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Enhanced invasion and migration of non-small cell lung cancer (NSCLC) cells is the major cause of metastasis and poor prognosis in NSCLC. This study was conducted to investigate the role and mechanism of lncRNA KCNQ1OT1 in the proliferation, invasion, and migration of NSCLC cells. The expression of KCNQ1OT1 in NSCLC was analyzed in the StarBase database, and the target miRNA of KCNQ1OT1 as well as the target genes of the miRNA was predicted. Then, the mRNA expression levels of KCNQ1OT1, miR-496, and HMGB1 were detected in clinical tissue samples and cells by qRT-PCR assay. Besides, the protein levels of HMGB1 were detected by Western blot. MTT assay, transwell assay, and scratch assay were used to determine the proliferation, invasion, and migration ability of NSCLC cells, respectively. Correlation analysis was performed to assess the correlation between the expression of KCNQ1OT1, miR-496, and HMGB1 in clinical NSCLC samples. Dual-luciferase reporter gene assay was conducted to analyze the interaction between KCNQ1OT1 and miR-496 and between miR-496 and HMGB1. The database results showed that KCNQ1OT1 was highly expressed in NSCLC. Similarly, we found that the expression level of KCNQ1OT1 was significantly higher in NSCLC tissues and cells than that in the corresponding normal tissues and cells. The results of MTT assay, transwell assay, and scratch assay demonstrated that KCNQ1OT1 significantly enhanced the proliferation, invasion, and migration of NSCLC cells. Further mechanism exploration revealed that KCNQ1OT1 could sponge miR-496, and miR-496 directly targeted and regulated the expression of HMGB1. The expression of miR-496 and either KCNQ1OT1 or HMGB1 were negatively correlated in NSCLC, while the expression of KCNQ1OT1 and HMGB1 were positively correlated. Compared with normal paracancer tissues, miR-496 was much lower and HMGB1 was much higher expressed in NSCLC tissues. The results of cotransfection also further demonstrated that miR-496 inhibitor or sh-HMGB1 cotransfected with sh-KCNQ1OT1 could significantly decrease or increase the ability of sh-KCNQ1OT1 to inhibit the proliferation, invasion, and migration of H1299 cells, respectively. In conclusion, lncRNA KCNQ1OT1 promotes the invasion and migration of NSCLC cells through miR-496/HMGB1 signaling axis.

Titel:	LncRNA KCNQ1OT1/miR-496/HMGB1 Signaling Axis Promotes Invasion and Migration of Non-small Cell Lung Cancer Cells.
Autor/in / Beteiligte Person:	Wang, Y ; Jiang, XY ; Qu, MY ; Liang, J ; Yang, JS ; Sun, RL
Link:	Full Text Finder (Volltext)
Zeitschrift:	Biochemical genetics, Jg. 62 (2024-06-01), Heft 3, S. 1994-2009
Veröffentlichung:	1999- : New York : Kluwer Academic/Plenum Publishers ; <i>Original Publication</i>: New York, Plenum Press., 2024
Medientyp:	academicJournal
ISSN:	1573-4927 (electronic)
DOI:	10.1007/s10528-023-10526-2
Schlagwort:	Humans RNA, Long Noncoding genetics RNA, Long Noncoding metabolism Cell Proliferation Gene Expression Regulation, Neoplastic Cell Line, Tumor A549 Cells Carcinoma, Non-Small-Cell Lung genetics Carcinoma, Non-Small-Cell Lung pathology Carcinoma, Non-Small-Cell Lung metabolism MicroRNAs genetics MicroRNAs metabolism HMGB1 Protein genetics HMGB1 Protein metabolism Lung Neoplasms genetics Lung Neoplasms pathology Lung Neoplasms metabolism Cell Movement Neoplasm Invasiveness Potassium Channels, Voltage-Gated genetics Potassium Channels, Voltage-Gated metabolism Signal Transduction
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Biochem Genet] 2024 Jun; Vol. 62 (3), pp. 1994-2009. <i>Date of Electronic Publication: </i>2023 Oct 09. MeSH Terms: Carcinoma, Non-Small-Cell Lung* / genetics ; Carcinoma, Non-Small-Cell Lung* / pathology ; Carcinoma, Non-Small-Cell Lung* / metabolism ; MicroRNAs* / genetics ; MicroRNAs* / metabolism ; HMGB1 Protein* / genetics ; HMGB1 Protein* / metabolism ; Lung Neoplasms* / genetics ; Lung Neoplasms* / pathology ; Lung Neoplasms* / metabolism ; Cell Movement* ; Neoplasm Invasiveness* ; Potassium Channels, Voltage-Gated* / genetics ; Potassium Channels, Voltage-Gated* / metabolism ; Signal Transduction* ; Humans ; RNA, Long Noncoding / genetics ; RNA, Long Noncoding / metabolism ; Cell Proliferation ; Gene Expression Regulation, Neoplastic ; Cell Line, Tumor ; A549 Cells References: Albuquerque C, Manguinhas R, Costa JG et al (2021) A narrative review of the migration and invasion features of non-small cell lung cancer cells upon xenobiotic exposure: insights from in vitro studies. Transl Lung Cancer Res 10(6):2698–2714. 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LncRNA KCNQ1OT1/miR-496/HMGB1 Signaling Axis Promotes Invasion and Migration of Non-small Cell Lung Cancer Cells.