addition, no significant association was observed with biochemical and inflammatory markers, namely fasting blood sugar, glycated hemoglobin A1c, creatinine, total cholesterol, triglycerides, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol, IL-6, and C-reactive protein. The comparison between different possible genotypic groups and CVD risk factors showed a statistically significant (p < 0.05) association between the male gender and HDL with GG, rs1800795 group vs. GC, rs1800796 group. Similarly, neopterin level was also found to be significantly (p < 0.05) associated with the genotypes GC, rs1800795, and GG, rs1800796. Additionally, the male gender (p < 0.01), age (p < 0.05), serum creatinine (p < 0.001), and neopterin (p < 0.05) were found to be significantly associated with GG, rs1800795 + GG, rs1800796, GC, rs1800795 + GC, and rs1800796 GC.

Conclusion: The direct association of neopterin level with IL-6 promoter polymorphism at - 174G/C (rs1800795) hotspot indicated the role of inflammation in CVD pathogenesis in the Saudi population.

Titel:	Association of IL-6 promoter polymorphism hotspots (- 174G/C and - 572G/C) with cardiovascular disease risk factors.
Autor/in / Beteiligte Person:	Tabrez, S ; Jabir, NR ; Zughaibi, TA ; Suhail, M
Link:	Full Text Finder (Volltext)
Zeitschrift:	Molecular biology reports, Jg. 49 (2022-03-01), Heft 3, S. 2265-2272
Veröffentlichung:	Dordrecht, Boston, Reidel., 2022
Medientyp:	academicJournal
ISSN:	1573-4978 (electronic)
DOI:	10.1007/s11033-021-07048-8
Schlagwort:	Female Genetic Predisposition to Disease Genotype Humans Male Polymorphism, Single Nucleotide genetics Promoter Regions, Genetic Risk Factors Cardiovascular Diseases genetics Interleukin-6 genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Mol Biol Rep] 2022 Mar; Vol. 49 (3), pp. 2265-2272. <i>Date of Electronic Publication: </i>2022 Jan 13. MeSH Terms: Cardiovascular Diseases* / genetics ; Interleukin-6 / *genetics ; Female ; Genetic Predisposition to Disease ; Genotype ; Humans ; Male ; Polymorphism, Single Nucleotide / genetics ; Promoter Regions, Genetic ; Risk Factors References: Virani SS, Alonso A, Benjamin EJ et al (2020) Heart disease and stroke statistics—2020 update: a report from the American Heart Association. Circulation 141(9):e139–e596. (PMID: 10.1161/CIR.0000000000000757) ; Haukilahti MAE, Kenttä TV, Tikkanen JT et al (2021) Electrocardiographic risk markers of cardiac death: gender differences in the general population. Front Physiol 11:1829. 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Association of IL-6 promoter polymorphism hotspots (- 174G/C and - 572G/C) with cardiovascular disease risk factors.