(4-Picolylamino)-17β-Estradiol derivative and analogues induce apoptosis with death receptor trail R2/DR5 in MCF-7.
In: Chemico-biological interactions, Jg. 369 (2023-01-05), S. 110286
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Zugriff:
In order to discover more effective and less toxic drugs in the field of anti-tumor, the backbone structure of 17β-estradiol was modified, and 11 target compounds were synthesized. Compounds 5 and 10, which exhibited better anti-tumor activity and higher selectivity (more than 10-fold), were chosen for further biological investigation. Flow cytometry results indicated that 5 and 10 could arrest MCF-7 cells in the G2 phase and induce apoptosis. Immunohistochemical analysis revealed that 5 and 10 could bind to the estradiol receptor alpha in MCF-7 cells. Western blotting and real-time PCR assays were performed to detect the effects of compounds on apoptosis-related targets at the protein and gene levels. These results showed that both 5 and 10 could dosed-dependently increase the expression of Apaf-1, Bax, caspase-3,8,9 and reduce the expression levels of the anti-apoptotic factors Bcl-2 and Bcl-xL. Besides, the Human apoptosis array assay demonstrated the expression level of death receptor Trail R2/DR5 was upregulated obviously while the expression of TNF R1, IAPs and Hsp27/60/70 were downregulated. On the whole, 5 induced MCF-7 cell death through the endogenous pathway in mitochondria and the exogenous pathway with death receptor Trail R2/DR5.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Titel: |
(4-Picolylamino)-17β-Estradiol derivative and analogues induce apoptosis with death receptor trail R2/DR5 in MCF-7.
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Autor/in / Beteiligte Person: | Yin, Y ; Sun, L ; Sheng, L ; Zhang, L ; Liu, J ; Wen, X ; Mo, W ; Wang, Q ; Cheng, K |
Zeitschrift: | Chemico-biological interactions, Jg. 369 (2023-01-05), S. 110286 |
Veröffentlichung: | Limerick : Elsevier ; <i>Original Publication</i>: Amsterdam, Elsevier., 2023 |
Medientyp: | academicJournal |
ISSN: | 1872-7786 (electronic) |
DOI: | 10.1016/j.cbi.2022.110286 |
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