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The olfactory bulb (OB) plays a fundamental role in the sense of smell and has been implicated in several pathologies, including Alzheimer's disease. Despite its importance, high metabolic activity and unique laminar architecture, the OB is not frequently studied using MRS methods, likely due to the small size and challenging location. Here we present a detailed metabolic characterization of OB metabolism, in terms of both static metabolite concentrations using 1 H MRS and metabolic fluxes associated with neuro-energetics and neurotransmission by tracing the dynamic 13 C flow from intravenously administered [1,6- 13 C 2 ]-glucose, [2- 13 C]-glucose and [2- 13 C]-acetate to downstream metabolites, including [4- 13 C]-glutamate, [4- 13 C]-glutamine and [2- 13 C]-GABA. The unique laminar architecture and associated metabolism of the OB, distinctly different from that of the cerebral cortex, is characterized by elevated GABA and glutamine levels, as well as increased GABAergic and astroglial energy metabolism and neurotransmission. The results show that, despite the technical challenges, high-quality 1 H and 1 H-[ 13 C] MR spectra can be obtained from the rat OB in vivo. The derived metabolite concentrations and metabolic rates demonstrate a unique metabolic profile for the OB. The metabolic model provides a solid basis for future OB studies on functional activation or pathological conditions.

Titel:	Measurement of neuro-energetics and neurotransmission in the rat olfactory bulb using <superscript>1</superscript> H and <superscript>1</superscript> H-[ <superscript>13</superscript> C] NMR spectroscopy.
Autor/in / Beteiligte Person:	Chowdhury, GMI ; Behar, KL ; Mason, GF ; Rothman, DL ; de Graaf RA
Link:	Full Text Finder (Volltext)
Zeitschrift:	NMR in biomedicine, Jg. 37 (2024-06-01), Heft 6, S. e4957
Veröffentlichung:	Chichester : Wiley ; <i>Original Publication</i>: London : Heyden & Son, 1988-, 2024
Medientyp:	academicJournal
ISSN:	1099-1492 (electronic)
DOI:	10.1002/nbm.4957
Schlagwort:	Animals Male Energy Metabolism Rats Carbon-13 Magnetic Resonance Spectroscopy Glutamine metabolism gamma-Aminobutyric Acid metabolism Magnetic Resonance Spectroscopy methods Olfactory Bulb metabolism Olfactory Bulb diagnostic imaging Synaptic Transmission physiology Rats, Sprague-Dawley Proton Magnetic Resonance Spectroscopy
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural Language: English [NMR Biomed] 2024 Jun; Vol. 37 (6), pp. e4957. <i>Date of Electronic Publication: </i>2023 May 11. MeSH Terms: Olfactory Bulb* / metabolism ; Olfactory Bulb* / diagnostic imaging ; Synaptic Transmission* / physiology ; Rats, Sprague-Dawley* ; Proton Magnetic Resonance Spectroscopy* ; Animals ; Male ; Energy Metabolism ; Rats ; Carbon-13 Magnetic Resonance Spectroscopy ; Glutamine / metabolism ; gamma-Aminobutyric Acid / metabolism ; Magnetic Resonance Spectroscopy / methods References: Shepherd GM, Greer CA. Olfactory bulb. In: Shepherd GM, ed. The Synaptic Organization of the Brain. 3rd ed. Oxford University Press; 1990:133‐169. ; Doty RL. Olfaction in Parkinson's disease and related disorders. Neurobiol Dis. 2012;46(3):527‐552. doi:10.1016/j.nbd.2011.10.026. ; Murphy C. 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J Cereb Blood Flow Metab. 2004;24(9):972‐985. doi:10.1097/01.WCB.0000126234.16188.71. Grant Information: R21 AA018210 United States AA NIAAA NIH HHS; R01 DA021785 United States DA NIDA NIH HHS; R01 NS34813 United States GF NIH HHS; R01DK27121 United States GF NIH HHS; R01 NS034813 United States NS NINDS NIH HHS; R01 DK027121 United States DK NIDDK NIH HHS; R21 AA018210 United States GF NIH HHS; UL1 TR001863 United States TR NCATS NIH HHS; R21 AA019803 United States GF NIH HHS; R21 AA019803 United States AA NIAAA NIH HHS; R01 DA021785 United States GF NIH HHS Contributed Indexing: Keywords: 1H MRS; GABA; dynamic metabolism; olfactory bulb Substance Nomenclature: 0RH81L854J (Glutamine) ; 56-12-2 (gamma-Aminobutyric Acid) Entry Date(s): Date Created: 20230423 Date Completed: 20240508 Latest Revision: 20240510 Update Code: 20240510 PubMed Central ID: PMC10590826

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Measurement of neuro-energetics and neurotransmission in the rat olfactory bulb using <superscript>1</superscript> H and <superscript>1</superscript> H-[ <superscript>13</superscript> C] NMR spectroscopy.