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In animal cells, spindle elongation during anaphase is temporally coupled with cleavage furrow formation. Spindle elongation during anaphase is regulated by NuMA/dynein/dynactin complexes that occupy the polar region of the cell membrane and are excluded from the equatorial membrane. How NuMA/dynein/dynactin are excluded from the equatorial membrane and the biological significance of this exclusion remains unknown. Here, we show that the centralspindlin (Cyk4/Mklp1) and its interacting partner RhoGEF Ect2 are required for NuMA/dynein/dynactin exclusion from the equatorial cell membrane. The Ect2-based (Ect2/Cyk4/Mklp1) and NuMA-based (NuMA/dynein/dynactin) complexes occupy mutually exclusive membrane surfaces during anaphase. The equatorial membrane enrichment of Ect2-based complexes is essential for NuMA/dynein/dynactin exclusion and proper spindle elongation. Conversely, NuMA-based complexes at the polar region of the cell membrane ensure spatially confined localization of Ect2-based complexes and thus RhoA. Overall, our work establishes that membrane compartmentalization of NuMA-based and Ect2-based complexes at the two distinct cell surfaces restricts dynein/dynactin and RhoA for coordinating spindle elongation with cleavage furrow formation.

Titel:	Membrane compartmentalization of Ect2/Cyk4/Mklp1 and NuMA/dynein regulates cleavage furrow formation.
Autor/in / Beteiligte Person:	Sana, S ; Rajeevan, A ; Kotak, S
Link:	Full Text Finder (Volltext)
Zeitschrift:	The Journal of cell biology, Jg. 221 (2022-12-05), Heft 12
Veröffentlichung:	New York : Rockefeller University Press, 2022
Medientyp:	academicJournal
ISSN:	1540-8140 (electronic)
DOI:	10.1083/jcb.202203127
Schlagwort:	Anaphase Animals Cell Cycle Proteins genetics Cell Cycle Proteins metabolism Dynactin Complex metabolism Rho Guanine Nucleotide Exchange Factors metabolism Cell Division Dyneins metabolism Microtubule-Associated Proteins genetics Microtubule-Associated Proteins metabolism Spindle Apparatus metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [J Cell Biol] 2022 Dec 05; Vol. 221 (12). <i>Date of Electronic Publication: </i>2022 Oct 05. MeSH Terms: Cell Division* ; Dyneins* / metabolism ; Microtubule-Associated Proteins* / genetics ; Microtubule-Associated Proteins* / metabolism ; Spindle Apparatus* / metabolism ; Anaphase ; Animals ; Cell Cycle Proteins / genetics ; Cell Cycle Proteins / metabolism ; Dynactin Complex / metabolism ; Rho Guanine Nucleotide Exchange Factors / metabolism References: J Cell Biol. 2005 Aug 15;170(4):571-82. (PMID: 16103226) ; EMBO J. 2013 Sep 11;32(18):2517-29. (PMID: 23921553) ; Cold Spring Harb Perspect Biol. 2015 Feb 13;7(4):a015834. (PMID: 25680833) ; Curr Biol. 2020 Aug 17;30(16):3101-3115.e11. (PMID: 32619481) ; Curr Biol. 2009 Apr 14;19(7):607-12. (PMID: 19303298) ; J Cell Biol. 2004 Jul 19;166(2):167-72. (PMID: 15263015) ; PLoS One. 2013 Jun 04;8(6):e64826. (PMID: 23750214) ; J Cell Biol. 2009 Dec 14;187(6):831-45. (PMID: 20008563) ; Proc Natl Acad Sci U S A. 2006 Apr 18;103(16):6196-201. (PMID: 16603632) ; Mol Biol Cell. 2007 Dec;18(12):4992-5003. (PMID: 17942600) ; Cell Cycle. 2010 Mar 15;9(6):1084-90. (PMID: 20410686) ; Cell. 2007 Nov 2;131(3):437-40. (PMID: 17981108) ; Annu Rev Cell Dev Biol. 2012;28:29-58. (PMID: 22804577) ; Nat Rev Cancer. 2019 Jan;19(1):32-45. (PMID: 30523339) ; Curr Biol. 2018 May 7;28(9):R570-R580. (PMID: 29738735) ; EMBO Rep. 2016 Aug;17(8):1106-30. (PMID: 27432284) ; J Cell Biol. 2008 Nov 3;183(3):457-70. (PMID: 18955555) ; Curr Opin Cell Biol. 2013 Dec;25(6):741-8. (PMID: 23958212) ; EMBO J. 2014 Aug 18;33(16):1815-30. (PMID: 24996901) ; Curr Biol. 2016 Feb 22;26(4):458-69. (PMID: 26832443) ; Mol Biol Cell. 2014 Mar;25(5):606-19. (PMID: 24371089) ; Mol Biol Cell. 2020 Oct 15;31(22):2437-2451. (PMID: 32845810) ; Mol Biol Cell. 2006 Jan;17(1):43-55. (PMID: 16236794) ; Dev Cell. 2003 Jan;4(1):29-39. (PMID: 12530961) ; J Cell Sci. 2020 Jul 31;133(14):. (PMID: 32591484) ; Dev Cell. 2015 Apr 20;33(2):204-15. (PMID: 25898168) ; J Cell Biol. 2003 Apr 28;161(2):267-80. (PMID: 12719470) ; Cell. 2013 Jul 18;154(2):391-402. (PMID: 23870127) ; Biomolecules. 2019 Feb 25;9(2):. (PMID: 30823600) ; J Cell Sci. 2016 Aug 1;129(15):3015-25. (PMID: 27335426) ; Cell Rep. 2016 Dec 6;17(10):2672-2686. (PMID: 27926870) ; Nature. 1993 Apr 1;362(6419):462-5. (PMID: 8464478) ; Proc Natl Acad Sci U S A. 2020 Jan 14;117(2):1027-1035. (PMID: 31888991) ; PLoS Biol. 2009 May 5;7(5):e1000110. (PMID: 19468300) ; Mol Biol Cell. 2012 Oct;23(20):4020-31. (PMID: 22918944) ; J Cell Biol. 2002 Jun 24;157(7):1175-86. (PMID: 12082078) ; J Cell Sci. 2006 Jul 15;119(Pt 14):3008-19. (PMID: 16803869) ; J Biol Chem. 2000 Jun 9;275(23):17233-6. (PMID: 10837491) ; Proc Natl Acad Sci U S A. 2006 Jul 11;103(28):10660-5. (PMID: 16818887) ; Mol Biol Cell. 2005 Mar;16(3):1043-55. (PMID: 15616196) ; Genes Dev. 1999 Sep 1;13(17):2301-14. (PMID: 10485851) ; Front Cell Dev Biol. 2021 Apr 01;9:653801. (PMID: 33869212) ; Development. 2017 Apr 1;144(7):1137-1145. (PMID: 28351864) ; Bioessays. 2006 Oct;28(10):983-93. (PMID: 16998826) ; Proc Natl Acad Sci U S A. 2016 Aug 23;113(34):9430-9. (PMID: 27493215) ; Annu Rev Biochem. 2019 Jun 20;88:661-689. (PMID: 30649923) ; Nat Methods. 2008 May;5(5):409-15. (PMID: 18391959) ; Proc Natl Acad Sci U S A. 2007 Mar 13;104(11):4383-8. (PMID: 17360533) ; Curr Opin Cell Biol. 2019 Oct;60:1-8. (PMID: 30954860) ; Curr Biol. 2012 Feb 7;22(3):213-9. (PMID: 22226748) ; Dev Cell. 2011 Dec 13;21(6):1104-15. (PMID: 22172673) ; J Cell Biol. 2005 Jul 4;170(1):91-101. (PMID: 15998801) ; Cell Rep. 2014 Apr 10;7(1):166-79. (PMID: 24656812) ; Cell Rep. 2021 Mar 2;34(9):108805. (PMID: 33657383) ; Mol Biol Cell. 2012 Sep;23(17):3380-90. (PMID: 22809624) ; Nat Rev Mol Cell Biol. 2021 Oct;22(10):691-708. (PMID: 34158639) ; Annu Rev Biochem. 2006;75:543-66. (PMID: 16756502) ; Angew Chem Int Ed Engl. 2014 Jul 28;53(31):8211-5. (PMID: 24954740) ; Curr Biol. 2004 Oct 5;14(19):1755-60. (PMID: 15458647) ; Mol Biol Cell. 2013 Dec;24(23):3651-62. (PMID: 24109598) Grant Information: BT/PR36084/BRB/10/1857/2020 Department of Biotechnology (DBT)-Indian Institute of Science Partnership Program; IA/I/15/2/502077 Wellcome Trust India Alliance Fellowship Substance Nomenclature: 0 (Cell Cycle Proteins) ; 0 (Dynactin Complex) ; 0 (Microtubule-Associated Proteins) ; 0 (Rho Guanine Nucleotide Exchange Factors) ; EC 3.6.4.2 (Dyneins) Entry Date(s): Date Created: 20221005 Date Completed: 20221007 Latest Revision: 20230406 Update Code: 20231215 PubMed Central ID: PMC9539458

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Membrane compartmentalization of Ect2/Cyk4/Mklp1 and NuMA/dynein regulates cleavage furrow formation.