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Recent research has explored the potential use of serum-derived biomarkers in cancer screening, and mounting evidence has illustrated the pivotal roles of long noncoding RNAs (lncRNAs) in regulating laryngeal squamous cell carcinoma (LSCC) progression. LINC02191 is a newly identified lncRNA and no studies have investigated its role in malignant tumors. This study aims to explore the functions and mechanisms of lncRNA LINC02191 in LSCC. LINC02191 was knocked down in LSCC cells using shRNAs for loss-of-function experiments. RT-qPCR revealed that LINC02191 was upregulated in LSCC patients' serum exosomes, tissues and cells. Western blotting and RT-qPCR were implemented for detecting molecular protein and RNA levels. Colony formation, CCK-8, wound healing and Transwell assays were employed for examining LSCC cell malignant behaviors in vitro. A tumor-bearing mouse model (n = 4/group) was established for examining LINC02191 role in vivo. The results showed that LINC02191 silencing hindered LSCC cell proliferation, invasiveness, migration as well as EMT in vitro and impeded tumorigenesis in xenograft mouse model. Luciferase reporter assay was utilized for verifying the interaction between LINC02191, miR-204-5p and RAB22A. Pearson correlation analysis was employed to evaluate their expression correlation in LSCC tissue specimens (N = 30). Mechanistically, LINC02191 upregulated RAB22A by binding to miR-204-5p, and knocking down LINC02191 inhibited PI3K/Akt/mTOR signaling transduction in LSCC cells and tumor-bearing mice. Moreover, RAB22A overexpression reversed LINC02191 depletion-triggered suppression of LSCC cell aggressiveness and inactivation of PI3K/Akt/mTOR signaling. In conclusion, LINC02191 aggravates LSCC by targeting miR-204-5p/RAB22A/PI3K/Akt/mTOR signaling pathway, which indicates that LINC02191 may serve as a promising target for LSCC treatment.

Titel:	Exosomal lncRNA LINC02191 Promotes Laryngeal Squamous cell Carcinoma Progression by Targeting miR-204-5p/RAB22A Axis and Regulating PI3K/Akt/mTOR Pathway.
Autor/in / Beteiligte Person:	Kang, Z ; Zhang, C ; Huangfu, H
Link:	Full Text Finder (Volltext)
Zeitschrift:	Biochemical genetics, Jg. 62 (2024-06-01), Heft 3, S. 2117-2133
Veröffentlichung:	1999- : New York : Kluwer Academic/Plenum Publishers ; <i>Original Publication</i>: New York, Plenum Press., 2024
Medientyp:	academicJournal
ISSN:	1573-4927 (electronic)
DOI:	10.1007/s10528-023-10541-3
Schlagwort:	Humans Mice Animals Cell Line, Tumor Male Disease Progression Mice, Nude Female Gene Expression Regulation, Neoplastic Mice, Inbred BALB C Cell Proliferation Middle Aged MicroRNAs genetics MicroRNAs metabolism rab GTP-Binding Proteins genetics rab GTP-Binding Proteins metabolism RNA, Long Noncoding genetics RNA, Long Noncoding metabolism TOR Serine-Threonine Kinases metabolism TOR Serine-Threonine Kinases genetics Laryngeal Neoplasms genetics Laryngeal Neoplasms pathology Laryngeal Neoplasms metabolism Proto-Oncogene Proteins c-akt metabolism Phosphatidylinositol 3-Kinases metabolism Exosomes metabolism Exosomes genetics Signal Transduction
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Biochem Genet] 2024 Jun; Vol. 62 (3), pp. 2117-2133. <i>Date of Electronic Publication: </i>2023 Oct 20. MeSH Terms: MicroRNAs* / genetics ; MicroRNAs* / metabolism ; rab GTP-Binding Proteins* / genetics ; rab GTP-Binding Proteins* / metabolism ; RNA, Long Noncoding* / genetics ; RNA, Long Noncoding* / metabolism ; TOR Serine-Threonine Kinases* / metabolism ; TOR Serine-Threonine Kinases* / genetics ; Laryngeal Neoplasms* / genetics ; Laryngeal Neoplasms* / pathology ; Laryngeal Neoplasms* / metabolism ; Proto-Oncogene Proteins c-akt* / metabolism ; Phosphatidylinositol 3-Kinases* / metabolism ; Exosomes* / metabolism ; Exosomes* / genetics ; Signal Transduction* ; Humans ; Mice ; Animals ; Cell Line, Tumor ; Male ; Disease Progression ; Mice, Nude ; Female ; Gene Expression Regulation, Neoplastic ; Mice, Inbred BALB C ; Cell Proliferation ; Middle Aged References: Ali Syeda Z et al (2020) Regulatory mechanism of MicroRNA expression in Cancer. Int J Mol Sci, 21(5). ; Bai Y et al (2019) Comprehensive analysis of a ceRNA network reveals potential prognostic cytoplasmic lncRNAs involved in HCC progression. J Cell Physiol 234(10):18837–18848. (PMID: 10.1002/jcp.28522309164066618076) ; Bridges MC, Daulagala AC, Kourtidis A (2021) LNCcation: lncRNA localization and function. J Cell Biol 220(2):e202009045. (PMID: 10.1083/jcb.202009045334642997816648) ; Cavaliere M et al (2021) Biomarkers of laryngeal squamous cell carcinoma: a review. Ann Diagn Pathol 54:151787. (PMID: 10.1016/j.anndiagpath.2021.15178734242969) ; Feng Z et al (2022) The LINC00623/NAT10 signaling axis promotes pancreatic cancer progression by remodeling ac4C modification of mRNA. J Hematol Oncol 15(1):112. (PMID: 10.1186/s13045-022-01338-9359783329387035) ; Gong X, Tang H, Yang K (2021) PER1 suppresses glycolysis and cell proliferation in oral squamous cell carcinoma via the PER1/RACK1/PI3K signaling complex. Cell Death Dis 12(3):276. (PMID: 10.1038/s41419-021-03563-5337232217960720) ; Huang Y, Zhang C, Zhou Y (2019) LncRNA MIR100HG promotes cancer cell proliferation, migration and invasion in laryngeal squamous cell carcinoma through the downregulation of miR-204-5p. Onco Targets Ther 12:2967–2973. (PMID: 10.2147/OTT.S202528311142406489621) ; Li Y et al (2015) Circular RNA is enriched and stable in exosomes: a promising biomarker for cancer diagnosis. Cell Res 25(8):981–984. (PMID: 10.1038/cr.2015.82261386774528056) ; Liu Q, Lei C (2021) LINC01232 serves as a novel biomarker and promotes tumour progression by sponging mir-204-5p and upregulating RAB22A in clear cell renal cell carcinoma. Ann Med 53(1):2153–2164. (PMID: 10.1080/07853890.2021.2001563347836228604453) ; Lu TX, Rothenberg ME (2018) MicroRNA J Allergy Clin Immunol 141(4):1202–1207. (PMID: 10.1016/j.jaci.2017.08.03429074454) ; Luo X et al (2022) Rab22a promotes epithelial-mesenchymal transition in papillary thyroid carcinoma by activating PI3K/AKT/mTOR signaling pathway. Biomed Res Int 2022:1874550. (PMID: 10.1155/2022/1874550357574709217539) ; Miricescu D et al (2020) PI3K/AKT/mTOR signaling pathway in breast Cancer: from Molecular Landscape to clinical aspects. Int J Mol Sci, 22(1). ; Pastushenko I, Blanpain C (2019) EMT Transition States during Tumor Progression and Metastasis. Trends Cell Biol 29(3):212–226. (PMID: 10.1016/j.tcb.2018.12.00130594349) ; Saliminejad K et al (2019) An overview of microRNAs: Biology, functions, therapeutics, and analysis methods. J Cell Physiol 234(5):5451–5465. (PMID: 10.1002/jcp.2748630471116) ; Siegel RL et al (2023) Cancer statistics, 2023. CA Cancer J Clin 73(1):17–48. (PMID: 10.3322/caac.2176336633525) ; Song F, Yang Y, Liu J (2021) Long non-coding RNA MIAT promotes the proliferation and invasion of laryngeal squamous cell carcinoma cells by sponging microRNA-613. Exp Ther Med 21(3):232. (PMID: 10.3892/etm.2021.9663336038407851618) ; St Laurent G, Wahlestedt C, Kapranov P (2015) The Landscape of long noncoding RNA classification. Trends Genet 31(5):239–251. (PMID: 10.1016/j.tig.2015.03.007258699994417002) ; Su F et al (2016) RAB22A overexpression promotes the tumor growth of melanoma. Oncotarget 7(44):71744–71753. (PMID: 10.18632/oncotarget.12329276902215342118) ; Su Q et al (2022) miR-204-5p Hampers Breast Cancer Malignancy and Affects the Cell Cycle by Targeting PRR11 Comput Math Methods Med, 2022: p. 4010947. ; Sun X et al (2020) miRNA-520c-3p accelerates progression of nasopharyngeal carcinoma via targeting RAB22A. Oncol Lett 20(6):319. (PMID: 10.3892/ol.2020.12182330939237573920) ; Tu J et al (2021) Circular RNA Circ0021205 promotes Cholangiocarcinoma Progression through MiR-204-5p/RAB22A Axis. Front Cell Dev Biol 9:653207. (PMID: 10.3389/fcell.2021.653207340129648126682) ; Wa Q et al (2019) Mir-204-5p represses bone metastasis via inactivating NF-κB signaling in prostate Cancer. Mol Ther Nucleic Acids 18:567–579. (PMID: 10.1016/j.omtn.2019.09.008316787336838892) ; Wang C et al (2019) LncRNA DLEU1/microRNA-300/RAB22A axis regulates migration and invasion of breast cancer cells. Eur Rev Med Pharmacol Sci 23(23):10410–10421. (PMID: 31841195) ; Wang X et al (2020a) Regulation of laryngeal squamous cell cancer progression by the lncRNA RP11-159K7.2/miR-206/DNMT3A axis. J Cell Mol Med 24(12):6781–6795. (PMID: 10.1111/jcmm.15331323636887299727) ; Wang H et al (2020b) Long non-coding RNA OIP5-AS1 serves as an oncogene in laryngeal squamous cell carcinoma by regulating miR-204-5p/ZEB1 axis. Naunyn Schmiedebergs Arch Pharmacol 393(11):2177–2184. (PMID: 10.1007/s00210-020-01811-732009213) ; Wang J et al (2022) Rab22a promotes the proliferation, migration, and invasion of lung adenocarcinoma via up-regulating PI3K/Akt/mTOR signaling pathway. Exp Cell Res 416(2):113179. (PMID: 10.1016/j.yexcr.2022.11317935487271) ; Wu T et al (2022) HPRT1 promotes chemoresistance in oral squamous cell Carcinoma via activating MMP1/PI3K/Akt signaling pathway. Cancers (Basel), 14(4). ; Yang S et al (2020) miR–204–5p promotes apoptosis and inhibits migration of gastric cancer cells by targeting HER–2. Mol Med Rep 22(4):2645–2654. (PMID: 329454257453524) ; Yang X et al (2021) LncRNA LINC-PINT inhibits malignant behaviors of laryngeal squamous cell carcinoma cells via inhibiting ZEB1. Pathol Oncol Res 27:584466. (PMID: 10.3389/pore.2021.584466342575318262191) ; Yin Y et al (2014) Mir-204-5p inhibits proliferation and invasion and enhances chemotherapeutic sensitivity of colorectal cancer cells by downregulating RAB22A. Clin Cancer Res 20(23):6187–6199. (PMID: 10.1158/1078-0432.CCR-14-103025294901) ; Yin C et al (2022) Overexpression of laminin 5γ2 chain correlates with Tumor Cell Proliferation, Invasion, and poor prognosis in laryngeal squamous cell carcinoma. J Oncol 2022:7248064. (PMID: 10.1155/2022/7248064362846349588344) ; Yu L et al (2023) HDAC5-mediated PRAME regulates the proliferation, migration, invasion, and EMT of laryngeal squamous cell carcinoma via the PI3K/AKT/mTOR signaling pathway. Open Med (Wars) 18(1):20230665. (PMID: 10.1515/med-2023-066536910848) ; Zhang X et al (2015) Exosomes in cancer: small particle, big player. J Hematol Oncol 8:83. (PMID: 10.1186/s13045-015-0181-x261565174496882) ; Zhao R et al (2018) Exosomal long noncoding RNA HOTTIP as potential novel diagnostic and prognostic biomarker test for gastric cancer. Mol Cancer 17(1):68. (PMID: 10.1186/s12943-018-0817-x294867946389063) ; Zhu X, Zhu R (2018) Curcumin suppresses the progression of laryngeal squamous cell carcinoma through the upregulation of miR-145 and inhibition of the PI3K/Akt/mTOR pathway. Onco Targets Ther 11:3521–3531. (PMID: 10.2147/OTT.S159236299508576016259) Contributed Indexing: Keywords: LINC02191; Laryngeal squamous cell carcinoma; PI3K/Akt/mTOR pathway; RAB22A; miR-204-5p Substance Nomenclature: 0 (MicroRNAs) ; EC 3.6.5.2 (rab GTP-Binding Proteins) ; 0 (RNA, Long Noncoding) ; EC 2.7.11.1 (TOR Serine-Threonine Kinases) ; EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) ; EC 2.7.1.1 (MTOR protein, human) ; EC 2.7.1.- (Phosphatidylinositol 3-Kinases) ; 0 (RAB22A protein, human) ; 0 (MIRN204 microRNA, human) Entry Date(s): Date Created: 20231020 Date Completed: 20240619 Latest Revision: 20240619 Update Code: 20240620

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Exosomal lncRNA LINC02191 Promotes Laryngeal Squamous cell Carcinoma Progression by Targeting miR-204-5p/RAB22A Axis and Regulating PI3K/Akt/mTOR Pathway.