Einzeltreffer — DigiBib

The DNA damage response is a highly orchestrated process. The involvement of the DNA damage response factors in DNA damage response depends on their biochemical reactions with each other and with chromatin. Using online live-cell imaging combined with heavy ion microbeam irradiation, we studied the response of the scaffold protein X-ray repair cross-complementary protein 1 (XRCC1) at the localized DNA damage in charged particle irradiated HT1080 cells expressing XRCC1-tagged RFP. The results showed that XRCC1 was recruited to the DNA damage with ultrafast kinetics in a poly ADP-ribose polymerase-dependent manner. The consecutive reaction model well explained the response of XRCC1 at ion hits, and we found that the XRCC1 recruitment was faster and dissociation was slower in the G2 phase than those in the G1 phase. The fractionated irradiation of the same cells resulted in accelerated dissociation at the previous damage sites, and the dissociated XRCC1 immediately recycled with a higher recruitment efficiency. Our data revealed XRCC1's new rescue mechanism and its high turnover in DNA damage response, which benefits our understanding of the biochemical mechanism in DNA damage response.

Titel:	High turnover and rescue effect of XRCC1 in response to heavy charged particle radiation.
Autor/in / Beteiligte Person:	Liu, W ; Wu, R ; Guo, J ; Shen, C ; Zhao, J ; Mao, G ; Mou, H ; Zhang, L ; Du, G
Link:	Full Text Finder (Volltext)
Zeitschrift:	Biophysical journal, Jg. 121 (2022-04-19), Heft 8, S. 1493-1501
Veröffentlichung:	Cambridge, MA : Cell Press ; <i>Original Publication</i>: New York, Published by Rockefeller University Press [etc.] for the Biophysical Society., 2022
Medientyp:	academicJournal
ISSN:	1542-0086 (electronic)
DOI:	10.1016/j.bpj.2022.03.011
Schlagwort:	DNA Damage Poly(ADP-ribose) Polymerases metabolism X-Rays X-ray Repair Cross Complementing Protein 1 genetics X-ray Repair Cross Complementing Protein 1 metabolism DNA Repair DNA-Binding Proteins metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Biophys J] 2022 Apr 19; Vol. 121 (8), pp. 1493-1501. <i>Date of Electronic Publication: </i>2022 Mar 09. MeSH Terms: DNA Repair* ; DNA-Binding Proteins* / metabolism ; DNA Damage ; Poly(ADP-ribose) Polymerases / metabolism ; X-Rays ; X-ray Repair Cross Complementing Protein 1 / genetics ; X-ray Repair Cross Complementing Protein 1 / metabolism References: PLoS One. 2013;8(2):e57953. (PMID: 23469115) ; ACS Appl Mater Interfaces. 2019 Oct 16;11(41):38055-38060. (PMID: 31553570) ; Oncotarget. 2016 Jan 26;7(4):4949-60. (PMID: 26700820) ; J Biol Chem. 2009 Feb 27;284(9):5445-9. (PMID: 18940805) ; Sci Rep. 2020 Jan 29;10(1):1443. (PMID: 31996740) ; Nucleic Acids Res. 2007;35(22):7665-75. (PMID: 17982172) ; Mutat Res. 2010 Apr-Jun;704(1-3):54-60. (PMID: 19944777) ; Nucleic Acids Res. 2018 Sep 6;46(15):7747-7756. (PMID: 29955842) ; Arch Biochem Biophys. 1953 Dec;47(2):282-306. (PMID: 13114900) ; Mol Cell Biol. 1998 Jun;18(6):3563-71. (PMID: 9584196) ; Mol Cell. 2010 Oct 22;40(2):179-204. (PMID: 20965415) ; Clin Oncol (R Coll Radiol). 2020 Feb;32(2):75-83. (PMID: 31511190) ; Mol Cell. 2018 Mar 15;69(6):1046-1061.e5. (PMID: 29547717) ; DNA Repair (Amst). 2020 Sep;93:102917. (PMID: 33087283) ; Nucleic Acids Res. 2011 Aug;39(15):6489-99. (PMID: 21511815) ; Trends Biochem Sci. 2020 Apr;45(4):321-331. (PMID: 32001093) ; DNA Repair (Amst). 2018 Nov;71:177-182. (PMID: 30177435) ; Nucleic Acids Res. 2013 Mar 1;41(5):3115-29. (PMID: 23355608) ; Free Radic Biol Med. 2017 Jun;107:125-135. (PMID: 27939934) ; Nat Rev Mol Cell Biol. 2017 Oct;18(10):610-621. (PMID: 28676700) ; Sci Rep. 2019 Feb 28;9(1):3095. (PMID: 30816207) ; Curr Protoc Mol Biol. 2010 Oct;Chapter 14:Unit14.20. (PMID: 20890901) ; Nucleic Acids Res. 2017 Mar 17;45(5):2585-2599. (PMID: 27994036) ; DNA Repair (Amst). 2019 Sep;81:102664. (PMID: 31324530) ; J Biol Chem. 2000 Dec 29;275(52):40974-80. (PMID: 11016934) ; Cancers (Basel). 2019 Nov 14;11(11):. (PMID: 31739493) ; Nano Lett. 2021 Mar 24;21(6):2390-2396. (PMID: 33683892) ; Nat Methods. 2012 Jul;9(7):671-5. (PMID: 22930834) ; Cold Spring Harb Perspect Biol. 2011 Jan 01;3(1):a000745. (PMID: 20980439) ; Nature. 2019 Oct;574(7779):571-574. (PMID: 31645724) ; Rev Sci Instrum. 2016 Mar;87(3):034301. (PMID: 27036791) Substance Nomenclature: 0 (DNA-Binding Proteins) ; 0 (X-ray Repair Cross Complementing Protein 1) ; EC 2.4.2.30 (Poly(ADP-ribose) Polymerases) Entry Date(s): Date Created: 20220311 Date Completed: 20220422 Latest Revision: 20230612 Update Code: 20231215 PubMed Central ID: PMC9072578

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High turnover and rescue effect of XRCC1 in response to heavy charged particle radiation.