Functional analysis of Collagen 17a1: A genetic modifier of junctional epidermolysis bullosa in mice.
In: PloS one, Jg. 18 (2023-10-05), Heft 10, S. e0292456
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Zugriff:
Previous work strongly implicated Collagen 17a1 (Col17a1) as a potent genetic modifier of junctional epidermolysis bullosa (JEB) caused by a hypomorphic mutation (Lamc2jeb) in mice. The importance of the noncollagenous domain (NC4) of COLXVII was suggested by use of a congenic reduction approach that restricted the modifier effect to 2-3 neighboring amino acid changes in that domain. The current study utilizes TALEN and CRISPR/Cas9 induced amino acid replacements and in-frame indels nested to NC4 to further investigate the role of this and adjoining COLXVII domains both as modifiers and primary risk effectors. We confirm the importance of COLXVI AA 1275 S/G and 1277 N/S substitutions and utilize small nested indels to show that subtle changes in this microdomain attenuate JEB. We further show that large in-frame indels removing up to 1482 bp and 169 AA of NC6 through NC1 domains are surprisingly disease free on their own but can be very potent modifiers of Lamc2jeb/jeb JEB. Together these studies exploiting gene editing to functionally dissect the Col17a1 modifier demonstrate the importance of epistatic interactions between a primary disease-causing mutation in one gene and innocuous 'healthy' alleles in other genes.
Competing Interests: TJS, RYW, BEL, KAS, DR, JKJ, MVW, JPS and DCR have no competing interest. JKJ has, or had during the course of this research, financial interests in several companies developing gene editing technology: Beam Therapeutics, Blink Therapeutics, Chroma Medicine, Editas Medicine, EpiLogic Therapeutics, Excelsior Genomics, Hera Biolabs, Horizon Discovery, Monitor Biotechnologies, Nvelop Therapeutics (f/k/a/ ETx, Inc.), Pairwise Plants, Poseida Therapeutics, SeQure Dx, Inc., Transposagen Biopharmaceuticals, and Verve Therapeutics. JKJ’s interests were reviewed and are managed by Massachusetts General Hospital and Mass General Brigham in accordance with their conflict of interest policies. JKJ is a co-inventor on various patents and patent applications that describe gene editing and epigenetic editing technologies. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
(Copyright: © 2023 Sproule et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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Functional analysis of Collagen 17a1: A genetic modifier of junctional epidermolysis bullosa in mice.
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Autor/in / Beteiligte Person: | Sproule, TJ ; Wilpan, RY ; Low, BE ; Silva, KA ; Reyon, D ; Joung, JK ; Wiles, MV ; Roopenian, DC ; Sundberg, JP |
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Zeitschrift: | PloS one, Jg. 18 (2023-10-05), Heft 10, S. e0292456 |
Veröffentlichung: | San Francisco, CA : Public Library of Science, 2023 |
Medientyp: | academicJournal |
ISSN: | 1932-6203 (electronic) |
DOI: | 10.1371/journal.pone.0292456 |
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