Conservation of Markers and Stemness in Adipose Stem and Progenitor Cells between Cattle and Other Species.

Ishida, Y ; Mabuchi, Y ; et al.

In: International journal of molecular sciences, Jg. 24 (2023-07-25), Heft 15

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Adipose stem and progenitor cells (ASPCs) have been isolated from humans and animals for use in regenerative medicine and therapy. However, knowledge of ASPCs in other species is limited. Particularly, ASPCs in livestock are expected to enhance the fat content and meat composition. In this study, we isolated bovine ASPCs using cell surface markers. Specifically, we focused on ASPC markers in humans and experimental animals, namely CD26, CD146, and CD54. Stromal vascular fraction cells from bovine fat were separated using flow cytometry before primary culture. We evaluated the self-renewal and adipogenic potential of each fraction. We identified four cell populations: CD26-CD146+CD54+, CD26-CD146+CD54-, CD26-CD146-, and CD26+CD146-. Among them, the CD26-CD146+ fraction, particularly CD54+, demonstrated the properties of preadipocytes (PreAs), characterized by slow proliferation and a high adipogenic capacity. In conclusion, we could collect and characterize possible PreAs as CD26-CD146+CD54+ or CD26-CD146+CD54-, which are expected for in vitro bovine adipogenic assays in the future.

Titel:	Conservation of Markers and Stemness in Adipose Stem and Progenitor Cells between Cattle and Other Species.
Autor/in / Beteiligte Person:	Ishida, Y ; Mabuchi, Y ; Naraoka, Y ; Hisamatsu, D ; Akazawa, C
Link:	Full Text Finder (Volltext)
Zeitschrift:	International journal of molecular sciences, Jg. 24 (2023-07-25), Heft 15
Veröffentlichung:	Basel, Switzerland : MDPI, [2000-, 2023
Medientyp:	academicJournal
ISSN:	1422-0067 (electronic)
DOI:	10.3390/ijms241511908
Schlagwort:	Humans Cattle Animals Cell Differentiation CD146 Antigen metabolism Flow Cytometry Obesity metabolism Adipose Tissue metabolism Dipeptidyl Peptidase 4 metabolism Stem Cells metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Int J Mol Sci] 2023 Jul 25; Vol. 24 (15). <i>Date of Electronic Publication: </i>2023 Jul 25. MeSH Terms: Dipeptidyl Peptidase 4* / metabolism ; Stem Cells* / metabolism ; Humans ; Cattle ; Animals ; Cell Differentiation ; CD146 Antigen / metabolism ; Flow Cytometry ; Obesity / metabolism ; Adipose Tissue / metabolism References: Animal. 2018 Feb 22;:1-7. (PMID: 29467043) ; iScience. 2022 Dec 17;26(1):105822. (PMID: 36636339) ; J Tissue Eng Regen Med. 2017 Sep;11(9):2490-2502. (PMID: 26833948) ; J Anim Sci. 2008 Jan;86(1):73-82. (PMID: 17911240) ; Stem Cell Res Ther. 2019 Jan 24;10(1):44. (PMID: 30678726) ; Cells. 2021 Sep 21;10(9):. (PMID: 34572147) ; Nature. 2018 Jul;559(7712):103-108. (PMID: 29925944) ; Cytotherapy. 2006;8(4):315-7. (PMID: 16923606) ; Trends Cell Biol. 2020 Dec;30(12):937-950. (PMID: 33148396) ; Signal Transduct Target Ther. 2020 Aug 11;5(1):148. (PMID: 32782280) ; J Cell Physiol. 2022 Jan;237(1):589-602. (PMID: 34287857) ; Cell. 2008 Oct 17;135(2):240-9. (PMID: 18835024) ; Mol Biol Evol. 2020 Apr 1;37(4):1237-1239. (PMID: 31904846) ; Nat Methods. 2012 Jul;9(7):671-5. (PMID: 22930834) ; Mol Biol Evol. 2018 Jun 1;35(6):1547-1549. (PMID: 29722887) ; Cell Mol Biol Lett. 2017 Mar 23;22:6. (PMID: 28536637) ; Vet Immunol Immunopathol. 2001 Jan 26;78(2):197-206. (PMID: 11182157) ; Science. 2019 Apr 26;364(6438):. (PMID: 31023895) ; Curr Protoc Cell Biol. 2007 Jun;Chapter 24:Unit 24.2. (PMID: 18228510) ; Genet Mol Res. 2015 Jan 15;14(1):53-62. (PMID: 25729935) ; Acta Biomater. 2011 Feb;7(2):463-77. (PMID: 20688199) ; Vet Immunol Immunopathol. 2007 Sep 15;119(1-2):106-14. (PMID: 17675249) ; Adv Sci (Weinh). 2021 Jan 27;8(6):2004032. (PMID: 33747748) Grant Information: JPMJMI18CB Japan Science and Technology Agency; JP21H03328 Otsuka (Japan) Contributed Indexing: Keywords: adipogenic stem cells; adipose stem and progenitor cells; bovine; conservation; preadipocytes Substance Nomenclature: EC 3.4.14.5 (Dipeptidyl Peptidase 4) ; 0 (CD146 Antigen) Entry Date(s): Date Created: 20230812 Date Completed: 20230814 Latest Revision: 20230814 Update Code: 20231215 PubMed Central ID: PMC10418360

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