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The metabolism of polyunsaturated fatty acids (PUFAs) plays an important role in male reproduction. Linoleic and alpha-linolenic acids need to be provided in the diet and they are converted into long chain polyunsaturated fatty acids by steps of elongation and desaturation, exerted by elongases 2 (ELOVL2) and 5 (ELOVL5) and Δ5- (FADS1) and Δ6-desaturase (FADS2). This study aims to assess the gene expression and localization of enzymes involved in the synthesis of n-3 and n-6 long-chain PUFAs in control rabbits and those fed diets containing 10% extruded flaxseed. Enzyme and PUFA localization were assessed in the testes and epididymis by immunofluorescence. Testes showed high gene expression of FADS2, ELOVL2 and ELOVL5 and low expression of FADS1. Intermediate metabolites, enzymes and final products were differently found in Leydig, Sertoli and germinal cells. FADS2 was localized in interstitial cells and elongated spermatids; ELOVL5 in meiotic cells; FADS1 was evident in interstitial tissue, Sertoli cells and elongated spermatids; ELOVL2 in interstitial cells. Epididymal vesicles were positive for FADS1, ELOVL2 and ELOVL5 as well as docosahexaenoic, eicosapentaenoic, and arachidonic acids. This knowledge of fatty acids (FA) metabolism in spermatogenesis and the influence of diet on FA profile could help identify causes of male infertility, suggesting new personalized therapy.

Titel:	Expression of genes and localization of enzymes involved in polyunsaturated fatty acid synthesis in rabbit testis and epididymis.
Autor/in / Beteiligte Person:	Castellini, C ; Mattioli, S ; Moretti, E ; Cotozzolo, E ; Perini, F ; Dal Bosco, A ; Signorini, C ; Noto, D ; Belmonte, G ; Lasagna, E ; Brecchia, G ; Collodel, G
Link:	Full Text Finder (Volltext)
Zeitschrift:	Scientific reports, Jg. 12 (2022-02-16), Heft 1, S. 2637
Veröffentlichung:	London : Nature Publishing Group, copyright 2011-, 2022
Medientyp:	academicJournal
ISSN:	2045-2322 (electronic)
DOI:	10.1038/s41598-022-06700-y
Schlagwort:	Animals Diet Fatty Acids, Omega-6 biosynthesis Fatty Acids, Unsaturated metabolism Linoleic Acid metabolism Male Rabbits Spermatogenesis genetics alpha-Linolenic Acid metabolism Delta-5 Fatty Acid Desaturase genetics Delta-5 Fatty Acid Desaturase metabolism Epididymis metabolism Fatty Acid Desaturases genetics Fatty Acid Desaturases metabolism Fatty Acid Elongases genetics Fatty Acid Elongases metabolism Fatty Acids, Unsaturated biosynthesis Gene Expression Testis metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Sci Rep] 2022 Feb 16; Vol. 12 (1), pp. 2637. <i>Date of Electronic Publication: </i>2022 Feb 16. MeSH Terms: Gene Expression* ; Delta-5 Fatty Acid Desaturase / genetics ; Delta-5 Fatty Acid Desaturase / metabolism ; Epididymis / metabolism ; Fatty Acid Desaturases / genetics ; Fatty Acid Desaturases / metabolism ; Fatty Acid Elongases / genetics ; Fatty Acid Elongases / metabolism ; Fatty Acids, Unsaturated / biosynthesis ; Testis / *metabolism ; Animals ; Diet ; Fatty Acids, Omega-6 / biosynthesis ; Fatty Acids, Unsaturated / metabolism ; Linoleic Acid / metabolism ; Male ; Rabbits ; Spermatogenesis / genetics ; alpha-Linolenic Acid / metabolism References: Cell Mol Life Sci. 2019 Jul;76(14):2681-2695. (PMID: 30980107) ; Ecol Lett. 2021 Aug;24(8):1709-1731. (PMID: 34114320) ; Animal. 2017 Apr;11(4):705-712. (PMID: 27819218) ; Prog Lipid Res. 2010 Apr;49(2):186-99. (PMID: 20018209) ; J Agric Food Chem. 2016 Feb 3;64(4):792-800. (PMID: 26745387) ; Methods. 2001 Dec;25(4):402-8. (PMID: 11846609) ; Asian-Australas J Anim Sci. 2017 May;30(5):622-637. (PMID: 26954196) ; EMBO J. 2008 Sep 3;27(17):2281-92. (PMID: 19172737) ; In Vivo. 2011 Mar-Apr;25(2):197-202. (PMID: 21471535) ; Am J Clin Nutr. 2006 Jun;83(6 Suppl):1520S-1525S. (PMID: 16841862) ; Lancet. 1968 Jun 22;1(7556):1329-33. (PMID: 4172649) ; J Lipid Res. 2007 Jan;48(1):152-64. (PMID: 17050905) ; Antioxidants (Basel). 2021 Apr 27;10(5):. (PMID: 33925444) ; PLoS One. 2016 Jul 18;11(7):e0158518. (PMID: 27428262) ; Sci Rep. 2020 Aug 18;10(1):13962. (PMID: 32811870) ; Int J Mol Sci. 2020 Jul 29;21(15):. (PMID: 32751076) ; Oxid Med Cell Longev. 2020 Feb 5;2020:7038124. (PMID: 32089776) ; Biol Reprod. 2007 Aug;77(2):190-201. (PMID: 17442851) ; J Lipid Res. 2007 Jul;48(7):1592-8. (PMID: 17409318) ; J Lipid Res. 2011 Feb;52(2):245-55. (PMID: 21106902) ; Biochem J. 2000 Sep 15;350 Pt 3:765-70. (PMID: 10970790) ; Reproduction. 2007 Feb;133(2):467-77. (PMID: 17307914) ; Life Sci. 2020 Sep 15;257:118028. (PMID: 32615185) ; Reprod Fertil Dev. 2009;21(3):419-27. (PMID: 19261219) ; Reprod Fertil Dev. 2020 Jun;32(9):851-861. (PMID: 32527375) ; Lipids. 2001 Jun;36(6):601-6. (PMID: 11485164) ; Animal. 2019 Sep;13(9):1934-1942. (PMID: 30774049) ; Andrology. 2020 Jul;8(4):903-914. (PMID: 31444950) ; Anim Reprod Sci. 2016 Oct;173:63-72. (PMID: 27596261) ; Reproduction. 2019 Jan;157(1):77-85. (PMID: 30394706) ; Andrologia. 2011 Feb;43(1):38-47. (PMID: 21219381) ; Biol Reprod. 2003 Jul;69(1):117-24. (PMID: 12606372) ; Prostaglandins Leukot Essent Fatty Acids. 2006 Aug;75(2):75-9. (PMID: 16893631) ; Oxid Med Cell Longev. 2019 Dec 16;2019:3279670. (PMID: 32082475) ; Vet J. 2006 Mar;171(2):206-28. (PMID: 16490704) ; Asian J Androl. 2010 Nov;12(6):862-70. (PMID: 20729867) ; Obes Rev. 2014 Dec;15(12):996-1007. (PMID: 25346452) ; J Lipid Res. 2002 Jan;43(1):107-14. (PMID: 11792729) ; Reproduction. 2018 Dec;156(6):515-525. (PMID: 30328346) ; J Lipid Res. 2010 Feb;51(2):360-7. (PMID: 19690334) ; PLoS One. 2020 May 29;15(5):e0233322. (PMID: 32469895) ; Steroids. 2020 Nov;163:108677. (PMID: 32585208) ; Int J Biochem Cell Biol. 2002 May;34(5):525-32. (PMID: 11906823) ; Poult Sci. 1999 Mar;78(3):378-82. (PMID: 10090264) ; BMC Bioinformatics. 2012 Jun 18;13:134. (PMID: 22708584) ; Biol Reprod. 2018 Jul 1;99(1):87-100. (PMID: 29462262) ; Prostaglandins Leukot Essent Fatty Acids. 2013 Mar;88(3):227-33. (PMID: 23369366) ; Animals (Basel). 2019 Oct 15;9(10):. (PMID: 31618904) ; J Lipid Res. 2018 Jul;59(7):1175-1189. (PMID: 29724783) ; Annu Rev Nutr. 2004;24:345-76. (PMID: 15189125) ; J Biol Chem. 1999 Dec 24;274(52):37335-9. (PMID: 10601301) ; Trop Anim Health Prod. 2021 May 14;53(2):321. (PMID: 33987708) ; J Lipid Res. 2009 Sep;50(9):1870-80. (PMID: 19351970) ; Mol Reprod Dev. 2000 Mar;55(3):326-34. (PMID: 10657052) ; Heliyon. 2020 Nov 23;6(11):e05584. (PMID: 33294709) Substance Nomenclature: 0 (Delta-5 Fatty Acid Desaturase) ; 0 (Fatty Acids, Omega-6) ; 0 (Fatty Acids, Unsaturated) ; 0RBV727H71 (alpha-Linolenic Acid) ; 9KJL21T0QJ (Linoleic Acid) ; EC 1.14.19.- (Fatty Acid Desaturases) ; EC 2.3.1.- (Fatty Acid Elongases) Entry Date(s): Date Created: 20220217 Date Completed: 20220314 Latest Revision: 20220314 Update Code: 20231215 PubMed Central ID: PMC8850619

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Expression of genes and localization of enzymes involved in polyunsaturated fatty acid synthesis in rabbit testis and epididymis.